Determining Composition of Volatiles in Couroupita guianensis Aubl. Through Headspace-Solid Phase Micro-Extraction (HS-SPME)
DOI:
https://doi.org/10.24154/jhs.v9i2.188Keywords:
Couroupita guianensis, Volatiles, Headspace-Solid Phase Micro-Extraction (HS-SPME), Capillary Gas Chromatography and Mass Spectrometry (GC/MS)Abstract
Composition of volatile components in Couroupita guianensis Aubl. flowers was analyzed using headspace-solid phase micro-extraction (HS-SPME), followed by capillary gas chromatography and mass spectrometry (GC-MS) separation and identification. In all, 75 compounds were identified accounting for 96.32% of the total volatiles present. The major groups of compounds present were oxygenated terpenoids (35.66%), alcohols (26.92%), esters (17.36%), mono-and sesqui-terpenoids (8.64%), aldehydes and ketones (4.71%), hydrocarbons (1.68%), phenols (0.18%), acids (0.754%) and heterocyclic compounds (0.42%) constituted a small proportion of the volatile profile. The most abundant individual constituent was eugenol (18.95%) followed by nerol (13.49%), (E,E) farnesol (12.88%), (E,E)-farnesyl acetate (6.68%), trans ocimene (6.02%), nootkatone (4.64%), geraniol (2.94%), 2-isopropenyl-5- methyl-4-hexenyl acetate (2.69%), cedr-8-en-13-ol (2.58%), (E,Z)-farnesyl acetate (2.40%) and methyl (11E)-11- hexadecenoate (2.041%). Analytical comparison of composition of volatiles in the flowers, obtained by different methods of extraction, viz., solvent extraction, micro-simultaneous extraction and headspace-solid phase microextraction, revealed specific variations in relative concentrations of the constituent chemicals. Linalool was the major chemical (21.5% and 14.9%) in solvent extract and micro-simultaneous extract, respectively, but appeared in negligible quantity (0.16%) in head-space analysis.
References
Andrade, E.H.A., Zoghbi, M.G.B. and Maia, J.G.S. 2000. The volatiles from flowers of Couroupita guianensis Aubl., Lecythisus itata Miers. var. paraensis (Ducke) R. Kunth. and Eschweilera coriacea (A. PI DC.) Mori (Lecythidaceae). J. Essent. Oil Res., 12:163-166
Anonymous. 1950. Wealth of India. CSIR, New Delhi, 2:362 Belliardo, F., Bicchi, C., Cordero, C., Liberto, E., Rubiolo, P. and Sgorbini, B. 2006. Headspace-solid-phase microextraction in the analysis of volatile fraction of aromatic and medicinal plants. J. Chromatogr. Sci., 44:416-429
Bergman, J., Lindstrom, J.O. and Tilstam, U. 1985. The structure and properties of some indolic constituents in Couroupita guianensis Aubl. Tetrahedron, 41:2879-2881
Deng, C., Song, G. and Hub, Y. 2004. Application of HS- SPME and GC-MS to characterization of volatile compounds emitted from Osmanthus flowers. Annali di Chimica, 94:921–927
Flamini, G., Cioni, P.L. and Morelli, I. 2003. Use of solid- phase micro-extraction as a sampling technique in the determination of volatiles emitted by flowers, isolated flower parts and pollen. J. Chromatogr. A, 998:229-233
Heywood, V.H. and Chant, S.R. 1982. In: Popular Encyclopedia of Plants, Cambridge University Press, Cambridge, London. pp. 103
Kovats, E. 1965. Gas chromatographic characterization of organic substances in the retention index system. Adv. Chromatogr., 1:229-247
Mariana, M.G.P., Sidnei, O.B., Catharina, E.F., Ricardo, M.K., Maria, E.M., Fabio, S.M. and Patricia, D.F. 2010. Antinociceptive activity of fractions from Couroupita guianensis Aubl. leaves. J. Ethnopharma., 127:407–413
Pawliszyn, J. 1997. Solid phase micro-extraction. Theory and practice. New York: Wiley-VCH.
Rajamanickam, V., Rajasekaran, A., Darlinquine, S., Jesupillai, M. and Sabitha, R. 2009. Anthelmintic activity of the flower extract of Couroupita guianensis. The Internet J. Alternative Med., 8:107-111
Rane, J.B., Vahanwala, S.J., Golatkar, S.G., Ambaye, R.Y. and Khadse, B.G. 2001. Chemical examination of the flowers of Couroupita guianensis Aubl. Indian J. Pharm. Sci., 63:72-73
Stanz, B.J., Campos-de-la, C.J., Epiquien, R.M.A. and Canigueral, S. 2009. A first survey on the medicinal plants of the Chazuta valley (Peruvian Amazon). J. Ethnopharma., 122:333–362
Satyavati, G.V., Raina, M.K. and Sharma, M. 1976. In: Medicinal Plants of India, ICMR, Vol.-1, Cambridge Printing Works New Delhi, India, p. 286
Sen, A.K., Mahato, S.B. and Dutta, N.L. 1974. Couroupitine A, a new alkaloid from Couroupita guianensis. Tetrahedron Lett., 7:609-610
Wong, K.C. and Tie, D.Y. 1995. Volatile constituents of Couroupita guianensis Aubl. flowers. J. Essent. Oil Res., 7:225-227
Downloads
Published
Issue
Section
License
Copyright (c) 2014 Arpita Mandal Khan, K S Shivashankara, T K Roy (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.