Genetic analysis and identification of SSR marker linked topowdery mildew resistance in bitter gourd (Momordica charantia L.)

Authors

  • K Prasanth Kerala Agricultural University, Thrissur - 680 656, Kerala, India Author https://orcid.org/0000-0002-2675-8237
  • D C Lakshmana Reddy ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author
  • S Sriram ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author
  • R Venugopalan ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author
  • M Pitchaimuthu ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author
  • K Hima Bindu ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author https://orcid.org/0000-0002-0556-8151
  • B Varalakshmi ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India Author

DOI:

https://doi.org/10.24154/jhs.v19i1.2264

Keywords:

Bitter gourd, inheritance, powdery mildew, resistance, SSR marker

Abstract

The inheritance of powdery mildew resistance in bitter gourd resistant line IIHR-144-1 was studied in cross with two susceptible lines Arka Harit and IIHR Sel-5-8. The segregation ratios in F2 and backcross progenies indicated that resistance is governed by dominant-recessive epistasis or dominant inhibitory epistasis, indicating the involvement of more than one pairs of genes, one dominant and one recessive for resistance along with other background minor genes in the resistant line IIHR-144-1. Bulk segregant analysis of F2 population of cross between IIHR-144-1 and Arka Harit using SSR markers identified McSSR 57 produced a polymorphic DNA fragment that co-segregated with disease reaction. Considering the cross compatibility between Momordica charantia L. and M. charantia var. muricata (IIHR-144-1) the resistance trait can be transferred to cultivated species through hybridization and selection.

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Author Biographies

  • K Prasanth , Kerala Agricultural University, Thrissur - 680 656, Kerala, India

    Department of Vegetable Science, Kerala Agricultural University, Thrissur - 680 656, Kerala, India

  • D C Lakshmana Reddy, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Basic Sciences, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

  • S Sriram , ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Crop Protection, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

  • R Venugopalan , ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Social Sciences & Training, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

  • M Pitchaimuthu , ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Vegetable Crops, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

  • K Hima Bindu, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Flower and Medicinal Crops, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

  • B Varalakshmi, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

    Division of Vegetable Crops, ICAR- Indian Institute of Horticultural Research, Bengaluru - 560 089, India

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Published

06-06-2024

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Section

Original Research Papers

How to Cite

Prasanth , K., Lakshmana Reddy, . D. C., Sriram , S., Venugopalan , R., Pitchaimuthu , M., Hima Bindu, K., & Varalakshmi, B. (2024). Genetic analysis and identification of SSR marker linked topowdery mildew resistance in bitter gourd (Momordica charantia L.). Journal of Horticultural Sciences, 19(1). https://doi.org/10.24154/jhs.v19i1.2264

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