Microsatellite Identification in Solanaceae Crops Associated with Nucleoside Diphosphate Kinase (NDK) Specific to Abiotic Stress Tolerance through in silico Analysis
DOI:
https://doi.org/10.24154/jhs.v8i2.300Keywords:
Nucleoside Diphosphate Kinase (NDK), Microsatellite, Abiotic Stress, SolanaceaeAbstract
Abiotic stress often causes a series of morphological, physiological, biochemical and molecular changes that affect plant growth, development and productivity. To cope with abiotic stresses, it is necessary to understand plant responses to stresses that disturb homeostatic equilibrium at the cellular and molecular level. Genomic information on Capsicum annuum has been explored to identify microsatellite markers associated with abiotic stress tolerance and assign them to cognate functional groups related to specific stress responses. Several in silico methods have been used to identify simple sequence repeats associated with stress responsive gene candidates in Capsicum annuum. In this study, a microsatellite marker has been identified in Capsicum annuum associated with Nucleoside Diphosphate Kinase (NDK) having multiple environmental stress tolerance (oxidative, high temperature and salt stress) and which is also highly conserved in crops of Solanaceae. These are house-keeping enzymes that maintain intracellular levels of all nucleoside triphosphates (NTP) with the exception of adenosine triphosphate (ATP). These are also involved in phytochrome A response, UV-B signaling, auxin responses and oxidative stress signaling.
Downloads
References
Bowler, C. and Fluhr, R. 2000. The role of calcium and activated oxygen as signals for controlling cross- tolerance. Trends Pl. Sci., 5:241–246
Boyer, J.S. 1982. Plant productivity and environment. Science, 218:443–448
Foyer, C.H., Descourvieres, P. and Kunert, K.J. 1994. Protection against oxygen radicals: An important defense mechanism studied in transgenic plants. Plant Cell Environ., 17:507-523
Im, Y.J., Kim, J.I., Shen, Y., Na, Y., Han, Y.J., Kim, S.H., Song, P.S. and Eom, S.H. 2004. Structural analysis of Arabidopsis thaliana nucleoside diphosphate kinase- 2 for phytochrome-mediated light signaling. J. Mol. Biol., 343:659-70
Moon, H., Lee, B., Choi, G., Shin, D., Prasad, D.T., Lee, O., Kwak, S.S., Kim, D.H., Nam, J., Bahk, J., Hong, J.C., Lee, S.Y., Cho, M.J., Lim, C.O. and Yun, D.J. 2003. NDP kinase 2 interacts with two oxidative stress-activated MAPKs to regulate cellular redox state and enhances multiple stress tolerance in transgenic plants. Proc. Nat’l. Acad. Sci., USA,
:358–363
Parks, R.E.J. and Agarwal, R.P. 1973. Nucleoside diphosphokinases. In: The Enzymes, Boyer, P.D (Ed.). Academic, New York, USA, pp. 307–334
Yang, K.A., Moon, H.J., Kim, G.T., Lim, C.J., Hong, J.C., Lim, C.O. and Yun, D.J. 2003. NDP kinase 2 regulates expression of antioxidant genes in Arabidopsis. Proc.Jpn. Acad. Ser., B, 79:86–91
Yano, A., Umeda, M. and Uchimiya, H. 1995. Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ related alteration of NDK activities during rice seed germination (Oryza sativa L.). Pl. Mol. Biol., 27:1053–1058
Downloads
Published
Issue
Section
License
Copyright (c) 2013 Reena Rosy Thomas, M K Chandra Prakash, M Krishna Reddy, Sukhada Mohandas, Riaz Mahmood (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
