Genetic inheritance and identification of molecular markers linked tomale sterility in African marigold (Tagetes erecta L.)

Authors

  • Sumalatha Akkalareddy Indian Agriculture Research Institute, Assam , Indian Agriculture Research Institute, Assam Author
  • D C Lakshmana Reddy ICAR -Indian Institute of Horticultural Research, Bengaluru , Indian Institute of Horticultural Research image/svg+xml Author
  • M Arivalagan ICAR -Indian Institute of Horticultural Research, Bengaluru , Indian Institute of Horticultural Research image/svg+xml Author
  • V V Bhaskar Dr. Y.S.R. Horticultural University , Dr. Y.S.R Horticultural University image/svg+xml Author
  • Tejaswini Prakash ICAR-Indian Institute of Horticultural Research, Bengaluru , Indian Institute of Horticultural Research image/svg+xml Author

DOI:

https://doi.org/10.24154/jhs.v20i1.2290

Keywords:

African marigold, Mendelian genetics, male sterility, petaloid flower, SSR markers

Abstract

The demand for hybrid varieties of African marigold (Tagetes erecta L.) is on the rise due to the increased productivity and uniformity exhibited by F1 hybrids. To develop hybrids in marigold, male sterile line is essential, as emasculation is complex due to the unique flower structure of marigold. Inheritance of petaloid male sterility was investigated in a seed propagated line across six generations (P1, P2, F1, F2, BC1 and BC2), that confirmed a single dominant gene governing petaloid male sterility over multiple seasons. The marker CPSSR-39 exhibited clear and consistent segregation, following a Mendelian ratio 1:1 in F1 which is in accordance with the genes governing petaloid male sterility. This is the first report of genic petaloid male sterility with a linked marker marking a significant advancement in heterosis breeding of marigold.

Downloads

Download data is not yet available.

Author Biographies

  • Sumalatha Akkalareddy, Indian Agriculture Research Institute, Assam, Indian Agriculture Research Institute, Assam

    Scientist, ICAR-Indian Agriculture Research Institute, Gogamukh 787035, Assam, India

  • D C Lakshmana Reddy, ICAR -Indian Institute of Horticultural Research, Bengaluru, Indian Institute of Horticultural Research

    ICAR -Indian Institute of Horticultural Research, Bengaluru 560089, India

  • M Arivalagan, ICAR -Indian Institute of Horticultural Research, Bengaluru, Indian Institute of Horticultural Research

    ICAR -Indian Institute of Horticultural Research, Bengaluru 560089, India

  • V V Bhaskar, Dr. Y.S.R. Horticultural University, Dr. Y.S.R Horticultural University

    Dr. Y.S.R. Horticultural University, West Godavari, 534101, Andhra Pradesh, India

  • Tejaswini Prakash, ICAR-Indian Institute of Horticultural Research, Bengaluru, Indian Institute of Horticultural Research

    ICAR -Indian Institute of Horticultural Research, Bengaluru 560089, India

References

Ai, Y., Zhang, Q., Wang, W., Zhang, C., Cao, Z., Bao, M., & He, Y. (2016). Transcriptomic analysis of differentially expressed genes during flower organ development in genetic male sterile and male fertile *Tagetes erecta* by digital gene-expression profiling. *PLoS ONE, 11*(3), e0150892.

https://doi.org/10.1371/journal.pone.0150892

Asha, K. M. (2019a). *In silico mining of SSRs and mapping for genetic male sterility in marigold (Tagetes erecta L.)* [Doctoral dissertation, UAS, Bhagalkot].

Asha, K. M., Anuradha, S., Tejaswini, D. C., Lakshaman Reddy, S., Sateesha, R. P., Sarvamangala, S. C., Mahantesha, B. N., Naika, & Raghavendra, G. (2019b). Validation of SCAR marker linked to genic male sterility in marigold: As a forward step towards marker assisted breeding programme. *International Journal of Current Microbiology and Applied Sciences, 8*(2), 3373-3383.

https://doi.org/10.20546/ijcmas.2019.802.393

Berman, J., Zorrilla-López, U., Farré, G., Zhu, C., Sandmann, G., Twyman, R. M., & Christou, P. (2015). Nutritionally important carotenoids as consumer products. *Phytochemistry Reviews, 14*(5), 727-743.

https://doi.org/10.1007/s11101-014-9373-1

Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. *Focus, 12*, 13-15.

