Molecular Exploration of Guava (Psidium guajava L.) Genome Using SSR and RAPD Markers: A Step towards Establishing Linkage Map
DOI:
https://doi.org/10.24154/jhs.v10i2.118Keywords:
Guava, Genotyping, M13-Tailed PCR, SSR, RAPDAbstract
In the present study, molecular evaluation of two guava mapping populations (MP), MPI comprising 94 F1 progenies and MPII comprising 46 F1 progenies, was carried out using simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) markers. A pseudo-test cross strategy was implemented where 'Kamsari' X 'Purple Local' and 'Purple Local' X 'Allahabad Safeda' were crossed, and, these showed variation in fruit quality traits such as seedstrength (hardness/softness), fruit weight, TSS and pulp color. A set of 30 RAPD markers was used for genotyping MPI while a set of 55 SSR markers was used for genotyping MPII. In case of MPI, 30 RAPD markers generated 214 scorable markers, of which 80 markers were specific to 'Kamsari', 14 markers to 'Purple Local' and the remaining 120 were intercross markers. As for MPII, 55 polymorphic SSR markers resulted in generation of 207 alleles (with a maximum of 4 alleles and a minimum of 3 alleles per locus), of which 108 alleles were specific to 'Purple Local' while 99 were specific to 'Allahabad Safeda'. Genotypic data thus generated can be further exploited for constructing genetic linkage maps and mapping complex QTLs governing fruit quality traits in guava..
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Aranguren, Y., Valecillos, C. and Fermin, G. 2010. Assessment of the variability of Venezuelan guava landraces by microsatellites. Acta Hort., 849:147-154 DOI: https://doi.org/10.17660/ActaHortic.2010.849.16
Briceno, A., Aranguren, Y. and Fermin, G. 2010. Assessment of guava-derived SSR markers for the molecular characterization of Myrtaceae from different ecosystems in Venezuela. IInd International Symposium on Guava and other Myrtaceae. Acta Hort., 849:139-146 DOI: https://doi.org/10.17660/ActaHortic.2010.849.15
Chandra, R. and Mishra, M. 2007. Biotechnological interventions for improvement of guava (Psidium guajava L.). Acta Hort., 735:117-125 DOI: https://doi.org/10.17660/ActaHortic.2007.735.15
Coser, M.S., Fontes, M.P.M. and Ferreira, F.S.M. 2012. Pollen viability of guava genotypes from different locations. In: 3rd International Symposium on Guava and Other Myrtaceae, Petrolina, PE, Brazil. Session III: Myrtaceae Plant Breeding and Post-Harvest Management
Das, M., Banerjee, S., Dhariwal, R., Vyas, S., Mir, R.R., Topdar, N., Kundu, A., Khurana, J.P., Tyagi, A.K., Sarkar, D., Sinha, M.K., Balyan, H.S. and Gupta, P.K. 2012. Development of SSR markers and construction of a linkage map in jute. J. Genet., 91:21-31 DOI: https://doi.org/10.1007/s12041-012-0151-9
Dinesh, M.R. and Vasugi, C. 2010. Phenotypic and genotypic variations in fruit characteristics of guava (Psidium guajava). Indian J. Agri’l. Sci., 80:998-999
Grando, M.S., Bellin, D., Edwards, K.J., Pozzi, C., Stefanini, M. and Velasco, R. 2003. Molecular linkage maps of Vitis vinifera L. and Vitis riparia Mchx. Theor. Appl. Genet., 106:1213-1224 DOI: https://doi.org/10.1007/s00122-002-1170-3
Grattapaglia, D. and Sederoff, R. 1994. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-test cross: mapping strategy and RAPD markers. Genetics, 137:1121-1137 DOI: https://doi.org/10.1093/genetics/137.4.1121
