Morpho-physiological characterization of second generation colchiploids in sweet orange (Citrus sinensis (L.) Osbeck) cv. Mosambi

Authors

  • K N Kiran ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author
  • A Singh ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author https://orcid.org/0009-0006-0250-6218
  • S K Singh ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author
  • O P Awasthi ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author
  • P Yadav ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author
  • K B Sandeep ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India Author

DOI:

https://doi.org/10.24154/jhs.v19i1.2417

Abstract

Induction of tetraploidy in citrus is commonly meant for the development of triploid seedless cultivars as well as resistance against abiotic and biotic stresses. Three-year-old, 20 second-generation colchicine treated (0.05, 0.10, 0.15 and 0.20%) plants (colchiploids), established from the putative tetraploid branches of the first generation colchiploids of sweet orange (Citrus sinensis (L.) Osbeck) cv. Mosambi vegetatively propagated on Jatti khatti rootstock, along with their wild (parent) type, were characterized based on morphological and physiological traits. Plant height and canopy volume were reduced, but stem girth, nodes per shoot and bark: wood increased in the majority of the second-generation colchiploids related to the wild type. Colchiploids also possessed improved flower characteristics in terms of length and width. The stomatal dimensions increased, but stomatal concentration reduced in all the colchiploids. Colchicine treatment also caused significant variations in leaf gas exchange parameters, including photosynthetic rate, intercellular CO2 concentration, leaf net transpiration rates, stomatal conductance, and intrinsic water use efficiency in colchiploids affecting their photosynthetic activities. The solid tetraploids identified on the basis of morpho-physiological characterization can be used in future breeding programmes for the development of triploid seedless citrus cultivars or can be used for the mitigation of biotic and abiotic stresses.

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Author Biographies

  • K N Kiran, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Ph.D. Scholar, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

  • A Singh, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Principal Scientist, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

  • S K Singh, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Principal Scientist  and Director, Division of Fruits crops ICAR-Indian Institute of Horticultural Research, Bengaluru 560089 

  • O P Awasthi, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Principal Scientist & Head, Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110012

  • P Yadav, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Senior Scientist, Division of genetics ICAR-Indian Agricultural Research Institute, New Delhi 110012

  • K B Sandeep, ICAR-Indian Agricultural Research Institute, New Delhi - 110012, India

    Division of Fruits and Horticultural Technology, ICAR-Indian Agricultural Research Institute, New Delhi 110012

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Published

10-06-2024

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Original Research Papers

How to Cite

Kiran, K. N., Singh, A., Singh, S. K., Awasthi, O. P., Yadav, P., & Sandeep, K. B. (2024). Morpho-physiological characterization of second generation colchiploids in sweet orange (Citrus sinensis (L.) Osbeck) cv. Mosambi. Journal of Horticultural Sciences, 19(1). https://doi.org/10.24154/jhs.v19i1.2417