Effect of Seed Disinfectants on in vitro Seed Germination and Seedling Development in Eggplant
DOI:
https://doi.org/10.24154/jhs.v9i1.224Keywords:
Seed Germination, Seedling Growth, Culture Contamination, Bleach, Mercuric Chloride, Sodium HypochloriteAbstract
The present investigation reports effects of disinfectants on culture contamination, seed germination and seedling growth in eggplant. Mercuric chloride hampers seed germination when seeds are treated with 0.1% solution above 2 min duration and lesser durations are not helpful in controlling in vitro contamination. The highest seed germination (89.97%) was recorded with 50% commercial bleach for 20 min in the genotype BL-3, followed by BR-16 (88.53%), BR-14 (86.19%), BL-5 (86.16%) and BSR-23 (85.57%). However, least number of seeds germinated in BR-18 (10.94%), BSR-25 (13.27%) and BL-7 (18.62%) when disinfected with 75% commercial bleach for 25min. Overall results revealed that 50% commercial bleach concentration (73.76%) was better than 75% (36.85%), and 20 min duration (60.82%) was better than 25 min (49.80%) for seed disinfection. Among the varieties, BL-3 was at the top (68.85%) and BR-18 at the lowest (34.16%) edge for per cent seed germination. Seedling growth (cm) with use of commercial bleach was quite satisfactory compared to disinfection with HgCl2, where, 50% commercial bleach favored a good stand of plant lets even after 10 days, showing a healthy root (3cm), hypocotyl (5cm) and cotyledons (1.5cm).
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Aref, A. and Baki, A. 1974. Pitfalls in using sodium hypochlorite as a seed disinfectant in 14c incorporation studies. Plant Physiol., 53:768-771
Cheema, H.S. and Singh, B. 1990. A user’s manual to CPCS-1. A Computer Programme Package for the Analysis of Commonly used Experimental Designs, pp-1, PAU, Ludhiana, Punjab, India
Habib, A., Sahi, S.T., Ghazanfar, M.U. and Ali, S. 2007. Location of seed-borne mycoflora of eggplant (Solanum melongena L.) in different seed components and impact on seed germinability. Int’l. J. Agril. Biol., 9:514–516
Ho, C.K., Jacobs, G. and Donald, D.G.M. 1995. Effects of sodium hypochlorite, ethanol and culture medium on seed germination of Paulownia species. Seed Sci.Tech., 23:157-163
Kaur, M., Dhatt, A.S., Sandhu, J.S., Sidhu, A.S. and Gosal, S.S. 2011a. Role of genotype, explant and growth harmones on regeneration in eggplant (Solanum melongena). Indian. J. Agri. Sci., 81:38-43
Kaur, M., Dhatt, A.S., Sandhu, J.S. and Gossal, S.S. 2011b. In vitro plant regeneration in brinjal from cultured seedling e plant. Indian J. Hort., 68:61-65
Ling, T., Fangke, Y. and Jun, R. 2010. Effect of mercury to seed germination, coleoptile growth and root elongation of four vegetables. Res. J. Phytochem., 4:225-233
Mir, K.A., Dhatt, A.S., Sandhu, J.S. and Gosal, S.S. 2008. Genotype, explant and culture medium effects on somatic embryogenesis in eggplant (Solanum melongena L.). Hort. Environ. Biotechnol., 49:182-187
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497
Mustafa, 2002. The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax (Linum usitatissimum). Naturwissenschaften, 89:259-261
Pernezny, K., Nagata, R., Raid, R.N., Collins, J. and Carroll, A. 2002. Investigation of seed treatments for management of bacterial leaf spot of lettuce. Plant Dis., 86:151-55
Rick, C.M. and Borgnino, F.H. 1989. A method for improving seed germination of solanaceous species. http://tgrc.ucdavis.edu/seed_germ.html
Sarker, R.H., Sabina, Y. and Hoque, M.I. 2006. Multiple shoot formation in eggplant (Solanum melongena L.). Pl. Tiss. Cult. Biotech., 16:53-61
Wenny, D.L. and Dumroese, R.K. 1987. Germination of conifer seeds surface-sterilized with bleach. Tree Planters’ Notes, 38:18-21
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Copyright (c) 2014 M Kaur, A S Dhatt, A S Sidhu (Author)
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