Gutierrez, R.M.P., Mitchell, S. and Solis, R.V. 2008. Psidium guajava: A review of its traditional uses, phytochemistry and pharmacology. J. Ethnopharmacology, 117:1-27 DOI: https://doi.org/10.1016/j.jep.2008.01.025
Kanupriya, Madhavi Latha, P., Aswath, C., Laxman, R., Padmakar, B., Vasugi, C. and Dinesh, M.R. 2011. Cultivar Identification and Genetic Fingerprinting of Guava (Psidium guajava) using Microsatellite Markers. Int’l. J. Fr. Sci., 11:184-196 DOI: https://doi.org/10.1080/15538362.2011.578521
Khan, M.L.H. and Ahmad, J. 1985. A pharmacognostic study of Psidium guajava L. Int’l. J. Crude Drug Res., 23:95–103 DOI: https://doi.org/10.3109/13880208509069009
Lepitre, V., Nansot, G., Grangeon, R., Pomies, V., Rivallan, R., Risterucci, A.M., Valdés-Infante, J., Rodríguez- Medina, N.N., Muth, J., Boike, J., Prüfer, D., Becker, D., Rohde, W., Ritter, E. and Billotte, N. 2010. The Microsatellite (SSR)/AFLP Reference Linkage Map of Guava. Acta. Hort., 849:183-191 DOI: https://doi.org/10.17660/ActaHortic.2010.849.20
Nimisha, S., Kherwar, D., Ajay, K.M., Singh, B. and Usha, K. 2013. Molecular breeding to improve guava (Psidium guajava L.): Current status and future prospective. Sci. Hortic., 164:578-588 DOI: https://doi.org/10.1016/j.scienta.2013.10.017
Nogueira, M.A., Guilhen, H.S.J., Mangaravite, E., Ferreira, A. and Ferreira, F.S.M. 2012. Discriminant analysis of wild guava tree by morphological descriptors and microsatellites. In: Proceedings of 3rd International Symposium on Guava and other Myrtaceae, Petrolina, PE, Brazil. Session IV: Myrtaceae Biotechnologies and Genomics
Noia, L.R., Coser, S.M., Guilhen, J.H.S., Ferreira, A. and Ferreira, M.F.S. 2012. Genetic distance of guava genotypes from different altitudes by microsatellites. In: Proceedings of 3rd International Symposium on Guava and other Myrtaceae, Petrolina, PE, Brazil. Session IV: Myrtaceae Biotechnologies and Genomics
Ogundiwin, E., Peace, C.P., Gradziel, T.M., Parfitt, D.E., Bliss, F.A. and Crisosto, C.H. 2009. A fruit quality gene map of Prunus. BMC Genomics, 10:587, doi:10.1186/1471-2164-10-587 DOI: https://doi.org/10.1186/1471-2164-10-587
Oliveira, E.J., Vieir, M.L.C., Garcia, A.A.F., Munhoz, C.F., Margarido, G.R., Consoli, L., Matta, F.P., Moraes, M.C., Zucchi, M.I. and Fungaro, M.H.P. 2008. An integrated molecular map of yellow passion fruit based on simultaneous maximum-likelihood estimation of linkage and linkage phases. J. Am. Soc. Hort. Sci., 133:35-41 DOI: https://doi.org/10.21273/JASHS.133.1.35
Padmakar, B., Kanupriya, C., Madhavi Latha, P., Prashant, K.S., Dinesh, M.R., Sailaja, D. and Aswath, C. 2015. Development of SRAP and SSR marker based genetic linkage maps of guava (Psidium guajava L). Sci. Hortic., 192:158-165 DOI: https://doi.org/10.1016/j.scienta.2015.04.031
Pauly, L., Flajoulot, S., Garon, J., Julier, B., Béguier, V. and Barre, P. 2012. Detection of favorable alleles for plant height and crown rust tolerance in three connected populations of perennial ryegrass (Lolium perenne L.). Theor. Appl. Genet., 124:1139-1153 DOI: https://doi.org/10.1007/s00122-011-1775-5
Rai, K.M., Phulwaria, M., Gupta, K.M., Shekhawat, N.S. and Jaiswal, U. 2012. Genetic homogeneity of guava plants derived from somatic embryogenesis using SSR and ISSR markers. Plant Cell Tiss. Organ. Cult., 111:259-264 DOI: https://doi.org/10.1007/s11240-012-0190-1
