Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.)

Authors

  • B Padmakar Author
  • C Kanupriya Author
  • P Madhavi Latha Author
  • C Vasugi Author
  • M R Dinesh Author
  • D Sailaja Author
  • C Aswath Author

DOI:

https://doi.org/10.24154/jhs.v11i1.96

Keywords:

Composite Interval Mapping, Guava, Linkage Map, Pseudo-Testcross, Quantitative Trait Loci (QTL)

Abstract

The present research focuses mainly on molecular mining and morphological evaluation of guava genome within a full-sib population and, thereby, mapping of quantitative trait loci related to fruit quality traits, viz., seed strength (hardness/softness) and average fruit weight. Linkage maps were enriched for both parental lines, 'Kamsari' and 'Purple Local' using a set of 60 RAPD markers following the pseudo-testcross strategy on a panel of 94 progeny. A total of 480 scorable markers were identified, of which 131 were specific to 'kamsari' and 28 to 'Purple Local', segregating as test cross markers, and, 321 showing intercross pattern common to both. 'Kamsari' spanned a total length of 1959.1cM with average marker interval distance of 3.93cM, while 'Purple Local' spanned a length of 1537.9cM with average marker interval distance of 3.29cM, by forming 11 linkage groups. Estimated genome length observed was 93.02% and 92.77% in 'Kamsari' and 'Purple Local', respectively. Composite Interval Mapping (CIM) was computed at significance of 0.05 and LOD threshold greater than 3.0, which led to detection of one major QTL for the trait of average fruit weight, and, four QTLs for the trait of seed strength (hardness/softness). Of these, two were major and two minor QTLs. Our study provides molecular mapping information on marker-assisted selection for improvement of guava in a breeding program.

References

Ahmed, B., Mannan, M.A. and Hossain, S.A. 2011. Molecular characterization of guava (Psidium guajava L.) germplasm by RAPD analysis. Int’l. J. Nat. Sci., 1:62-67

Chakravarti, A., Lasher, L.K. and Reefer, J.E. 1991. A maximum-likelihood method for estimating genome length using genetic linkage data. Genetics, 128:175-182

Chandra, R. and Mishra, M. 2007. Biotechnological interventions for improvement of guava (Psidium guajava L.). Acta Hort., 735:117-125

Chen, T.W., Ng, C.C., Wang, C.Y. and Shyu, Y.T. 2007. Molecular identification and analysis of Psidium guajava L. from indigenous tribes of Taiwan. J. Food Drug Anal., 15:82-88

Dahiya, K.K., Archak, S. and Karihaloo, J.L. 2002. DNA fingerprinting of guava (Psidium guajava L.) cultivars using RAPD markers. Indian J. Pl. Genet. Res., 15:112–115

Dinesh, M.R. and Vasugi, C. 2010. Phenotypic and genotypic variations in fruit characteristics of guava (Psidium guajava). Indian J. Agril. Sci., 80:998–999

Fishman, L., Kelly, A.J., Morgan, E. and Willis, J.H. 2001. A genetic map in the Mimulus guttatus species complex reveals transmission ratio distortion due to heterospecific interactions. Genetics, 159:1701-1716

Grattapaglia, D. and Sederoff, R. 1994. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics, 137:1121-1137

Kanupriya, C., Madhavi Latha, P., Aswath, C., Laxman, R., Padmakar, B., Vasugi, C. and Dinesh, M.R. 2011. Cultivar identification and genetic fingerprinting of guava (Psidium guajava) using microsatellite markers. Int’l. J. Fruit Sci., 11:184-196

Lepitre, V., Nansot, G., Grangeon, R., Pomies, V., Rivallan, R., Risterucci, A.M., Valdés- Infante, J., Rodríguez- Medina, N.N., Muth, J., Boike, J., Prüfer, D., Becker, D., Rohde, W., Ritter, E. and Billotte, N. 2010. The microsatellite (SSR)/AFLP reference linkage map of guava. Acta Hort., 849:183-191

Li, G. and Quiros, C.F. 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet., 103:455-461

Lu, J.J., Zhao, H.Y., Suo, N.N., Wang, S., Shen, B., Wang, H.Z. and Liu, J.J. 2012. Genetic linkage maps of Dendrobium moniliforme and D. officinale based on EST-SSR, SRAP, ISSR and RAPD markers. Sci. Hortic., 137:1-10

Maliepaard, C., Jansen, J. and Van Ooijen, J.W. 1997. Linkage analysis in a full-sib family of an outbreeding plant species: overview and consequence for applications. Genet. Res., 70:235-250

Nakasone, H.Y. and Paull, R.E. 1998. Tropical Fruits: Crop production science in horticulture. CAB International, Wallingford, UK, pp 468

Nimisha, S., Kherwar, D., Ajay, K.M., Singh, B. and Usha, K. 2013. Molecular breeding to improve guava (Psidium guajava L.): Current status and future prospective. Sci. Hortic., 164:578-588

Padmakar, B., Kanupriya, C., Madhavi Latha, P., Prashant, K.S., Dinesh, M.R., Sailaja, D. and Aswath, C. 2015a. Development of SRAP and SSR marker based genetic linkage maps of guava (Psidium guajava L). Sci. Hortic., 192:158-165

