Development and genetic evaluation of recombinant inbred line population for yield and its attributing traits and marker assisted selection for bacterial wilt resistance in tomato
DOI:
https://doi.org/10.24154/jhs.v19i2.1945Keywords:
DNA markers, GCV, heritablity, PCV, Ralstonia solanacearumAbstract
A total of 147 recombinant inbred lines were developed from the cross Anagha x FBT-41 using single seed descent method to evaluate genetic parameters and identification of superior inbred lines using marker assisted selection. The analysis of variance revealed that there is a significant variation was observed between the lines for all the traits studied. Skewness values ranged from 0.16 (number of cluster per plant and number of locules per fruit) to 1.16 (TSS) and kurtosis ranged from 1.73 (number of clusters plant and number of locules per fruit) to 4.1 (TSS) which follows normal distribution suggesting that the involvement of multiple genes with quantitative nature of inheritance. Higher PCV, GCV, h2bs and GAM observed in plant height , number of branches per plant , number of cluster per plant , number of fruits per cluster , fruit locules , fruit length , fruit diameter, average fruit weight , number of fruits per plant and yield per plant indicated additive nature of gene action so there is scope for selection. Out of four bacterial wilt linked marker used in this study only one SCAR marker i.e., SCU176-534 showed polymorphism between parents so it is used for marker assisted selection to identify superior inbred lines coupled with bacterial wilt resistance. A total seven superior inbred lines were selected coupled with bacterial wit resistance. This lines can be used in further breeding programme as directly released as variety or use as parents to develope bacterial wilt resistant hybrids.
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Agong, S. G., Schittenhelm, S., & Friedt, W. (2000). Genotypic variation of Kenyan tomato (Lycopersicon esculentum L.) germplasm. The Journal of Food Technology in Africa. https://doi.org/10.4314/jfta.v6i1.19277
Akhter, M., Apon, F. N., Bhuiyan, M. M. R., Siddique, A. B., Husna, A., & Zeba, N. (2021). Genetic variability, correlation coefficient, path coefficient and principal component analysis in tomato (Solanum lycopersicum L.) genotypes. Plant Cell Biotechnology and Molecular Biology, 22(33–34), 46–59. https://ikprress.org/index.php/PCBMB/article/view/6160
Aslam, M. N., Mukhtar, T., Hussain, M. A., & Raheel, M. (2017). Assessment of resistance to bacterial wilt incited by Ralstonia solanacearum in tomato germplasm. Journal of Plant Diseases and Protection, 124(6), 585–590. https://doi.org/10.1007/s41348-017-0100-1
Chaudhary, L. B., & Prasad, B. (1968). Genetic variation and heritability of quantitative characters in Indian mustard (Brassica juncea). Indian Journal of Agricultural Sciences, 38, 820–825.
Darshan, S. P., Reshma, J. K., & Mathew, A. (2013). Estimation of lycopene content in different tomato varieties and its commercial products. Australasian Journal of Environmental Management, 8(2), 122–124.
Federer, W. T., & Raghavrao, D. (1975). On augmented design. Biometrics, 31(1), 29–35.
Gawel, N. J., & Jarret, R. L. (1991). A modified CTAB DNA extraction procedure for Musa and Ipomoea. Plant Molecular Biology Reporter, 9(3), 262–266. https://doi.org/10.1007/BF02672076
Glaszmann, J. C., Kilian, B., Upadhyaya, H. D., & Varshney, R. K. (2010). Accessing genetic diversity for crop improvement. Current Opinion in Plant Biology, 13(2), 167–173. https://doi.org/10.1016/j.pbi.2010.01.004
Hanson, C. H., Robinson, H. R., & Comstock, R. S. (1956). Biometrical studies of yield in segregating population of Korean lespedeza. Agronomy Journal, 48, 268–272.
Jayaramachandran, M., Kumaravadivel, N., Eapen, S., & Kandasamy, G. (2010). Gene action for yield attributing characters in segregating generation (M2) of sorghum (Sorghum bicolor L.). Electronic Journal of Plant Breeding, 1(4), 802–805.
Jenkins, J. A. (1948). The origin of the cultivated tomato. Economic Botany, 2(4), 379–392. https://doi.org/10.1007/BF02859492
Johnson, H. W., Robinson, H. F., & Comstock, R. E. (1955). Estimation of genetic and environmental variability in soybean. Agronomy Journal, 47, 314–318. http://dx.doi.org/10.2134/agronj1955.00021962004700070009x
Lush, J. L. (1940). Intra-sire correlation and regression of offspring on dams as a method of estimating heritability of characters. In Proceedings of the American Society of Animal Production (Vol. 33, pp. 293–301).
Mohan, M., Nair, S., Bhagwat, A., Krishna, T. G., Yano, M., Bhatia, C. R., & Sasaki, T. (1997). Genome mapping, molecular markers and marker-assisted selection in crop plants. Molecular Breeding, 3(2), 87–103. https://doi.org/10.1023/A:1009651919792
Pandiyaraj, P., Singh, T. H., Reddy, K. M., Sadashiva, A. T., Gopalakrishnan, C., Reddy, A. C., Pattanaik, A., & Reddy, D. L. (2019). Molecular markers linked to bacterial wilt (Ralstonia solanacearum) resistance gene loci in eggplant (Solanum melongena L.). Crop Protection, 124, 104822. https://doi.org/10.1016/j.cropro.2019.05.016
Pearson, K. (1894). Contributions to the mathematical theory of evolution. Philosophical Transactions of the Royal Society of London. Series A, Containing Papers of a Mathematical or Physical Character, 185, 71–110. https://doi.org/10.1098/rsta.1894.0003
Rick, C. M. (1969). Controlled introgression of chromosomes of Solanum pennellii into Lycopersicon esculentum: Segregation and recombination. Genetics, 62(4), 753–768. https://doi.org/10.1093/genetics/62.4.753
Sadasivam, S., & Manickam, A. (1992). Biochemical methods for agricultural sciences. Wiley Eastern Limited.
Sujeet, K. S., Ramanjini Gowda, P. H., Saikia, B., Debbarma, J., Velmurugan, N., & Chikkaputtaiah, C. (2018). Screening of tomato genotypes against bacterial wilt (Ralstonia solanacearum) and validation of resistance-linked DNA markers. Australasian Plant Pathology, 47(6), 365–374. https://doi.org/10.1007/s13313-018-0567-7
Truong, H. T. H., Kim, S., Tran, H. N., Nguyen, T. T. T., Nguyen, L. T., & Hoang, T. K. (2015). Development of a SCAR marker linked to bacterial wilt (Ralstonia solanacearum) resistance in tomato line Hawaii 7996 using bulked-segregant analysis. Horticulture, Environment, and Biotechnology, 56(4), 506–515. https://doi.org/10.1007/s13580-015-1050-9
Winstead, N. N., & Kelman, A. (1952). Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology, 42(11), 628–634.
Yang, W., & Francis, D. M. (2007). Genetics and breeding for resistance to bacterial diseases in tomato: Prospects for marker-assisted selection. In Genetic Improvement of Solanaceae Crops (Vol. 2, pp. 379–419).
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