Optimization of methodology for the extraction of polyphenolic compounds with antioxidant potential and α-glucosidase inhibitory activity from Jamun (Syzygium cumini L.) seeds
DOI:
https://doi.org/10.24154/jhs.v16i1.1075Keywords:
á-glucosidase inhibitory activity, Antioxidant potential, Flavonoids, Jamun seed powder, Natural antioxidants, PolyphenolsAbstract
Jamun (Syzygium cumini L.) seed is one of the rich sources of polyphenolic compounds with antioxidant potential and α-glucosidase inhibitory activity. A study was conducted to optimize the methodology for the extraction of polyphenolic compounds (total phenolic and flavonoid contents) with antioxidant potential and α-glucosidase inhibitory activity from Jamun seed powder. The study showed that the nature of solvent and extraction conditions had a significant effect on total phenolic content (TPC), total flavonoid content (TFC), antioxidant potential, and α-glucosidase inhibitory activity. The TPC varied between 6.0 (mg/g Jamun seed powder) for the acetone extract to 119.2 (mg/g) for 80% aqueous acetone extract and TFC varied between 1.06 mg/g for the acetone to 10.81 mg/g for the 80% aqueous methanol. From the study, it was apparent that an aqueous form of acetone (acetone: water 80:20, v/v) is a better solvent system for extraction of polyphenolic compounds with high antioxidant potential and α-glucosidase inhibitory activity. Ultrasonication for 60 min increased the efficiency of phenolic extraction.
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Apak, R., Guclu, K., Ozyurek, M. and Karademir, S.E. 2004. Novel total antioxidant capacity
index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability
in the presence of neocuproine: CUPRAC method. J. Agric. Food Chem., 52: 7970"7981.
Aqil, F., Gupta, A., Munagala, R., Jeyabalan, J., Kausar, H., Sharma, R. J., Singh, I. P. and
Gupta, R.C. 2012. Antioxidant and antiproliferative activities of anthocyanin/ ellagitannin-enriched extracts from Syzygium cumini L. (Jamun, the Indian Blackberry). Nutr. Cancer., 64(3): 428–438.
Arivalagan, M., Roy, T. K., Yasmeen, A. M., Pavithra, K. C., Jwala, P. N., Shivasankara, K. S.,
Manikantan, M. R., Hebbar, K. B. and Kanade, S. R. 2018. Extraction of phenolic compounds
with antioxidant potential from coconut (Cocos nucifera L.) testa and identification of phenolic
acids and flavonoids using UPLC coupled with TQD-MS/MS. LWT - Food Sci Technol., 92:
–126.
Arnao, M.B., Cano, A. and Acosta, M. 2001. The hydrophilic and lipophilic contribution to total
antioxidant activity. Food Chem., 73: 239–244.
Ayya, N., Nalwade, V and Khan, T.N. 2015. Effect of jamun (Syzygium cumini L.) seed powder
supplementation on blood glucose level of type-II diabetic subject. Food Sci. Res. J., 6(2):
-356.
Ayyanar, M. and Subash-babu, P. 2012. Syzygium cumini (L.) Skeels: a review of its
phytochemical constituents and traditional uses. Asian Pac. J. Trop. Biomed., 2:240–246
Benherlal, P. and Arumughan, C. 2007. Chemical composition and in vitro antioxidant studies on Syzygium cumini fruit. J. Sci. Food Agric., 87:2560–2569
Benzie, I.E.F. and Strain J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure
of ‘antioxidant power’: The FRAP assay. Anal. Biochem., 239: 70-76.
Brand-Williams, W., Cuvelier, M.E., and Berset, C. 1995. Use of free radical method to evaluate
antioxidant activity. LWT - Food Sci Technol., 28: 25–30.
Cano, A., Hernandez-Ruiz, J., Garcia-Canovas, F., Acosta, M. and Arnao, M.B. 1998. An endpoint method for estimation of the total antioxidant activity in plant material. Phytochem. Anal., 9: 196-202.
