Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota

  • D P Prakash
  • B S Deepali
  • R Asokan
  • Y L Ramachandra
  • Lalitha Anand
  • Vageeshbabu S Hanur
Keywords: Growth Regulators, Explant, Brinjal, Transformation

Abstract

Effects of growth regulators and type of explants on transformation and in vitro morphogenetic responses of brinjal cv. Manjarigota were studied. Both hypocotyl and cotyledonary explants showed marked influence on in vitro morphogenetic responses after Agrobacterium co-cultivation. Hypocotyl explants showed callus initiation and regeneration responses earlier than cotyledonary leaves. Hypocotyl explants were found to be better than cotyledonary leaf explants in regenerating shoots after Agrobacterium co-cultivation. There was delay and reduction in both callus and regeneration responses in Agrobacterium co-cultivated explants. Hypocotyl explants showed the highest regeneration response on MS medium containing 2 μM BAP and 0.05 μM NAA while cotyledonary leaves did not show regeneration response after Agrobacterium co-cultivation. However, they showed green buds on MS medium containing 10 μM BAP and 1 μM NAA, which could not differentiate into shoots. Overall, hypocotyl explants were found better in regenerating shoots after Agrobacterium co-cultivation.

Published
2018-12-31
How to Cite
Prakash, D., Deepali, B., Asokan, R., Ramachandra, Y., Anand, L., & Hanur, V. (2018). Effects of Growth Regulators and Explant-Type on Agrobacterium-Mediated Transformation in Brinjal (Solanum melongena L.) cv. Manjarigota. Journal of Horticultural Sciences, 2(2), 94-98. Retrieved from https://jhs.iihr.res.in/index.php/jhs/article/view/612
Section
Articles

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