Exploring pollen cryopreservation, germination, and morphometry ofmango (Mangifera indica L.) Appemidi genotypes
DOI:
https://doi.org/10.24154/jhs.v20i2.2917Keywords:
Appemidi, cryopreservation, pollen germination, genotype, diversityAbstract
Pollen cryopreservation is a vital technique for maintaining genetic diversity in highly heterozygous crops like mango, ensuring the long-term sustainability of crop improvement programs. The present study examined the effects of cryopreservation of pollen from mango Appemidi genotypes, to enhance and protect the mango gene pool. Dehisced and near-dehisced anthers were collected during the optimal time frame of 10:30–11:30 am. In vitro pollen germination assays revealed significant variability among genotypes, with germination ranging from 10.84% to 85.46%. Notably, the genotype Arenuru exhibited the highest pollen germination rate and greatest pollen tube elongation after one month of cryopreservation. Further, scanning electron microscopy, revealed significant structural variations among the accessions. Overall, this study highlights the efficacy of pollen cryopreservation as a viable strategy for conserving genetic diversity and its potential contribution to future mango breeding and improvement programs.
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