Evaluation of cucumber (Cucumis sativus L.) genotypes for drought toleranceusing high throughput PEG induced drought indices
DOI:
https://doi.org/10.24154/jhs.v20i2.4289Keywords:
Drought tolerance indices, germination traits, seedling stage drought stressAbstract
Cucumber is highly sensitive to drought due to its high-water requirement. Identifying genotypes that can tolerate early-stage moisture stress can reduce mortality and improve survival during drought. In this study, 16 genotypes were evaluated for seedling stage drought stress (SDS) tolerance using PEG8000 (18%). Five traits namely germination percentage, germination index, germination energy, shoot length (85.26% reduction) and root length were recorded and analyzed using five selection indices, namely arithmetic mean productivity (AMP), geometric mean productivity (GMP), harmonic mean productivity (HMP), stress tolerance index (STI) and membership function value (MFV). The indices were applied to assess the variability among genotypes and identify those tolerant to drought stress. Among the indices MFV and AMP were determined to be most desirable due to the better discriminating ability and strong correlation with mean shoot length. Based on the indices, the genotypes G9, G2 and W4 were identified as the most tolerant to PEG induced drought stress at seedling stage. Thus, the identified genotypes can be used in further breeding program of cucumber improvement.
Downloads
References
Agili, S., Nyende, B., Ngamau, K., & Masinde, P. (2012). Selection, yield evaluation, and drought tolerance indices of orange-fleshed sweet potato (Ipomoea batatas Lam.) hybrid clones. Journal of Nutrition & Food Sciences, 2(3), 138. https://doi.org/10.4172/2155-9600.1000138
Bennani, S., Nsarellah, N., Jlibene, M., Tadesse, W., Birouk, A., & Ouabbou, H. (2017). Efficiency of drought tolerance indices under different stress severities for bread wheat selection. Australian Journal of Crop Science, 11(4). https://doi.org/10.21475/ajcs.17.11.04.pne272
Bewley, J. D., & Black, M. (2013). Seeds: Physiology of development and germination. Springer Science & Business Media.
Bharathy, S., Ramesh, S., & Kalpana, M. (2024). Selection of end-of-season drought stress tolerant greengram (Vigna radiata (L.) Wilczek) genotypes of different maturity groups. Mysore Journal of Agricultural Sciences, 58(3).
Blum, A. (2011). Drought resistance—is it really a complex trait? Functional Plant Biology, 38(10), 753–757. https://doi.org/10.1071/FP11101
Das, A., Munshi, A. D., Raju, D., Kumar, S., Singh, D., Talukdar, A., & Dey, S. S. (2024). Key physiological traits for drought tolerance identified through phenotyping a large set of slicing cucumber (Cucumis sativus L.) genotypes under field and water-stress conditions. Genetic Resources and Crop Evolution, 71(5), 1855–1868. https://doi.org/10.1007/s10722-023-01737-y
Das, A., Singh, S., Islam, Z., Munshi, A. D., Behera, T. K., Dutta, S., & Dey, S. S. (2022). Current progress in genetic and genomics-aided breeding for stress resistance in cucumber (Cucumis sativus L.). Scientia Horticulturae, 300, 111059. https://doi.org/10.1016/j.scienta.2022.111059
Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms, and management. In Sustainable agriculture (pp. 153–188). Springer. https://doi.org/10.1007/978-90-481-2666-8_12
Farshadfar, E., & Elyasi, P. (2012). Screening quantitative indicators of drought tolerance in bread wheat (Triticum aestivum L.) landraces. European Journal of Experimental Biology, 2(3), 577–584.
Fernandez, G. C. J. (1992). Effective selection criteria for assessing plant stress tolerance. In C. G. Kuo (Ed.), Adaptation of food crops to temperature and water stress: Proceedings of an International Symposium (pp. 257–270). Taiwan.
