Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.)

Authors

  • K V Ravishankar Author
  • T S Aghora Author
  • N Mohan Author
  • A H Naveen Author
  • M Krishnareddy Author

DOI:

https://doi.org/10.24154/jhs.v4i2.538

Keywords:

Bulk Segregant Analysis, MYMV, Phaseolus vulgaris L., RAPD Marker

Abstract

Mungbean yellow mosaic virus (MYMV) causes yield loss up to 80 % and is becoming problematic in French bean growing areas. Molecular marker linked selection to MYMV resistance is helpful in rapid identification of genotypes carrying resistant genes. Hence, the present study was undertaken to identify the RAPD marker associated with MYMV resistance in French bean (Phaseolus vulgaris L.). Bulk segregant analysis (BSA) was used to identify RAPD marker linked to MYMV resistance. More than 140 random decamers were surveyed for identification of polymorphic markers between the DNA bulks of resistant and susceptible F2 individuals and their contrasting parents. Ninety eight per cent of these primers amplified DNA in both parents and bulks. Twenty two primers produced specific bands for resistant parent which was absent in the susceptible parent. Out of 22 primers, four primers produced specific fragments viz., OPG 13458, OPX 5670, OPW 17380 and OPP 07730, respectively in resistant parent and bulk, which were absent in susceptible parent and bulk. Amplification of individual DNA samples of segregating F2 resistant individuals using putative marker, OPP 07730, a decamer revealed polymorphism in all four resistant and four susceptible F2 segregants, indicating that the marker OPP 07730 was associated with MYMV resistance in IC-525260, a resistant genotype.

References

Adam-Blondon, A.F., Sevignac, M., Bannerot, H. and Dron, M.1994. SCAR, RAPD and RFLP markers linked to dominant gene (Are) conferring resistance to anthracnose in common bean. Theor. and Appl. Genet., 88:863-870

Aghora, T.S., Mohan, N. and Krishana reddy, M. 2008. Identification of stable sources of resistance to Mung bean yellow mosaic virus in french bean (Phaseolus vulgaris L.). Ind. J. Virol., 19:281

Arumuganatham, K. and Earle, E. D.1991. Nuclear DNA content of some important plant species. Pl. Mol.Biol.Rep., 9:208-218

Beckmann, J. S. and Soller, M. 1986. Restriction fragment length polymorphisms and genetic improvement of agricultural species. Euphytica, 35:111-124

Beebe, S. E., Ochoa, I., Skroch, P., Nienhuis, J. and Tivang, J.1995. Genetic diversity among common bean breeding lines developed for Central America. Crop Sci., 35:1178-1183

Haley, S., Mikals, P. N., Stavely, J. R., Byrum, J. D. and Kuully, E.1993. Identification of RAPD markers linked to a major rust resistance gene block in common bean. Theor. and Appl. Genet., 86:505-512

Hu, X. Y., Ohm, H. W. and Dweikat, I.1997. Identification of RAPD markers linked to the gene pm 1 for resistance to powdery mildew in wheat. Theor. and Appl. Genet., 94:832-840

Jones, C.J., Edwards, K.J., Castaglione, S., Winfield, M.O., Sala, F. Van deWiel, C., Bredemeijer, G., Vosman, B., Matthes, M., Daly, A., Brettschneider, R., Bettini, P. Buiatti, M., Maestri, E., Malcevschi, A., Marmiroli, N., Aert, R., Volckaert, G., Rueda, J., Linacero, R., Vazquez, A. and Karp, A. 1997. Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories. Mol. Breed., 3:381-390

Morales, F. J. 2001. Conventional breeding for resistance to Bemisia tabaci transmitted geminiviruses. Crop Prot., 20:825-834

Nariani, T. K., 1960, Yellow mosaic of mung (Phaseolus aureus L.) Ind. Phytopath., 13:24-29

Nietsche, S., Borem, A., Carvalho, G. A., Rocha, R. C., Paulajr, T, J., Debarros E. G. and Moreisa, M. A.2000. RAPD and SCAR markers linked to a gene confirming resistance to angular leaf spot in common bean. J. Phytopath., 148:117-121

Ohmori, T., Murata, M. and Motoyoshi, F. 1995. Identification of RAPD markers linked to the Tm-2 locus in tomato. Theor. and Appl. Genet., 90:307-311

Poulsen, D. M. E., Henry, R. J., Jhonston, R. P., Irwin, J. A. G. and Ress, R. G. 2000. The use of bulk segregant analysis to identify a RAPD marker linked to leaf rust resistance in barley. Theor. Appl. Genet., 91:270-273

Ravishankar, K.V., Lalitha Anand and Dinesh, M.R. 2000. Assessment of genetic relatedness among a few Indian mango varieties using RAPD marker. J. Hort. Sci. Biotech. 75:198-201

Selvi, R., Muthiah, A. R., Manivannan, N., Raveendran, T. S., Manickam and Samiyappan, 2006, Tagging of RAPD marker for MYMV resistance in mung bean (Vigna radiate (L.) Wilczek). Asian J. Plant Sci, 5:277- 280

Singh, R. A., De. R. K., Gurha, S. N., Ghoush, A. and Vishwa, D. 2004. The Mung bean Yellow Mosaic Virus disease. (Technical Bulletin) Indian Institute of Pulse Research, Kanpur

Singh, S. P., Morales, F. J., Miklas, P. N. and Teran, H. 2000. Selection for bean golden mosaic resistance in intra and interracial bean populations. Crop Sci., 40:1565-1572

Stevens, K. R., Lamb, E. M. and Rhoads, D. D.1995. Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomato using RAPD and RFLP analysis. Theo. Appl. Genet., 90: 451-456

Tanksley, S. D.1983. Molecular markers in plant breeding. Plant. Mol. Biol. Rep., 1:3-8

Thongmeearkjom, P., Kittipakorn, K. and Surin, P. 1981. Outbreak of mungbean yellow mosaic disease in Thailand. Thai. J. Agric. Sci., 14:201-206

Urrea, A. C. and Mikals, N. P. 1996. A co-dominant randomly amplified polymorphic DNA (RAPD) marker useful for indirect selection of bean golden mosaic virus resistance in common bean. J. Amer. Sco. Hort. Sci., 121:1035-1039

Williams, J. G. K., Kubelik, A. R. and Livak, K. J., 1990. Oligonucleotide primers of arbitrary sequence amplified DNA polymorphisms which are useful as genetic markers. Nucleic Acids Res., 18:6531-6535

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Published

31-12-2009

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Short Communications

How to Cite

Ravishankar, K. V., Aghora, T. S., Mohan, N., Naveen, A. H., & Krishnareddy, M. (2009). Identification of RAPD Marker Linked to Mungbean Yellow Mosaic Virus Resistance in French Bean (Phaseolus vulgaris L.). Journal of Horticultural Sciences, 4(2), 167-169. https://doi.org/10.24154/jhs.v4i2.538

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