Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

Authors

  • D P Prakash Author
  • B S Deepali Author
  • R Asokan Author
  • Y L Ramachandra Author
  • Lalitha Anand Author
  • Vageeshbabu S Hanur Author

DOI:

https://doi.org/10.24154/jhs.v2i2.612

Keywords:

Growth Regulators, Explant, Brinjal, Transformation

Abstract

Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.

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Published

31-12-2007

Issue

Section

Original Research Papers

How to Cite

Prakash, D. P., Deepali, B. S., Asokan, R., Ramachandra, Y. L., Anand, L., & Hanur, V. S. (2007). Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota. Journal of Horticultural Sciences, 2(2), 94-98. https://doi.org/10.24154/jhs.v2i2.612

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