Evaluation of cucumber (Cucumis sativus L.) genotypes for drought toleranceusing high throughput PEG induced drought indices

Authors

  • K N Poornima ICAR-IIHR , Indian Institute of Horticultural Research image/svg+xml Author
  • V Hegde ICAR-IIHR , Indian Institute of Horticultural Research image/svg+xml Author
  • K S Shivashankara ICAR-IIHR , Indian Institute of Horticultural Research image/svg+xml Author
  • K V Ravishankar ICAR-IIHR , Indian Institute of Horticultural Research image/svg+xml Author
  • M Pitchaimuthu ICAR-IIHR Author
  • S Shyamalamma University of Agricultural Sciences, Bangalore image/svg+xml , University of Agricultural Sciences, Bangalore Author

DOI:

https://doi.org/10.24154/jhs.v20i2.4289

Keywords:

Drought tolerance indices, germination traits, seedling stage drought stress

Abstract

Cucumber is highly sensitive to drought due to its high-water requirement. Identifying genotypes that can tolerate early-stage moisture stress can reduce mortality and improve survival during drought. In this study, 16 genotypes were evaluated for seedling stage drought stress (SDS) tolerance using PEG8000  (18%). Five traits namely germination percentage, germination index, germination energy, shoot length (85.26% reduction) and root length were recorded and analyzed using five selection indices, namely arithmetic mean productivity (AMP), geometric mean productivity (GMP), harmonic mean productivity (HMP), stress tolerance index (STI) and membership function value (MFV). The indices were applied to assess the variability among genotypes and identify those tolerant to drought stress. Among the indices MFV and AMP were determined to be most desirable due to the better discriminating ability and strong correlation with mean shoot length. Based on the indices, the genotypes G9, G2 and W4 were identified as the most tolerant to PEG induced drought stress at seedling stage. Thus, the identified genotypes can be used in further breeding program of cucumber improvement.

Downloads

Download data is not yet available.

Author Biographies

  • K N Poornima, ICAR-IIHR, Indian Institute of Horticultural Research

    Scientist, Division of Basic Sciences

  • V Hegde, ICAR-IIHR, Indian Institute of Horticultural Research

    Scientist, Division of vegetable sciences

  • K S Shivashankara, ICAR-IIHR, Indian Institute of Horticultural Research

    Principal Scientist, Division of Basic Sciences

  • K V Ravishankar, ICAR-IIHR, Indian Institute of Horticultural Research

    Principal Scientist, Division of Basic Sciences

  • M Pitchaimuthu, ICAR-IIHR

    Principal Scientist, Division of vegetable science

  • S Shyamalamma, University of Agricultural Sciences, Bangalore, University of Agricultural Sciences, Bangalore

    Professor and Head, Department of Plant Biotechnology

References

Agili, S., Nyende, B., Ngamau, K., & Masinde, P. (2012). Selection, yield evaluation, and drought tolerance indices of orange-fleshed sweet potato (Ipomoea batatas Lam.) hybrid clones. Journal of Nutrition & Food Sciences, 2(3), 138. https://doi.org/10.4172/2155-9600.1000138

Bennani, S., Nsarellah, N., Jlibene, M., Tadesse, W., Birouk, A., & Ouabbou, H. (2017). Efficiency of drought tolerance indices under different stress severities for bread wheat selection. Australian Journal of Crop Science, 11(4). https://doi.org/10.21475/ajcs.17.11.04.pne272

Bewley, J. D., & Black, M. (2013). Seeds: Physiology of development and germination. Springer Science & Business Media.

Bharathy, S., Ramesh, S., & Kalpana, M. (2024). Selection of end-of-season drought stress tolerant greengram (Vigna radiata (L.) Wilczek) genotypes of different maturity groups. Mysore Journal of Agricultural Sciences, 58(3).

Blum, A. (2011). Drought resistance—is it really a complex trait? Functional Plant Biology, 38(10), 753–757. https://doi.org/10.1071/FP11101

Das, A., Munshi, A. D., Raju, D., Kumar, S., Singh, D., Talukdar, A., & Dey, S. S. (2024). Key physiological traits for drought tolerance identified through phenotyping a large set of slicing cucumber (Cucumis sativus L.) genotypes under field and water-stress conditions. Genetic Resources and Crop Evolution, 71(5), 1855–1868. https://doi.org/10.1007/s10722-023-01737-y

Das, A., Singh, S., Islam, Z., Munshi, A. D., Behera, T. K., Dutta, S., & Dey, S. S. (2022). Current progress in genetic and genomics-aided breeding for stress resistance in cucumber (Cucumis sativus L.). Scientia Horticulturae, 300, 111059. https://doi.org/10.1016/j.scienta.2022.111059

Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms, and management. In Sustainable agriculture (pp. 153–188). Springer. https://doi.org/10.1007/978-90-481-2666-8_12

Farshadfar, E., & Elyasi, P. (2012). Screening quantitative indicators of drought tolerance in bread wheat (Triticum aestivum L.) landraces. European Journal of Experimental Biology, 2(3), 577–584.

