Studies on Physiological and Biochemical Changes in Relation to Seed Viability in Aged Onion Seeds
DOI:
https://doi.org/10.24154/jhs.v1i1.665Keywords:
Onion, Accelerated Ageing, Protein Profiles, EnzymesAbstract
Rapid loss in viability of onion seeds during seed storage is a major problem. Not much information concerning the physiological and biochemical changes is available. In the present investigations, seeds were aged artificially by exposure to 45°C+75% RH for a period of fifteen days. Samples were collected at three day intervals and physiological and biochemical changes in the aged seeds were compared to those in fresh seeds. Results revealed that ageing affected seed viability and vigour significantly and this effect was more pronounced with increase in duration of exposure to artificial ageing. Marked reduction in germination to an extent of 4,16 and 75% was noticed in three, six and nine day artificially aged (DAA) seeds, respectively, when compared to fresh seeds. Further increase in ageing duration to twelve and fifteen days resulted in total loss of germination. Increase in ageing duration decreased a amylase and dehydrogenase activities but increased peroxidase activity up to nine days of ageing. Lipid peroxidation increased consistently with increase in duration of ageing. At 15 DAA, 26.2% increase in malondialdehyde content over the control was observed. SDS PAGE protein profile and esterase zymograms of aged seeds showed alteration in banding pattern when compared to that of fresh seeds.
References
Agarwal, P.K. and Dadlani, M.1987. Techniques in seed science and technology. South Asian Publishers, New Delhi.
Aung, U.T. and McDonald, M.B. 1995. Changes in esterase activity associated with peanut {Arachis hypogea L.) seed deterioration. Seed Sci. & TechnoL, 23:101-111
Bernheim,F., Bernheim, M.L.C. and Wilbur, K.M. 1948. The reaction between thiobarbituric acid and the oxidation products of certain lipids. J. Biochem., 174:257-264
Chen, X.L., Chen, S.P. and Lu, X.X.1999. Comparative study on artificial ageing methods in evaluating the storability of rape {Brsassica napus L.) seeds. Acta Agron. Sin., 25:265-68.
Choudhuri, N. and Basu, R.N. 1988. Maintenance of seed vigour and viability in onion {Allium cepa L.). Seed Sci. & TechnoL, 16:51-61
Dubois. M., Gilles, K., Hamilton, J.K., Rebers, P.A. and Smith, F. 1951. A colorimetric method for determination of sugars. Nature, 168:167
ISTA (1999). International rules for seed testing. Seed Sci. & TechnoL, 13:299-520
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T^. Nature, 227:680-685
Lowry, H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193:265-75
McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. SeedScL & TechnoL, 21:111-2il
Mugnisjah, W.Q. and Nakamura, S. 1986. Methanol and ethanol stress for seed vigour evaluation in soybean. SeedScL & TechnoL, 14:95-103
Nandi, S., Ganguly, S. and Sen-mandi, S.1995. Seed technology for cold tolerance in rice. Int'l Rice Res. Notes, 20:20-21
Nautiyal, A.R., Thaphya, A.P and Purohit, A.N. 1985. Seed viability in Sal IV. Protein changes accompanying loss of viability in Shorea robusta L. Seed Sci. & TechnoL, 13:83-96
Pammenter, N.W., Adamson J.H. and Berjak, P. 1974. Viability of stored seed extension by cathodic protection. Science, 186:1123-1124
Pause, V.G. and Sukhatme, P.V. 1978. Statistical methods for agricultural workers. ICAR, New Delhi.
Roberts, E.H. 1981. Physiology of ageing and its application to drying and storage. Seed ScL & TechnoL, 9:359-372
Rudrapal, A.B. and Basu, R.N. 1979. Physiology of hydration dehydration treatment in the maintenance of seed viability in wheat. Ind. J. Exp. BioL, 17:768-771
Sadasivam, S. and Manickam, 1996. Biochemical methods. 2"^ Ed. New Age International Publ. Pvt. Ltd., New Delhi.
Wilson, D.O.Jr. and McDonald, M.B.Jr.l986. The lipid peroxidation model of seed ageing. Seed Sci. & TechnoL, 18:269-300
Downloads
Published
Issue
Section
License
Copyright (c) 2006 K Bhanuprakash, H S Yogeesha, L B Naik, M N Arun (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
