Studies on genetic variability and relationship of bael (Aegle marmelos (L) Correa) using morphological and molecular markers
Keywords:Bael, genetic variability, morphology, molecular markers
Bael (Aegle marmelos (L) Correa) is an important underutilized fruit crop of India. A total of 25 bael trees were selected from 356 bael trees of Sakharayapattana in Chikkamagalur district, Karnataka, India based on the fruit morphological traits (fruit weight, pulp weight, skull thickness, seed weight per fruit, No. of seeds per fruit, No. of locules per fruit, No. of seeds per locule, pulp wt. : seed wt.). These 25 trees were evaluated for phenotypic and genotypic variations using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. RAPD and ISSR markers showed significant polymorphism among the trees. Jaccard’s genetic similarity value of RAPD and ISSR was found in the range of 0.00–0.95 and 0.06–0.56, respectively suggesting a moderate level of genetic diversity. The present study revealed that molecular markers can be successfully utilized for determining genetic diversity and relationship of bael trees for further varietal improvement.
2. Benharrat, H., Veronesi, C., Theodet, C. and Thalouam, P. 2002. Orobanche species and population discrimination using inter simple sequence repeat (ISSR). Weed Res., 42 : 470–474.
3. Mujeeb, F., Bajpai, P., Pathak, N. and Verma, S.R. 2017. Genetic diversity analysis of medicinally important horticultural crop Aegle marmelos by ISSR markers. Methods mol. biol., 1620:195-211.
4. Virk, P.S., Zhu, J., Newburg, H.J., Bryan, G.J., Jeckson, M.T. and Ford-Lloyd, B.V. 2001. Effectiveness of different classes of molecular markers for classifying and revealing variation in rice germplasm. Euphytica, 112 :275–284.
5. Fernandez, M.E., Figueiras, A.M. and Benito, C. 2002. The use of ISSR and RAPD markers for detecting DNA polymorphisms, tree identification and genetic diversity among barley cultivars with known origin. Theor. Appl. Gen., 104 :845–851.
6. Pandey, D., Shukla, S.K. and Kumar, A. 2008. Variability in bael accessions from Bihar and Jharkhand. Indian J. Hort., 65 :226-229.
7. Pandey, D., Tandon, D.K., Hudedamani, U. and Tripathi, M. 2013. Variability in bael (Aegle marmelos Corr.) trees from eastern Uttar Pradesh. Indian J. Hort., 70:170-178.
8. Mitra, S.K., Maity, C.S., Mani, D. and Ghosh, B. 2010. Genetic resources of bael (Aegle marmelos Correa) – a potential underutilized fruit. Acta Hort., 864:49-52.
9. Singh, V.P. and Misra, K.K. 2010. Analysis of genetic variability and heritability in bael (Aegle marmelos Correa) germplasm. Prog. Agric., 10 :132-134.
10. Diallo, B.O., Joly, H.I., Mckey, D., Mckey, M.H. and Chevalier, M.H. 2007. Genetic diversity of Tamarindus indica populations: Any clues on the origin from its current distribution. Afr. J. Biotechnol., 6: 853-860.
11. Nayak, D., Singh, D.R., Sabarinathan, P., Singh, S. and Nayak, T. 2013. Random amplified polymorphic DNA (RAPD) markers reveal genetic diversity in bael (Aegle marmelos Correa) trees of Andaman Islands, India. Afr. J. Biotechnol., 12:6055-6060.
12. Singh, S., Singh, D.R., Faseela, F., Kumar, N., Damodaran, V. and Srivastava, R.C. 2012. Diversity of 21 taro (Colocasia esculenta L. Schott) trees of Andaman Islands. Genet. Resour. Crop Evo., 59: 821-829.
13. Rajwana, I.A., Tabbasam, N., Malik, A.U. and Malik, A.S. 2008. Assessment of genetic diversity among mango (Mangifera indica L.) trees using RAPD markers. Sci. Hort., 117 :297-301.
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Copyright (c) 2022 Amulya R N, Nagarajappa Adivappar, Shivakumar B S, Satish K M
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