Assessment of Genetic Diversity in Guava (Psidium guajava) Germplasm Using Microsatellites
DOI:
https://doi.org/10.24154/jhs.v9i2.180Keywords:
Guava, Genetic Diversity, Dendrogram, Simple Sequence RepeatsAbstract
Although the varietal diversity is fairly rich in guava, most varieties lack one or more desirable characters. Hence, attempts were made for improving specific traits, viz., attractive pink pulp colour, soft seeds, medium fruit size, high TSS and high ascorbic acid. Genetic diversity analysis is a prerequisite for identifying potential parents in breeding programs and germplasm conservation. Molecular characterization helps discriminate closely-related genotypes, as, this technique is unaffected by environment, rendering it more reliable. In this study, 48 polymorphic SSRs screened from a total of 115 SSR markers were used for analyzing marker segregation in 72 guava accessions. Statistical analysis was done using IDENTITY1.0 and CERVUS 3.0 software. Cluster analysis was done with DARwin 5.0 software, using Wards Minimum Variance method, and weighted group neighbour joining method, to check reliability of grouping among clusters. The trend in grouping was found to be similar in both methods. Dendrograms generated showed that the hybrids clustered with their parents; exotic collections fell into two different sub-groups based on productivity; the wild species formed one group; and Navalar cultivars from Dharwad clustered together, reflecting similar origin.
References
Cholastova, T. and Knotova, D. 2012. Using morphological and microsatellite (SSR) markers to assess the genetic diversity in alfalfa (Medicago sativa L.). Int’l. J. Biol. Vet. Agril. Food Engg., 6:146-152
Dinesh, M.R. and Vasugi, C. 2010. Guava improvement in India and future needs. J. Hortl. Sci., 5:94-108
Doyle, J.J. and Doyle, J.L. 1990. Isolation of plant DNA from fresh tissue. Focus, 12:13-15
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39:783-791
Hadadinejad, M., Ebadi, A., Naghavi, M.R. and Nikkhah, R. 2011. Genealogy and molecular diversity of Iranian grapevine progenies. J. Agril. Sci. Tech., 13:1147-1161
Hayes, W.B. 1953. Fruits growing in India. Kitabistan, Allahabad, India
Johnson, A.R. and Wichern, D.W. 1992. Applied multivariate statistical analysis. 3rd edition, Prentice-Hall, Englewood Cliffs, NJ, USA
Joseph, B. and Mini Priya, R. 2011. Review on nutritional, medicinal, pharmacological properties of guava (Psidium guajava L.). Int’l. J. Pharm. Biosci., 2:54-69
Kalinowski, S.T., Taper, M.L. and Marshall, T.C. 2007. Revising how the computer program Cervus accommodates genotyping error increases success in paternity assignment. Mol. Ecol., 16:1099-1106
Kanupriya, Madhavi Latha, P., Aswath C., Reddy, L., Padmakar, B., Vasugi, C. and Dinesh, M.R. 2011. Cultivar identification and genetic fingerprinting of guava (Psidium guajava L.) using microsatellite markers. Int’l. J. Fruit Sci., 11:184–196
Karp, A., Kresovich, S., Bhat, K.V., Ayad, W.G. and Hodgkin, T. 1997. Molecular tools in plant genetic resources conservation: A guide to the technologies IPGRI, Rome
Lakshmana Reddy, D. C., Kiran, K., Srinivas Reddy, S.H., Kanupriya, Aswath, C. and Singh, T.H. 2012. SSR- based DNA barcodes as a tool for identification of eggplant genotypes. Int’l. J. Veg. Sci., 18:260-271
Lessa, E.P. 1990. Multidimensional analysis of geographic genetic structure. Sys. Biol., 39:242-252
Mahajan, R., Tabia, S., Raina, G. and Mangotra, N. 2012. Assessment of genetic diversity of non-basmati rice of Jammu and Kashmir using microsatellite markers. J. Cereals and Oil Seeds, 3:21-27
Mondini, L., Noorani, A. and Pagnotta, M.A. 2009. Assessing plant genetic diversity by molecular tools. Diversity, 1:19-35
Pathak, R.K. and Ojha, C.M. 1993. Genetic resources of guava. Adv. Hort. Fruit Crops., 1:143-147
Perrier, X., Flori, A. and Bonnot, F. 2003.Genetic diversity of cultivated tropical plants (ed. P. Hamon). Data Analysis Methods, 43–76
Rajkumar, G., Weerasena, J., Fernando, K. and Liyanage, A. 2011. Assessment of genetic diversity among Sri Lankan rice varieties by AFLP markers. Pl. Genetic Resources, 9:224-228
Risterucci, A.M., Duval, M.F., Rohde, W. and Billotte, N. 2005. Isolation and characterization of microsatellite loci from Psidium guajava L. Mol. Ecol. Notes, 5:745-748
Rodriguez, N.N., Valdés-Infante, J., Becker, D., Velázquez, B., Coto, O., Ritter, E. and Rohde. 2004. Morphological, agronomic and molecular characterization of Cuban accessions of guava (Psidium guajava L.). J. Genet. Breed., 8:70-90
Swofford, D.L., Olsen, G.J., Waddell, P.J. and Hillis, D.M. 1996. Phylogenetic inference. In: D.M. Hillis et al. (eds.) Molecular Systematics, 2nd edition, Sinauer Associates, Sunderland, MA., USA, pp. 407–514
Vasugi, C. and Rami Reddy, P.V. 2003. Chacterization and evaluation of some exotic guava accessions under Bangalore conditions. Indian J. Pl. Genet. Resources, 17:189-192
Vasugi, C. and Dinesh, M.R. 2007. Genetic variability in some Psidium species. Indian J. Agril. Sci., 77:420-423
Wagner, H.W. and Sefc, K.M. 1999. Identity 1.0, Centre for Applied Genetics, University of Agricultural Sciences, Vienna, Austria
Ward, J.H. Jr.1963. Hierarchical grouping to optimize an objective function. J. Amer. Statistical Assoc., 58:236–244
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Copyright (c) 2014 M V Naga Chaithanya, M R Dinesh, C Vasugi, D C Lakshmana Reddy, D Sailaja, C Aswath (Author)
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