Studies on mutagenic sensitivity of seeds of pummelo (Citrus maxima Merr.)

M Sankaran; D Kalaivanan; Suni Gowda

doi:10.24154/jhs.v16i1.1127

Authors

M Sankaran ICAR-Indian Institute of Horticultural Research Author
D Kalaivanan ICAR-Indian Institute of Horticultural Research Author
Suni Gowda ICAR-Indian Institute of Horticultural Research Author

DOI:

https://doi.org/10.24154/jhs.v16i1.1127

Keywords:

Gamma Irradiation, Germination, Gray (Gy) LD50, Mutation, Pummelo

Abstract

Mutation breeding is a key method of generating a large number of heritable variations. Effective dose (LD50) needs to be standardized for inducing sufficient variation in a crop. In the present study, seeds were irradiated with different doses of Gamma rays and found that 66.94 Gy could suppress germination close to 50 percent (LD50) in pummelo. This 60 Gy gamma dose can effectively be used for raising the mutant populations to identify a desirable mutation in pummelo.

Downloads

Download data is not yet available.

References

Dhatt, A S., Rattanpal, H. S., Khokhar, R. and Singh, S. N. 2000. Standardising LD50 dose of gamma rays and EMS in kinnow seeds. Journal of Research Punjab Agricultural University, 37 (3-4): 194–7.

Guadagni, D.G., Maier, V.P and Turnbaugh, J.G, 1973. Effects of some citrus constituents on taste thresholds for limonin and naringin bitterness. J. Sci. Food Agric., 24: 1199-1205.

Gulsen, O., Uzun, A., Pala, H., Canihos, E. and Kafa, G. 2007. Development of seedless and mal secco tolerant mutant lemons through bud wood irradiation. Scientia Horticulturae, 112: 184-190.

Kafa, G., Seday, U., Uysa,l O. and Polatoz, S. 2015. Effect of budwood irradiation on seed number of ‘Clementine’, ‘Nova’ and ‘Robinson’ mandarin mutants. Proc. XII Intl. Citrus Congress. Acta Horticulturae No. 1065, International Society for Horticultural Science, Leuven, Belgium.

Kulkarni, G. B. 2011. Effect of mutagen on pollen fertility and other parameters in horse gram (Macrotylomauni florum (Lam.) Verdc). Bio. Sci. Discovery, 2 (1): 146-150.

Mishra, P. J., Ganapathi, T. R., Suprasanna, P., Bapat, V. A. 2007. Effect of Single and recurrent gamma irradiation on in vitro shoot cultures of banana. Int. J. Fruit Sci.,7:45-57.

Latado, R. R., TulmannNeto, A., Ando A Iemma, A., F, Pompeu Junior, J., Figueiredo, J. O., Pio, R. M., Machado, M. A., Namekata, T., Ceravolo, L. and Rossi, A. C. 2001. Sweet orange ‘Pera’ mutants with low number of seeds obtained

through mutation induction. Revista Brasileira de Fruticultura, 23 (2):339–44.

Maluszynski, M., Nichterlein, K., Vanzanten, L. and Ahloowalia, B. S. 2000. Officially released mutant varieties – the FAO/IAEA database. Mutation Breeding Review, 12: 1–84.

Mariana, B., Arisah, H., Yenni and Selvawajayanti. M. 2018. Seedless fruit pummelo induced by Gamma Ray irradiation: Fruit morphological characters and stability evaluation. Biodiversitas. 19(2): 706-711.

Mongkolkul, P., Rodart, P., Pipatthitikorn, T., Meksut, L. and Sanguandeekul, R. 2006 Debittering of Tangerine (Citrus reticulata Blanco.) Juice by ß-Cyclodextrin polymer. J Incl Phenom Macrocycl Chem., 56:167– 170.

Montanola, M. J., Galaz, A., Gambardella, M. and Mártiz, J. 2015. New low seeded mandarin (Citrus reticulata) and lemon (C. limon) selections obtained by Gamma irradiation. Proc. XII Intl. Citrus Congress. Acta Horticulturae No. 1065, International Society for Horticultural Science, Leuven, Belgium.

Sanada, T. and Amano, E. 1998. Induced mutation in fruit trees. In Jain, SM., Brar, D. S. and Ahloowalia, B. S. Soma clonal variation and induced mutations in crop improvement. Kluwer Academic Publishers. England. p. 401-419.

Singh, B.D. 1994. Mutations in crop improvement In Plant Breeding Kalyani Publ., Ludhiana p 434-450

Sinha, S. S and Godward, N. 1972. Radiation studies in Lens culanaris. Induction of mutations and type of mutants. J. Cytol. Genet, 8: 131- 136.

Sjodin, J. 1962. Some observations in X1 and X2 of Vicia faba (L.) after treatment with different mutagens. Hereditas, 48: 565-586.

Smith, M. K., Hamill, S. D., Langdon, P. W., Giles, J. E., Doogan, V. J. and Pegg, K. G. 2006. Towards the development of a Cavendish banana resistant to race 4 of Fusarium wilt: gamma irradiation of micro propagated Dwarf Parfitt (Musa spp., AAA group, Cavendish subgroup). Aus. J. Exp. Agric., 46(1):107-113.

Sparrow, A. K., Rogers, A. F. and Schwenmer, S. S. 1968. Radio sensitivity studies which woody plants. I. Acute gamma irradiation survival data for 28 species and predictions for 190 species. Radiation Botany, 8:149-186.

Spiegel-Roy, P., Vardi, A., Yaniv, Y., Fanberstein, L., Elhanati, A. and Carmi, N. 2007. ‘Ayelet’ and ‘Galya’: New seedless lemon cultivars. Hort science, 42(7): 1723-1724.