16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots

  • D Girija
  • P K Rajeevan
  • Swathi Balakrishnan
  • P S Panchami
  • Mahesh Mohan
Keywords: Endophyte, orchid, diversity

Abstract

Orchids are one of the main groups of ornamental plants commercially exploited. In the present study, we analyzed the diversity of bacterial community in Phalaenopsis root using metagenomic approach. The diversity of bacterial taxonomic category was assessed at different Operational Taxonomic Unit (OTU) levels using Ribosomal Database Project (RDP) pipeline and MG-RAST. At phylum level, Proteobacteria (61.34%) was the most dominant group followed by unclassified derived from bacteria (24.74%) and Actinobacteria (12.52%). Genus level analysis revealed the abundance of Rubrobacter, Pseudomonas and Acinetobacter. The study revealed that of the total species detected 50.83 per cent were unclassified, stressing the importance of metagenomics to assess the diversity of endophytes associated with orchid roots.

Published
2018-06-30
How to Cite
Girija, D., Rajeevan, P. K., Balakrishnan, S., Panchami, P. S., & Mohan, M. (2018). 16S rRNA gene taxonomic profiling of endophytic bacteria associated with phylaenopsis roots. Journal of Horticultural Sciences, 13(1), 103-107. https://doi.org/10.24154/JHS.2018.v13i01.012
Section
Short Communications