Fernandez, G. C. J. (1992). Effective selection criteria for assessing plant stress tolerance. In C. G. Kuo (Ed.), Adaptation of food crops to temperature and water stress: Proceedings of an International Symposium (pp. 257–270). Taiwan.

Fischer, R. A., & Maurer, R. (1978). Drought resistance in spring wheat cultivars. I. Grain yield responses. Australian Journal of Agricultural Research, 29(5), 897–912. https://doi.org/10.1071/AR9780897

Hou, M., Yao, N., Li, Y., Liu, F., Biswas, A., Pulatov, A., & Hassan, I. (2022). Better drought index between SPEI and SMDI and the key parameters in denoting drought impacts on spring wheat yields in Qinghai, China. Agronomy, 12(7), 1552. https://doi.org/10.3390/agronomy12071552

Kalpana, M., Madhusudan, K., Ramesh, S., Siddu, C., Basanagouda, G., Priyadarshini, S., & Kanavi, M. (2023). Identification of terminal moisture stress tolerant dolichos bean (Lablab purpureus L. Sweet) recombinant inbred lines (RILs). Mysore Journal of Agricultural Sciences, 57(4).

Khakwani, A. A., Dennett, M. D., & Munir, M. (2011). Drought tolerance screening of wheat varieties by inducing water stress conditions. Songklanakarin Journal of Science & Technology, 33(2).

Khan, M. Q., Anwar, S., & Khan, M. I. (2002). Genetic variability for seedling traits in wheat (Triticum aestivum L.) under moisture stress conditions. Asian Journal of Plant Sciences, 1(5), 588–590. https://doi.org/10.3923/ajps.2002.588.590

Kłosińska, U., Kozik, E. U., Treder, W., & Klamkowski, K. (2016). Optimization of testing methods of cucumber for drought tolerance. In E. U. Kozik & H. S. Paris (Eds.), Proceedings of Cucurbitaceae 2016, the XIth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 146–150).

Li, D. M., Zhang, J., Sun, W. J., Li, Q., Dai, A. H., & Bai, J. G. (2011). 5-Aminolevulinic acid pretreatment mitigates drought stress of cucumber leaves through altering antioxidant enzyme activity. Scientia Horticulturae, 130(4), 820–828. https://doi.org/10.1016/j.scienta.2011.09.010

Li, W., Zhang, H., Zeng, Y., Xiang, L., Lei, Z., Huang, Q., & Cheng, Q. (2020). A salt tolerance evaluation method for sunflower (Helianthus annuus L.) at the seed germination stage. Scientific Reports, 10(1), 10626. https://doi.org/10.1038/s41598-020-67210-3

Liu, Z. J., Zhang, X. L., Bai, J. G., Suo, B. X., Xu, P. L., & Wang, L. (2009). Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. Scientia Horticulturae, 121(2), 138–143. https://doi.org/10.1016/j.scienta.2009.01.032

Mousavi, S. S., Yazdi Samadi, B., Naghavi, M. R., Zali, A. A., Dashti, H., & Pourshahbazi, A. (2008). Introduction of new indices to identify relative drought tolerance and resistance in wheat genotypes. Desert, 12, 165–178.

Rosielle, A. A., & Hamblin, J. (1981). Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science, 21(6), 943–946. https://doi.org/10.2135/cropsci1981.0011183X002100060033x

Verslues, P. E., Agarwal, M., Katiyar-Agarwal, S., Zhu, J., & Zhu, J. K. (2006). Methods and concepts in quantifying resistance to drought, salt, and freezing, abiotic stresses that affect plant water status. The Plant Journal, 45(4), 523–539. https://doi.org/10.1111/j.1365-313X.2005.02593.x

Xu, C., Mao, J., Li, X., Burner, D. M., Hussin, S. H., & Liu, X. (2023). Evaluation of drought tolerance in sugarcane genotypes using the membership function value of drought tolerance. Euphytica, 219(3), 37. https://doi.org/10.1007/s10681-023-03167-4

Yan, C., Song, S., Wang, W., Wang, C., Li, H., Wang, F., & Sun, X. (2020). Screening diverse soybean genotypes for drought tolerance by membership function value based on multiple traits and drought-tolerant coefficient of yield. BMC Plant Biology, 20, 1–15. https://doi.org/10.1186/s12870-020-02519-9

Yan, W. (1999). A study on the methodology of cultivar evaluation based on yield trial data with special reference to winter wheat in Ontario (Doctoral dissertation, University of Guelph).

Zadeh, L. A. (1965). Fuzzy sets. Information and Control, 8(3), 338–353. https://doi.org/10.1016/S0019-9958(65)90241-X

Downloads

Published

31-12-2025

Data Availability Statement

None

Issue

Section

Research Papers

How to Cite

Poornima, K. N., Hegde, V., Shivashankara, K. S., Ravishankar, K. V., Pitchaimuthu, M., & Shyamalamma, S. (2025). Evaluation of cucumber (Cucumis sativus L.) genotypes for drought toleranceusing high throughput PEG induced drought indices. Journal of Horticultural Sciences, 20(2). https://doi.org/10.24154/jhs.v20i2.4289

Similar Articles

1-10 of 297

You may also start an advanced similarity search for this article.

Most read articles by the same author(s)

1 2 3 4 5 > >>