De Bona, K.S., Bonfanti, G., Bitencourt, P.E., da Silva, T.P., Borges, R.M., Boligon, A., Pigatto, A., Athayde, M.L., Moretto, M.B. 2016. Protective effect of gallic acid and Syzygium cumini
extract against oxidative stress-induced cellular injury in human lymphocytes. Drug Chem.
Toxicol., 39(3):256–263
Hameed F., Gupta N., Rahman R., Anjum N. and Nayik G.A. 2020. Jamun. In: Nayik G.A., Gull
A. (eds) Antioxidants in Fruits: Properties and Health Benefits. Springer, Singapore. https://
doi.org/10.1007/978-981-15-7285-2_32
Hayouni, A., Abedrabba, M., Bouix, M. and Hamdi, M. 2007. The effects of solvents and extraction method on the phenolic contents and biological activities in vitro of Tunisian Quercus
coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chem., 105: 1126–1134.
Hossain, H., Rahman, S.E., Akbar, P.N., Khan, T.A., Rahman, M., Jahan, I.R. 2016. HPLC profiling, antioxidant and in vivo anti-inflammatory activity of the ethanol extract of Syzygium jambos available in Bangladesh. BMC Res. Notes, 9:191–198.
Kim, Y., Keogh, J. B., and Clifton, P.M. 2016. Polyphenols and glycemic control. Nutrients, 8, E17.
Khoddami, A., Meredith A.W. and Thomas H.R. 2013. Techniques for Analysis of Plant Phenolic
Compounds. Molecules, 18, 2328-2375
Mahmood, N. 2016. A review of α-amylase inhibitors on weight loss and glycemic control in
pathological state such as obesity and diabetes. Comp. Clin. Pathol., 25: 1253–1264. https://
doi.org/10.1007/s00580-014-1967-x
Omar, R., Li, L., Yuan, T., and Seeram NP. 2012. α Glucosidase Inhibitory hydrolyzable tannins
from Eugenia jambolana seeds. J. Nat. Prod., 75, 1505"1509
SAS. 2011. Statistical Analysis Software System, Version 9.3. SAS Institute, Cary, NC, USA.
Sim, L. Quezada-Calvillo, R. Sterchi, E.E. Nichols, B.L. Rose D.R. 2008. Human intestinal
maltase–glucoamylase: crystal structure of the N-terminal catalytic subunit and basis of
inhibition and substrate specificity J. Mol. Biol., 375(3): 782-792.
Singleton, V.L. Orthofer, R., and Lamuela-Raventos R.M. 1999. Analysis of total phenols and
otheroOxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods
Enzymol., 299:265–275.
Sulaiman, S.F., Sajak, A.A.B., Supriatno, K.L.O. and Seow, E.M. 2011. Effect of solvents in
extracting polyphenols and antioxidants of selected raw vegetables. J. Food Compos.
Anal., 24: 506–515.
Sun, T. and Ho, H. 2005. Antioxidant activities of buckwheat extracts. Food Chem., 90: 743–749.
Turkmen, N., Sari, F. and Velioglu, Y.S. 2006. Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenols determined by ferrous tartrate and Folin-Ciocalteu methods. Food Chem., 99: 835–841.
Wijekoon, M.M.J.O., Bhat, R. and Karim, A.A. 2011. Effect of extraction solvents on the phenolic compounds and antioxidant activities of bungakantan (Etlingera elatior Jack.) inflorescence. J. Food Compos. Anal., 24: 615–619
Zhao, H., Dong, J., Lu, J., Chen, J., Li, Y., Shan, L., Lin, Y., Fan, W. and Gu, G. 2006. Effect
of extraction solvent mixtures on antioxidant activity evaluation and their extraction capacity and selectivity for free phenolic compounds in barley (Hordeum vulgare L.). J. Agric. Food Chem., 54: 7277–7286.
Zhishen, J., Mengcheng, T. and Jianming, W. 1999. The determination of flavonoid contents in
mulberry and their scavenging effects on superoxide radicals. Food chem., 64: 555-559.
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