Molecular Diversity Analysis in F3 Intergeneric Population of Papaya (Carica papaya L.)
DOI:
https://doi.org/10.24154/jhs.v9i1.206Keywords:
Carica papaya, Vasconcellea cauliflora, Intergeneric Hybridization, Molecular DiversityAbstract
Attempts were made to estimate molecular diversity present in F3 populations of intergeneric crosses between Carica papaya L. (Var. Pusa Nanha and CP 50) and Vasconcellea cauliflora. Molecular studies revealed that PCR amplification using five ISSR primers in 40 F3 progenies yielded 53 reproducible amplified bands. Of the 53 bands, 44 were polymorphic (83.02%). Polymorphic Information Content (PIC) value ranged between 0.90 (ISSR 807 x 810) and 0.66 (ISSR 834 x 810). Similarity coefficients based on five ISSR markers ranged from 0.05 to 0.96. Maximum similarity was observed for genotypes 1, 4 and 6 of Pusa Nanha x Vasconcellea cauliflora (0.96). Minimum similarity was observed between genotypes 3 and 14 of CP 50 x Vasconcellea cauliflora (0.04). This higher genetic diversity of papaya progenies stands to contribute to development of new varieties and, using the data, further hybridization and selection can be planned.
References
Aitchitt, M., Ainsworth, C.C. and Thangavelu, M. 1993. A rapid and efficient method for the extraction of total DNA from mature leaves of the date palm (Phoenix dactylifera L.). Pl. Mol. Biol. Repr., 11:317-319
Bajpai, A., Srivastava, N., Rajan, S. and Chandra, R. 2008. Genetic diversity and discrimination of mango accessions using RAPD and ISSR markers. Indian J. Hort., 65:377-382
Benbouza, H., Jacquemin, J.M., Baudoin, J.P. and Mergeai, G. 2006. Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. Biotechnol. Agron. Soc. Environ., 10:77-81
Chan, Y.K. 2004. Field performance of papaya lines selected for tolerance to ring spot virus disease. J. Trop. Agri. Fd. Sci., 31:128-137
Dhanam, S. 2006. Studies on papaya ring spot disease. M.Sc. (Plant Pathology) Thesis, Tamil Nadu Agricultural University, Coimbatore
Horovitz, S. and Jimenez, H. 1967. Cruzameintos interspecificos intergenericos en Caricaceas ysus implcaciones fitoecnicas. Agron. Trop., 17:323-343
Jaccard, P. 1908. Nouvelles rescerches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat., 44:233-270
Khuspe, S.S., Hendre, R.R., Mascarenhas, A.F., Jaganathan, V., Thombre, M.V. and Joshi, A.B. 1980. Utilization of tissue culture to isolate intergeneric hybrids in Carica L. In: Rao, P.S., Heble, M.R. and Chadla, M.S. (eds). Plant tissue culture, genetic manipulation and somatic hybridization of plant cells. Bhabha Atomic Research Centre, Trombay, Bombay, India, pp.198-205
Levi, F.R. and Roland, G.W. 1997. Versatile tools used in plant breeding programme by using ISSR markers. Agronomia-esoamericana, 8:121-125
Mignouna, H.D., Ikca, N.Q. and Thottapilly, G. 1998. Genetic diversity in cowpea as revealed by random amplified polymorphic DNA. J. Genet. Breed., 52:151-159
Purcifull, D. 1972. CMI/AAB Descr. Pl. Viruses, 84:3
Rohlf, J. 2000. NTSYSpc: Numerical taxonomy and multivariate analysis system. Version 2.1. Users Guide, Exeter Software, Setauket, 38, New York
Smith, J.S.C., Chin, E.C.L., Shu, H., Smith, O.S., Wall, S.J., Senior, M.L., Mitchell, S.E., Kresovich, S. and Zeigle, J. 1997. An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree. Theor. Appl. Genet., 95:163-173
Sneath, P.H.A. and Sokal, R.R. 1973. Numerical Taxonomy: The Principles and Practice of Numerical Classification. W.F. Freeman and Co., San Francisco, California, USA, p. 573
Tanksley, S.D., Young, N.D., Paterson, A.H. and Bonierbale, M.W. 1989. RFLP mapping in plant breeding: New tools for an old science. Biotechnol., 7:257-264
Downloads
Published
Issue
Section
License
Copyright (c) 2014 R Sudha, T N Balamohan, K Soorianathasundaram, N Manivannan (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
