Multivariate Based Marker Analysis in Turmeric (Curcuma longa L.)

Authors

  • K R Vijayalatha Author
  • N Chezhiyan Author

DOI:

https://doi.org/10.24154/jhs.v3i2.568

Keywords:

Genetic Divergence, Turmeric, Marker, Polymorphism, Primer

Abstract

Genetic diversity of 30 accessions of turmeric was assessed at the molecular level and compared to morphological traits for degree of divergence. The pattern of clustering of quantitative data based on D2, K means and UPGMA revealed discrepancy among them. The cluster profile, based on quantitative data and RAPD markers exhibited considerable levels of congruence between them. Accessions studied for degree of divergence by RAPD profiles revealed 68.50% polymorphism for 21 primers. The highest number of fragments (10) was obtained with primer OPG 19 while OPC 18 was completely monomorphic. Primers OPB 08, OPC 20, OPE 09 and OPG 19 detected a high level of polymorphism (> 90%). Discrepancy observed at both morphological and molecular levels in the accessions emphasizes the need for specific morphological and molecular markers for discriminating these accessions.

References

Mahalonobis, P.C. 1936. On the generalise distance in statistics. Proc. Natl. Instt. Sci., 2:49-55

McCouch, S.R., Kochert, G. Yu, Z.H. Wang Z.Y., Khush, G.S.. Coffman W.R and Tanksley. S.D. 1988. Molecular mapping of rice chromosomes. Theor.Appl. Genet., 76:815-829

Sasikumar, B. 2005. Genetic resources of Curcuma: diversity, characterization and utilization. Plant Gen. Res. C&U 3: 230-251

Shamina, A., Zachariah, T.J., Sasikumar, B and George, J.K. 1998. Biochemical variation in turmeric (Curcuma longa L.) accessions based on isozyme polymorhism. J. Hortl. Sci. and Biotech., 73:479-483

Sneath, P.H.A. and Sokal, R.R. 1973. Principles of Numerical Taxonomy. San Francisco, W.H. Freeman

Syamkumar, S and Sasikumar. B. 2007. Molecular marker based genetic diversity analysis of Curcuma species from India. Sci. Horti., 112:235-241

Williams, J.G.K., Kubelik, A.R., Livek, K.J., Rafalski, J and Tingey. S.V. 1990. DNA polymorphisms amplified by arbitary primers are useful as genetic markers. Nucl. Acid Res., 18:6531-6535

Zachariah, T.J. and Babu. K.N. 1992. Effect of storage of fresh turmeric, oleoresin and curcumin content. J. Spices and Aromatic Crops, 1:55-58

Downloads

Published

31-12-2008

Issue

Section

Research Papers

How to Cite

Vijayalatha, K. R., & Chezhiyan, N. (2008). Multivariate Based Marker Analysis in Turmeric (Curcuma longa L.). Journal of Horticultural Sciences, 3(2), 107-111. https://doi.org/10.24154/jhs.v3i2.568

Similar Articles

1-10 of 121

You may also start an advanced similarity search for this article.