Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.)

Authors

  • Jessy Mol K K Author
  • Lakshmana Reddy D C Author
  • Manoj Y B Author
  • Madhavi Reddy K Author

DOI:

https://doi.org/10.24154/jhs.v15i1.783

Keywords:

CGMS, Hot pepper, Marker Assisted Selection, Mitochondria, ORF

Abstract

Existence of CGMS system in hot pepper is due to the rearrangements in the mitochondrial genome and is largely used in economized and pure F 1 hybrid seed production around the world. The orf456, a new ORF present at flanking region of the coxII gene at the 3’ end, was distinguished male sterile cytoplasm in hot peppers along with atp6-2gene. In the current study, eighteen pepper genotypes (nine each of A and corresponding B lines) of varied origin were used to validate with two male sterile cytoplasm (S-cytoplasm) specific sequence characterised amplified region (SCAR) markers viz., atp6-2 (875 bp) and orf456 (456 bp) and one restoration-of-fertility (Rf) locus specific marker, CRF (550 bp). The results clearly showed that the presence of CMS-S-cytoplasm and absence of restoration-of-fertility (Rf) gene in the pepper genotypes studied and is comparable with the phenotypic data. In view of the outcomes it has been reasoned that the accessible S and Rf markers available in the public domain are reproducible and can be promptly utilized for marker assisted selection (MAS) in hot pepper crop improvement program.

References

Budar F and Pelletier G (2001) Male Sterility in Plants; Occurrence, Determinism, Significance and Use. Life Science 324: 543-550.

Doyle J J, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15.

Grelon M, Budar F, Bonhomme S, Pelletier G (1994) Ogura cytoplasmic male-sterility (CMS)- associated orf138 is translated into a mitochondrial membrane polypeptide in male sterile Brassica cybrids. Molecular Genetics and Genomics 243:540–547

Gulyas G, Pakozdi K, Lee JS, Hirata Y (2006) Analysis of fertility restoration by using cytoplasmic male sterile red pepper (Capsicum annuum L.) lines. Breeding Science 56:331– 334.

Hanson MR (1991) Plant mitochondrial mutations and malesterility. Annual Review of Genetics 25:461–486

Hanson M and Bentolila S (2004) Interactions of mitochondrial andnuclear genes that affect male gametophyte development.Plant Cell 16:S154- S169

Ji J J, Huang W, Yin C, Gong Z H (2013) Mitochondrial cytochrome c oxidase and F1Fo- ATPase dysfunction in peppers (Capsicum annuum L.) with cytoplasmic male sterility and its association with orf507 and atp6-2genes. International Journal Molecular Science 14:1050–1068.

Kim DH, Kim BD (2005) Development of SCAR markers for early identification of cytoplasmic male sterility genotype in chili pepper (Capsicum annuum L.). Molecules & Cells 20:416–422.

Kim DS, Kim DH, Yoo JY, Kim BD (2006) Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper (Capsicum annuum L.). Molecules & Cells 21:135–140.

Kim DH, Kang JG, Kim BD (2007) Isolation and characterization of the cytoplasmic male sterility-associated orf456 gene of chili pepper (Capsicum annuum L.). Plant Molecular Biology 63:519–532.

Kumar R, Kumar S, Dwivedi N, Kumar S, Rai A, Singh M, et al., (2009) Validation of SCAR markers, diversity analysis of male sterile (S-) cytoplasm and isolation of an alloplasmic S- cytoplasm in Capsicum. Scientia Horticulturae. 120:167–172

Miller I and Bruns E (2016) The effect of disease on the evolution of females and the genetic basis of sex in populations with cytoplasmic male sterility. Proceedings. Biological Sciences. 283 : 20153035

Peterson P A (1958) Cytoplasmically inherited male sterility in Capsicum americana. Naturalist 92:111–119.

Pruitt K D and M R Hanson (1989) Cytochrome oxidase subunit II sequences in Petunia mitochondria: Two intron-containing genes and an intron-less pseudogene associated with cytoplasmic male sterility. Current Genetics. 16:281-291.

Pruitt K D and M R Hanson. (1991) Splicing of the Petunia cytochrome oxidase subunit II= intron.Current Genetics. 19:191 197

Reddy MK., Sadashiva, A T, Deshpande, A A, (2002) Cytoplasmic male sterility in chilli (Capsicum annuum L.). Indian Journal of Genetics. 62:363–364

Schnable P S and Wise R P (1998) The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Science 3:175- 180.

Zhang B X, Huang S W, Yang G M, Guo J Z (2000) Two RAPD markers linked to a major fertility restorer gene in pepper. Euphytica 113:155- 161.

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Published

30-06-2020

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Section

Original Research Papers

How to Cite

K K, J. M., D C, L. R. ., Y B , M., & K, M. R. . (2020). Validation of Molecular Markers Genetically Linked to S-Cytoplasm and Restoration-of-fertility (Rf) Loci in Hot Pepper (Capsicum annuum L.). Journal of Horticultural Sciences, 15(1), 52-61. https://doi.org/10.24154/jhs.v15i1.783

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