Padmakar, B., Sailaja, D. and Aswath, C. 2015b. Molecular exploration of guava (Psidium guajava L.) genome using SSR and RAPD markers: a step towards establishing linkage map. J. Hortl. Sci., 10:130-135

Pessanha, P.G.D.O., Viana, A.P., Júnior, A.T.D.A., Souza, R.M.D., Teixeira, M.C. and Pereira, M.G. 2011. Assessment of genetic diversity in access to Psidium spp. via RAPD markers. Rev. Bras. Frutic., 33:129-136

Prakash, D.P., Narayanaswamy, P. and Sondur, S.N. 2002. Analysis of molecular diversity in guava using RAPD markers. J. Hort. Sci. Biotechnol., 77:287–293

Risterucci, A.M., Duval, M.F., Rohde, W. and Billotte, N. 2005. Isolation and characterization of microsatellite loci from Psidium guajava L. Mol. Ecol. Notes, 5:745-748

Risterucci, A.M., Nansot, G., Grangeon, R., Lepitre, V., de Reeper, A., Argout, X., Ruiz, M. and Billotte, N. 2010. Development of guava microsatellite (SSR) markers using the SAT software. Acta Hort., 849:113-119

Ritter, E., Rodríguez-Medina, N.N., Velásquez, B., Rivero, D., Rodríguez, J.A., Martínez, F. and Valdés-Infante, J. 2010. QTL (Quantitative Trait Loci) analysis in guava. Acta Hort., 849:193-202

Rodriguez, N., Valdés-Infante, J., Becker, D., Velazquez, B., Gonzalez, G., Sourd, D., Rodriguez, J., Billotte, N., Risterucci, A.M., Ritter, E. and Rohde, W. 2007. Characterization of guava accessions by SSR markers, extension of the molecular linkage map, and mapping of QTLs for vegetative and reproductive characters. Acta Hort., 735:201-215

Sharma, A.S., Sehrawat, S.K., Singhrot, R.S. and Boora, K.S. 2007. Assessment of genetic diversity and relationship among Psidium spp. through RAPD analysis. Acta Hort., 735:71-78

Shruthi, S.D., Roshan, A., Timilsina, S.S. and Sunita, S. 2013. A review on the medicinal plant Psidium guajava Linn. (Myrtaceae). J. Drug Deliv. Therapeutics, 3:162-168

Sokal, R.R. and Rohlf, F.J. 1981. Biometry: the Principles and Practice of Statistics in Biological Research, W.H. Freeman and Company, San Francisco, USA

Tanksley, S.D., Ganal, M.W., Prince, J.P., Devincente, M.C., Bonierbale, M.W., Broun, P., Fulton, T.M., Giovanonni, J.J., Grandillo, S., Martin, G.B., Messeguer, R., Miller, J.C., Miller, L., Paterson, A.H., Pineda, O., Roder, M.S., Wing, R.A., Wu, W. and Young, N.D. 1992. High density molecular linkage maps of tomato and potato genomes: biological interferences and practical applications. Genetics, 132:1141–1160

Trapletti, A. and Hornik, K. 2007. Tseries: time series analysis and computational finance. R package version 0.10-11

Valdés-Infante, D., Becker, D., Rodríguez, N., Velázquez, B., González, G., Sourd, D., Rodríguez, J., Ritter, E. and Rohde, W. 2003. Molecular characterization of Cuban accessions of guava (Psidium guajava L.), establishment of a first molecular linkage map and mapping of QTLs for vegetative characters. J. Genet. Breed., 57:349-358

Wang, S., Basten, C.J. and Zeng, Z.B. 2010. Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, USA.

Wen, M., Wang, H., Xia, Z., Zou, M., Lu, C. and Wang, W. 2010. Development of EST-SSR and genomic-SSR markers to assess genetic diversity in Jatropha curcas L. BMC Res. Notes, 3:42, doi: 10.1186/1756-0500-3-42.

Xie, W., Zhang, X., Cai, H., Huang, L., Peng, Y. and Ma, X. 2011. Genetic maps of SSR and SRAP markers in diploid orchard grass (Dactylis glomerata L.) using the pseudo-testcross strategy. Genome, 54:212-221

Zeng, Z.B. 1994. Precision mapping of quantitative trait loci. Genetics, 136:1457-1468

Zhang, F., Chen, S., Chen, F., Fang, W., Chen, Y. and Li, F. 2011. SRAP-based mapping and QTL detection for inflorescence-related traits in chrysanthemum (Dendranthema morifolium). Mol. Breeding, 27:11-23

Zhang, R., Wu, J., Li, X., Awais Khan, M., Chen, H., Korban, S.S. and Zhang, S. 2013. An AFLP, SRAP, and SSR genetic linkage map and identification of QTLs for fruit traits in pear (Pyrus L.). Pl. Mol. Biol. Rep., 31:678-687

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Published

30-06-2016

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Original Research Papers

How to Cite

Padmakar, B., Kanupriya, C., Latha, P. M., Vasugi, C., Dinesh, M. R., Sailaja, D., & Aswath, C. (2016). Enrichment of Genetic Linkage Maps and Mapping QTLs Specific to Seed Strength-Hardness/Softness-In Guava (Psidium guajava L.). Journal of Horticultural Sciences, 11(1), 13-20. https://doi.org/10.24154/jhs.v11i1.96

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