Synergistic Use of Hypocotyl Explants and High Bap Preconditioning for Enhanced Transformation Frequency in Brinjal (Solanum melongena L.)
DOI:
https://doi.org/10.24154/jhs.v1i2.650Keywords:
Solanum melongena, Transformation, Hypocotyl, BAP, PreconditioningAbstract
Poor regeneration is one of the limiting factors in the development of transgenic crops since Agrobacterium as a plant pathogen can disturb the fragile in vitro conditions with wounding and infection regimes. We have tried to optimize the transformation system in two important varieties of brinjal after Agrobacterium infection to the explants. The effect of explant was studied and hypocotyls were found to be better than cotyledonary leaves. High BAP during the preconditioning period was found to further enhance the regeneration rate. Therefore, use of hypocotyls and high BAP during preconditioning can improve the regeneration of transformed cells and recovery of transformants in vegetables especially brinjal.
References
Allichio, R., Del Grosso, E . and Boschieri, E. 1982. Tissue cultures and plant regeneration from different explants in six cultivars of Solanum melongena. Experientia, 38: 449-450.
Billings, S., Jelenkovic, G., Chin, C. K. and Eberhardt, J.1997. The effect of growth regulation and antibiotics on eggplant transformation. J. Amer. Soc. Hort. Sci.,122:158-162.
Collonnier, C., Fock, I., Kashyap, V., Rotino, G. L., Daunay,M. C., Lian, Y., Mariska, I. K., Rajam, M. V., Servaes,A., Ducreux, G. and Sihachakr, D. 2001, Applications of biotechnology in eggplant. Plant Cell Tissue OrganCult.,5: 91-107.
Ditt, R. F., Nester, E. W. and Comai, L. 2001. Plant gene expression response to Agrobacterium tumefaciens. Proc. Natl. Acad. Sci.,USA, 98: 10954-10959.
Freitas, V. G., Lacorte, C., Sachetto-Martins, G., Krul, W.R., Oliveira, D. E., Neves, L. J. and Mansur, E. 1997. Identification of competent cells for Agrobacterium-transformation and in vitro regeneration in peanut leaf and cotyledon explants. R. Bras. Fisiol. Veg.,9:157-167.
Gelvin, S. B. 2000. Agrobacterium and plant genes involved in T-DNA transfer and integration. Annu. Rev. Plant.Physiol. Plant. Mol. Biol.,51: 223-256.
Hamza, S. and Chupeau, Y. 1993. Re-evaluation of conditions for plant regeneration and Agrobacterium-mediated transformation from tomato. J. Exp. Bot.,44: 1837-1845.
Hanur, V. S. 2004. Genetic transformation of human cells by a soil phytopathogen presents common molecular strategies. Curr. Sci.,87: 856-857.
Kumar, P. A., Mandaokar, A. D. and Sharma, R. P. 1998a.Genetic engineering for the improvement of eggplant (Solanummelongena L.). Ag Biotech News Inform.,10: 329-332.
Kumar, P. A., Mandaokar, A., Sreenivasu, K., Chakrabarti,S. K., Bisaria, S., Sharma, S. R., Kaur, S. and Sharma,R. P. 1998b. Insect-resistant transgenic brinjal plants.Mol. Breed.,4: 33-37.
Kumar, S. V. and Rajam, M. V. 2005. Enhanced induction of vir genes results in the improvement of Agrobacte-rium-mediated transformation of eggplant. J. Plant Biochem. Biotechnol.,14: 89-94.
Lin, J. J., Assad-Garcia, N. and Kuo, J. 1994. Improved regeneration of plant tissues: a novel medium format and membrane based liquid culture system. Focus,16:72.
Lipp-Joao, K. H. and Brown, T. A. 1993. Enhanced transformation of tomato co-cultivated with Agrobacterium tumefaciens C58C1 Rifr::pGSFR1161 in the presence of acetosyringone. Pl. Cell Rep.,12:422-425.
Magioli, C. and Mansur, E. 2005. Eggplant (Solanummelongena L.): tissue culture, genetic transformation and use as an alternative model plant. Acta Bot. Bras.,19: 139-148.
Magioli, C., Rocha, A. P. M., de Oliveira, D. E. and Mansur,E. 1998. Efficient shoot organogenesis of eggplant (Solanummelongena L.) induced by thidiazuron. Pl.Cell Rep.,17: 661-663.
Mc-Hughen, A., Jordan, M. and Feist, G. 1989. A precultureperiod prior to Agrobacterium inoculation increases production of transgenic plants. J. Plant Physiol.,135: 245-248.
Murashige, T. and Skoog, F. 1962. A revised method for rapid growth and bioassays with tissue cultures.Physio.l Plant.,15: 473-497.
Robinette, D. and Matthysse, A. G. 1990. Inhibition by Agrobacterium tumefaciens and Pseudomonas savastanoi of development of the hyper sensitive response elicited by Pseudomonas syringae pv.phaseolicola. J. Bacteriol.,172: 5742-5749.
Rotino, G. L. and Gleddie, S. 1990. Transformation of eggplant (Solanum melongena L.) using a binary Agrobacterium tumefaciens vectors. Plant Cell Rep.,9: 26-29.
Sharma, P. and Rajam, M. V. 1995. Genotype, explant and position effects on organogenesis and embryogenesis in eggplant (Solanum melongena L.). J. Exp. Bot,,46: 135-141.
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Copyright (c) 2006 Vageeshbabu S Hanur, D P Prakash, B S Deepali, R Asokan, Y L Ramachandra, Riaz Mahmood, Lalitha Anand (Author)
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