Rai, M.K., Phulwaria, M. and Shekhawat, N.S. 2013. Transferability of simple sequence repeat (SSR) markers developed in guava (Psidium guajava L.) to four Myrtaceae species. Mol. Biol. Rep., 40:5067-5071, http://dx.doi.org/10.1007/s11033-013-2608-1 DOI: https://doi.org/10.1007/s11033-013-2608-1
Risterucci, A.M., Duval, M.F., Rohde, W. and Billotte, N. 2005. Isolation and characterization of microsatellite loci from Psidium guajava L. Mol. Ecol. Notes, 5:745-748 DOI: https://doi.org/10.1111/j.1471-8286.2005.01050.x
Risterucci, A.M., Nansot, G., Grangeon, R., Lepitre, V., de Reeper, A., Argout, X., Ruiz, M. and Billotte, N. 2010. Development of Guava Microsatellite (SSR) Markers Using the SAT Software. Acta. Hort., 849:113-119 DOI: https://doi.org/10.17660/ActaHortic.2010.849.12
Rodriguez, N., Valdés-Infante, J., Becker, D., Velazquez, B., Gonzalez, G., Sourd, D., Rodriguez, J., Billotte, N., Risterucci, A.M., Ritter, E. and Rohde, W. 2007. Characterization of guava accessions by SSR markers, extension of the molecular Linkage map, and mapping of QTLs for vegetative and reproductive characters. Acta. Hort., 735:201-215 DOI: https://doi.org/10.17660/ActaHortic.2007.735.27
Santos, C.A.F., Correa, L.C. and Costa, S.R. 2011. Genetic divergence among Psidium accession based on biochemical and agronomic variables. Crop Breed. Appl. Biotechnol., 11:149-156 DOI: https://doi.org/10.1590/S1984-70332011000200007
Serba, D., Wu, L., Daverdin, G., Bahri, B.A., Wang, X., Kilian, A., et al. 2013. Linkage maps of lowland and upland tetraploid switchgrass ecotypes. BioEnergy Res., 6:953-965 DOI: https://doi.org/10.1007/s12155-013-9315-6
Shruthi, S.D., Roshan, A., Timilsina, S.S. and Sunita, S. 2013. A review on the medicinal plant Psidium guajava Linn. (Myrtaceae). J. Drug Deliv. Therapeutics, 3:162-168 DOI: https://doi.org/10.22270/jddt.v3i2.404
Sudarshini, K.V., Padmakar, B., Manjunath, S.P., Lakshman, R. and Aswath, C. 2014. The genetic linkage maps of Anthurium species based on RAPD, ISSR and SRAP markers. Sci. Hortic., 178:132-137 DOI: https://doi.org/10.1016/j.scienta.2014.08.017
Valdés-Infante, D., Becker, D., Rodríguez, N., Velázquez, B., González, G., Sourd, D., Rodríguez, J., Ritter, E. and Rohde, W. 2003. Molecular characterization of Cuban accessions of guava (Psidium guajava L.): establishment of a first molecular linkage map and mapping of QTLs for vegetative characters. J. Genet. Breed., 57:349-358
Viji, G., Harris, D.L., Yadav, A.K. and Zee, F.T. 2010. Use of microsatellite markers to characterize genetic diversity of selected accessions of guava (Psidium guajava L.) in the United States. Acta. Hort., 859:169-176 DOI: https://doi.org/10.17660/ActaHortic.2010.859.20
Wang, S., Basten, C.J. and Zeng, Z.B. 2010. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, USA. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm
Zhang, R., Wu, J., Li, X., Awais Khan, M., Chen, H., Korban, S.S. and Zhang, S. 2013. An AFLP, SRAP, and SSR genetic linkage map and identification of QTLs for fruit traits in pear (Pyrus L.) Plant Mol. Biol. Repr., 31:678-687 DOI: https://doi.org/10.1007/s11105-012-0544-1
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Copyright (c) 2015 B Padmakar, D Sailaja, C Aswath (Author)
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