Assessment of in vitro alpha-glucosidase inhibitory activity, phenolics, flavonoidsand antioxidant potential of jamun (Syzygium cumini) seeds
DOI:
https://doi.org/10.24154/jhs.v19i1.2837Keywords:
alpha-glucosidase, antioxidant potential, diabetes, flavonoids, jamun, phenolicsAbstract
Jamun (Syzygium cumini Linn. Syn. Eugenia jambolana) genotypes collected from different parts of the country were evaluated for in vitro alpha-glucosidase inhibitory activity, polyphenolic content and antioxidant potential along with fruit characters. Results indicated significant variation among genotypes for fruit characters, phenolics, flavonoids, antioxidant potential, and alpha-glucosidase inhibitory potential. Total phenolics and flavonoids in seeds varied between 15.5 mg to 98.7 mg GAE/g and 0.79 mg to 9.03 mg CE/g seeds, respectively. The seeds with a high content of polyphenolic compounds required a very less amount of sample to inhibit alpha-glucosidase enzyme. The genotype Selection 45 recorded the highest alpha-glucosidase inhibitory activity, and required only 50.3 µg of sample to inhibit 50 % of the enzyme activity. Both phenolics and flavonoids content positively correlated with antioxidant potential and negatively correlated with enzyme inhibitory potential. From cluster analysis, the genotypes AJG-85, Kaithanal, Selection-45, Collection-2, and Konkan Bahdoli were identified as superior genotypes having a considerable amount of seeds with alpha glucosidase inhibitory compounds. These genotypes/collections may be effectively utilized for further studies to isolate and characterize the bioactive constituents with alpha-glucosidase inhibitory potential.
References
Arivalagan, M., Priyanka D. R., & Rekha, A. (2021). Optimization of methodology for the extraction of polyphenolic compounds with antioxidant potential and α-glucosidase inhibitory activity from jamun (Syzygium cumini L.) seeds. Journal of Horticultural Science, 16(1), 26-35. https://doi.org/10.24154/JHS.2021.v16i01.003
Arivalagan, M., Roy, T. K., Yasmeen, A. M., Pavithra, K. C., Jwala, P. N., Shivasankara, K. S., Manikantan, M. R., Hebbar, K. B., & Kanade, S.R. (2018). Extraction of phenolic compounds with antioxidant potential from coconut (Cocos nucifera L.) testa and identification of phenolic
acids and flavonoids using UPLC coupled with TQD-MS/MS. LWT - Food Science and Technology, 92, 116–126. https://doi.org/10.1016/j.lwt.2018.02.024
Ayya, N., Nalwade, V., & Khan, T. N. (2015). Effect of jamun (Syzygium cuminiL.) seed powder supplementation on blood glucose level of typeII diabetic subject. Food Science Research Journal, 6(2), 353-356. https://doi.org/10.15740/HAS/FSRJ/6.2/353-356
Benzie, I. E. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ‘antioxidant power’: The FRAP assay.
Analytical Biochemistry, 239, 70–76. https://doi.org/10.1006/abio.1996.0292
Bhatia, I.S., &Bajaj, K.L. (2005). Chemical constituents of the seeds and bark of Syzigium cumini fruit. Food chemistry, 90, 727-733.
https://doi.org/10.1055/s-0028-1097868
Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. LWT-Food Science and
Technology, 28, 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
Bushra, S., Farooq, A., & Roman, P. (2007). Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica and Eugenia jambolana Lam trees. Food Chemistry, 104, 1106-1114. https://doi.org/10.1016/j.foodchem.2007.01.019
Ghosh, P., Pradhan, R. C., Mishra, S., Patel, A. S., & Kar, A. (2017). Physicochemical and Nutritional Characterization of Jamun
(Syzygium Cuminii). Current Research in Nutrition and Food Science, 5(1), 25-35. https://dx.doi.org/10.12944/CRNFSJ.5.1.04
JMP (2012). JMP® 10.0.2. Statistical analysis software system. Cary, NC, USA: SAS Institute.
Katiyar D, Singh V, & Ali M. (2016). Recent advances in pharmacological potential of Syzygium cumini: A review. Advances in Applied Science Research, 7(3),1-2.
SAS. (2012). Statistical analysis software system, version 9.3. Cary, NC, USA: SAS Institute.
Singleton, V. L., Orthofer, R., & Lamuela-Raventos, R. M. (1999). Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology, 299, 152-178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1
Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559. https://doi.org/10.1016/S0308-8146(98)00102-2
Downloads
Published
Issue
Section
License
Copyright (c) 2024 M Arivalagan, D R Priyanka, A Rekha, P C Tripathi (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
