In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

A A Waman; P Bohra; R Karthika Devi; J Pixy

doi:10.24154/jhs.v16i1.1101

Authors

A A Waman ICAR-Central Island Agricultural Research Institute Author
P Bohra ICAR-Central Island Agricultural Research Institute Author
R Karthika Devi ICAR-Central Island Agricultural Research Institute Author
J Pixy ICAR-Central Island Agricultural Research Institute Author

DOI:

https://doi.org/10.24154/jhs.v16i1.1101

Keywords:

Ex vitro rooting, Glucose, Hardening, Inoculum size, Mango ginger, Tropical

Abstract

Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.

Downloads

Download data is not yet available.

References

Aggarwal, B.B., Sundaram, C., Malani, N. and Ichikawa, H.2007. Curcumin: the Indian solid gold. Adv. Exp. Med. Biol. 595:1-75.

Bairu, M.W., Stirk, W.A., Dolezal, K. and Staden, J.V. 2008. The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp.AAA). Plant Cell Tiss. Org. Cult.95:373-379.

Bohra, P., Waman, A.A., Sathyanarayana, B.N. and Umesha, K. 2016. Concurrent ex vitro rooting and hardening in Ney Poovan banana (Musa AB): effect of carbon sources and their interactions. Erwerbs-Obstbau 58:193-198.

Capellades, M., Lemeur, R. and Debergh, P. 1991. Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell, Tissue and Organ Culture, 25(1): 21-26.

Elboullani, R., Lagram, K., Al Mousadik, A. and Serghini, M.A. 2017. Effect of explant density and size on the in vitro proliferation and growth of separated shoots of globe artichoke (Cynara cardunculus var. scolymus L.). J. Mat. Environ. Sci.8:2469-2473.

El-Hawaz, R.F., Bridges, W.C. and Adelberg, J.W. 2015. In vitro growth of Curcuma longa L. in response to five mineral elements and plant density in fed-batch culture systems. PLoS One10:e0118912.

Escalona, M., Cejas, I., Gonzalez-Olmedo, J., Capote, I., Roels, S., Canal, M.J., Rodriguez, R., Sandoval, J. and Debergh, P. 2003. The effect of meta-topolin on plantain propagation using a temporary immersion bioreactor.InfoMusa12:28-30.

Hutami, S. and Purnamaningsih, R. 2003. Augment clonal Appointment Mangga (Curcuma mangga) through cultured in vitro. Bulletin Plasma Nutfah 9: 39-44.

Leong-Škorničková, J., Otakar, Š. and Karol, M. 2010. Back to types! Towards stability of names in Indian Curcuma L. (Zingiberaceae). Taxon 59:269–282.

Liu, Y.and Nair, M.G. 2012.Curcuma longa and Curcuma mangga leaves exhibit functional food property. Food Chemistry135:634–640.

Lichtenthaler, H. K., Wellburn, A. 1983. Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 603: 591-592.

Madhulatha P., Kirubakaran S.I., Sakthivel N. 2006. Effects of carbon sources and auxins on in vitro propagation of banana. Biol. Plant. 50: 782–784

Malek, S.N.A., Lee, G.S., Hong, S.L., Yaacob, H., Wahab, N.A., Weber, J.F.and Shah, S.A.A. 2011. Phytochemical and cytotoxic investigations of Curcuma mangga rhizomes. Molecules 16:4539–4548.

Pikulthong, V., Teerakathiti, T., Thamchaipenet, A. and Peyachoknagul, S. 2016. Development of somatic embryos for genetic transformation in Curcuma longa L. and Curcuma mangga Valeton & Zijp. Agric. Nat. Resour. 50:276-285.

Raihana, R., Faridah, Q.Z., Julia, A.A., Abdelmageed, A.H.A. and Kadir, M. 2011.In vitro culture of Curcuma mangga from rhizome bud. J. Med. Plants Res. 5:6418-6422.

Ranaweera, K.K., Gunasekara, M.T.K. and Eeswara, J.P. 2013. Ex vitro rooting: A low cost micropropagation technique for Tea (Camellia sinensis (L.) O. Kuntz) hybrids. Sci. Hort. 155:8-14.

Salvi, N., George, L. and Eapen, S. 2001. Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss. Org. Cult. 66: 113-119.

Sharmin, S.A., Alam, M.J., Sheikh, M.M.I., Zaman, R., Khalekuzzaman, M., Mondal, S.C., Haque, M.A., Alam, M.F. and Alam, I. 2013. Micropropagation and antimicrobial activity of Curcuma aromatica Salisb., a threatened aromatic medicinal plant. Turkish J. Biol. 37:698-708.

Singh, A.K. 2017. Revisiting the status of cultivated plant species agrobiodiversity in India: an overview. Proc. Indian Nat. Sci. Acad. 83:151–174.

Sirirugsa, P., Larsen, K. and Maknoi, C.2007.The genus Curcuma L. (Zingiberaceae): distribution and classification with reference to species diversity in Thailand. Gardens’ Bull. Singapore 59:203–220.

Suthasinee, S. and Supinya, T. 2019. Antiinûammatory and wound healing eûects of cream containing Curcuma mangga extract. J.Ethnopharmacol.238:111828.

Wahab, I.R.A., Blagojevic, P.D., Radulovic, N.S. and Boylan, F. 2011. Volatiles of Curcuma mangga Val. & Zijp (Zingiberaceae) from Malaysia. Chem. Biodiv. 8: 2005–2014.

Waman, A.A., Bohra, P. and Aarthi, S. 2018. Propagule size affects yield and quality of Curcuma mangga Val. et Zijp.: An important medicinal spice. Ind. Crops Prod.124: 36-43.

Waman, A.A. and Bohra, P. 2018. Factors governing success in shoot tip culture of bananas with special reference to mixed genomic groups: an overview. Erwerbs-Obstbau 61:9-21.

Woztania A. 2010. Effect of meta-topolin on in vitro propagation of Pelargonium x hortorum and Pelargonium x hederaefolium cultivars. Acta Societatis Botanicorum Poloniae 79: 101-106.