16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots

Authors

  • D Girija Author
  • P K Rajeevan Author
  • Swathi Balakrishnan Author
  • P S Panchami Author
  • Mahesh Mohan Author

DOI:

https://doi.org/10.24154/jhs.v13i1.54

Keywords:

Endophyte, orchid, diversity

Abstract

Orchids are one of the main groups of ornamental plants commercially exploited. In the present study, we analyzed the diversity of bacterial community in Phalaenopsis root using metagenomic approach. The diversity of bacterial taxonomic category was assessed at different Operational Taxonomic Unit (OTU) levels using Ribosomal Database Project (RDP) pipeline and MG-RAST. At phylum level, Proteobacteria (61.34%) was the most dominant group followed by unclassified derived from bacteria (24.74%) and Actinobacteria (12.52%). Genus level analysis revealed the abundance of Rubrobacter, Pseudomonas and Acinetobacter. The study revealed that of the total species detected 50.83 per cent were unclassified, stressing the importance of metagenomics to assess the diversity of endophytes associated with orchid roots.

Downloads

Download data is not yet available.

References

Bulgarelli D., Schlaeppi, K., Spaepen, S., Ver Loren and van Themaat E. 2013. Structure and functions of the bacterial microbiota of plants. Annu. Rev. Plant Biol, 64: 807-838. DOI: https://doi.org/10.1146/annurev-arplant-050312-120106

Hallmann, J., Berg, G. and Schulz, B. 2006. Isolation procedures for endophytic microorganisms.in: B.E. Schulz, C.C. Boyle, T. Sieber (Eds.), Microbial Root Endophytes, Vol. 9, Springer, Berlin Heidelberg 2006, p. 299–319. DOI: https://doi.org/10.1007/3-540-33526-9_17

Issa, O.M., Defarge, C., Le Bissonnais, Y., Marin, B., Duval, O., Bruand, A., D’Acqui, L.P. Nordenberg, S. and Annerman, M., 2007. Effects of the inoculation of cyanobacteria on the microstructure and the structural stability of a tropical soil. Plant Soil, 290:209–219 DOI: https://doi.org/10.1007/s11104-006-9153-9

Lozupone, C.A. and Knight, R. Global patterns in bacterial diversity. 2007. Proc. Natl. Acad. Sci.,104: 11436–11440. DOI: https://doi.org/10.1073/pnas.0611525104

Spiers, A.J., Buckling, A. and Raineythen, P.B. 2000.The causes of Pseudomonas diversity. Microbiology,146:2345–2350. DOI: https://doi.org/10.1099/00221287-146-10-2345

Teixeira da Silva J.A., Tsavkelova, E.A., Zeng, S., Parthibha n, S. and Rao M. V. 2015. Symbiotic in vitro seed propagation DOI: https://doi.org/10.1007/s00299-015-1829-2

of Dendrobium: fungal and bacterial partners and their influence on plant growth and development. Planta, 24:21–22.

Trivedi, P. Spann, T. and Wang, N. 2011. Isolation and characterization of beneficial bacteria associated with citrus roots in Florida Microb Ecol., 62:324-336 DOI: https://doi.org/10.1007/s00248-011-9822-y

Tsavkelova, E.A., Klimova, Y.S., Cherdyntseva, T.A. and Netrusov, A.I.2006. Microbial producers of plant growth stimulators and their pr actical use: a r eview, Appl. Biochem.Microbiol., 42:133–143. DOI: https://doi.org/10.1134/S0003683806020013

Yang, R., Liu, P. and Ye, W. 2017. Illumina-based analysis of endophytic bacterial diversity of tree peony (Paeonia Sect. Moutan) roots and leaves. Braz.J.Microbiol.,48: 695-705. DOI: https://doi.org/10.1016/j.bjm.2017.02.009

Wilkinson, K., Dixon, K., Sivasithamparam, K. and Ghisalberti, E., 1994. Effect of IAA on symbiotic germinationof an Australian orchid and its production by orchid-associated bacteria. Plant Soil, 159: 291–295. DOI: https://doi.org/10.1007/BF00009292

Downloads

Published

30-06-2018

Issue

Section

Research Notes

How to Cite

Girija, D., Rajeevan, P. K., Balakrishnan, S., Panchami, P. S., & Mohan, M. (2018). 16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots. Journal of Horticultural Sciences, 13(1), 103-107. https://doi.org/10.24154/jhs.v13i1.54

Similar Articles

1-10 of 68

You may also start an advanced similarity search for this article.