Du, M., Zhou, K., Liu, Y., Deng, L., Zhang, X., Lin, L., & Li, C. (2020). A biotechnology based male sterility system for hybrid seed production in tomato. *The Plant Journal, 102*(5), 1090-1100.

https://doi.org/10.1111/tpj.14678

Gupta, Y. C., Raghava, S. P. S., & Misra, R. L. (1999). Inheritance of male-sterile apetalous inflorescence in African marigold. *Journal of Ornamental Horticulture, 2*(2), 65-66.

He, Y. H., Ning, G. G., Sun, Y. L., Hu, Y., Zhao, X. Y., & Bao, M. Z. (2010). Cytological and mapping analysis of a novel male sterile type resulting from spontaneous floral organ homeotic conversion in marigold (*Tagetes erecta* L.). *Molecular Breeding, 26*, 19-29.

https://doi.org/10.1007/s11032-009-9372-x

Joshi, A. K., & Nabi, A. (2018). Genetics of inheritance of growth, yield and male sterility in *Capsicum annuum* L. *Journal of Pharmacognosy and Phytochemistry, 7*(1), 1682-1688.

Kumar, K. R., Singh, K. P., Raju, D. V. S., Panwar, S., Bhatia, R., Jain, P. K., & Kumar, V. (2017). Standardization of rapid multiplication protocol in petaloid male sterile lines of African marigold (*Tagetes erecta*) through in vitro culture. *Indian Journal of Agricultural Sciences, 87*(10), 31-38.

https://doi.org/10.56093/ijas.v87i10.74827

Quinn, G. P., & Keough, M. J. (2002). *Experimental design and data analysis for biologists*. Cambridge University Press. [https://doi.org/10.1017/S0021859603213241](https://doi.org/10.1017/S0021859603213241)

https://doi.org/10.1017/S0021859603213241

Rodrigues, D. B., Mercadante, A. Z., & Mariutti, L. R. B. (2019). Marigold carotenoids: Much more than lutein esters. *Food Research International, 119*, 653-664. [https://doi.org/10.3390/foods12193549](https://doi.org/10.3390/foods12193549)

https://doi.org/10.3390/foods12193549

Santosh, N. (2018). *Genetic and biochemical analysis of yield and quality parameters in marigold* [Doctoral dissertation, UAS, Bhagalkot].

SAS Institute. (2012). *SAS 9.3 statistical analysis system* (Version 9.3). Cary, NC: SAS Institute.

Saxena, K. B., Sultana, R., Mallikarjuna, N., Saxena, R. K., Kumar, R. V., Sawargaonkar, S. L., & Varshney, R. K. (2010). Male sterility systems in pigeon pea and their role in enhancing yield. *Plant Breeding, 129*(2), 125-134. [https://doi.org/10.3389/fgene.2022.1048476](https://doi.org/10.3389/fgene.2022.1048476)

https://doi.org/10.3389/fgene.2022.1048476

Tejaswini, Anuradha, S., & Archana, G. (2016a). IIHRMGYP-1 (IC0613361; INGR15036), a marigold (*Tagetes erecta* L.) germplasm with petaloid sterility flowers; ability to be multiplied by cuttings. *Indian Journal of Plant Genetic Resources, 29*(2), 221-222.

Tejaswini, Anuradha, S., Archana, G., & Ghatke, M. (2016b). Characterisation and utilization of three distinct male sterile systems in marigold (*Tagetes erecta* L.). *Indian Journal of Agricultural Sciences, 86*(10), 1271-1275.

https://doi.org/10.56093/ijas.v86i10.62101

Downloads

Published

31-08-2025

Issue

Section

Research Papers

How to Cite

Akkalareddy, S., Lakshmana Reddy, D. C., Arivalagan, M., Bhaskar, V. V., & Prakash, T. (2025). Genetic inheritance and identification of molecular markers linked tomale sterility in African marigold (Tagetes erecta L.). Journal of Horticultural Sciences, 20(1). https://doi.org/10.24154/jhs.v20i1.2290

Most read articles by the same author(s)