Fischer, R. A., & Maurer, R. (1978). Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research, 29(5), 897–912. https://doi.org/10.1071/AR9780897
Hou, M., Yao, N., Li, Y., Liu, F., Biswas, A., Pulatov, A., & Hassan, I. (2022). Better drought index between SPEI and SMDI and the key parameters in denoting drought impacts on spring wheat yields in Qinghai, China. Agronomy, 12(7), 1552. https://doi.org/10.3390/agronomy12071552
Kalpana, M., Madhusudan, K., Ramesh, S., Siddu, C., Basanagouda, G., Priyadarshini, S., & Kanavi, M. (2023). Identification of terminal moisture stress tolerant dolichos bean (Lablab purpureus L. Sweet) recombinant inbred lines (RILs). Mysore Journal of Agricultural Sciences, 57(4).
Khakwani, A. A., Dennett, M. D., & Munir, M. (2011). Drought tolerance screening of wheat varieties by inducing water stress conditions. Songklanakarin Journal of Science & Technology, 33(2).
Khan, M. Q., Anwar, S., & Khan, M. I. (2002). Genetic variability for seedling traits in wheat (Triticum aestivum L.) under moisture stress conditions. Asian Journal of Plant Sciences, 1(5), 588–590. https://doi.org/10.3923/ajps.2002.588.590
Kłosińska, U., Kozik, E. U., Treder, W., & Klamkowski, K. (2016). Optimization of testing methods of cucumber for drought tolerance. In E. U. Kozik & H. S. Paris (Eds.), Proceedings of Cucurbitaceae 2016, the XIth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 146–150).
Li, D. M., Zhang, J., Sun, W. J., Li, Q., Dai, A. H., & Bai, J. G. (2011). 5-Aminolevulinic acid pretreatment mitigates drought stress of cucumber leaves through altering antioxidant enzyme activity. Scientia Horticulturae, 130(4), 820–828. https://doi.org/10.1016/j.scienta.2011.09.010
Li, W., Zhang, H., Zeng, Y., Xiang, L., Lei, Z., Huang, Q., & Cheng, Q. (2020). A salt tolerance evaluation method for sunflower (Helianthus annuus L.) at the seed germination stage. Scientific Reports, 10(1), 10626. https://doi.org/10.1038/s41598-020-67210-3
Liu, Z. J., Zhang, X. L., Bai, J. G., Suo, B. X., Xu, P. L., & Wang, L. (2009). Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. Scientia Horticulturae, 121(2), 138–143. https://doi.org/10.1016/j.scienta.2009.01.032
Mousavi, S. S., Yazdi Samadi, B., Naghavi, M. R., Zali, A. A., Dashti, H., & Pourshahbazi, A. (2008). Introduction of new indices to identify relative drought tolerance and resistance in wheat genotypes. Desert, 12, 165–178.
Rosielle, A. A., & Hamblin, J. (1981). Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science, 21(6), 943–946. https://doi.org/10.2135/cropsci1981.0011183X002100060033x
Verslues, P. E., Agarwal, M., Katiyar-Agarwal, S., Zhu, J., & Zhu, J. K. (2006). Methods and concepts in quantifying resistance to drought, salt, and freezing, abiotic stresses that affect plant water status. The Plant Journal, 45(4), 523–539. https://doi.org/10.1111/j.1365-313X.2005.02593.x
Xu, C., Mao, J., Li, X., Burner, D. M., Hussin, S. H., & Liu, X. (2023). Evaluation of drought tolerance in sugarcane genotypes using the membership function value of drought tolerance. Euphytica, 219(3), 37. https://doi.org/10.1007/s10681-023-03167-4
Yan, C., Song, S., Wang, W., Wang, C., Li, H., Wang, F., & Sun, X. (2020). Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought-tolerant coefficient of yield. BMC Plant Biology, 20, 1–15. https://doi.org/10.1186/s12870-020-02519-9
Yan, W. (1999). A study on the methodology of cultivar evaluation based on yield trial data with special reference to winter wheat in Ontario (Doctoral dissertation, University of Guelph).
Zadeh, L. A. (1965). Fuzzy sets. Information and Control, 8(3), 338–353. https://doi.org/10.1016/S0019-9958(65)90241-X
Downloads
Published
Data Availability Statement
None
Issue
Section
License
Copyright (c) 2025 K N Poornima, V Hegde, K S Shivashankara, K V Ravishankar, M Pitchaimuthu, S Shyamalamma (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.



. 








