Evaluation of pre-treatments for shelf life extension of minimally processedfenugreek (Trigonella foenum graecum L.)
DOI:
https://doi.org/10.24154/Keywords:
Calcium chloride, fenugreek leaves, kinetin, minimal processing, pretreatment, sanitizerAbstract
Potential of different pretreatments for shelf life enhancement and nutritional quality maintenance in minimally processed fenugreek (Trigonella foenum-graecum) leaves (MPFL) at 8°C storage were evaluated. Pretreatment agents such as calcium propionate, calcium chloride, kinetin, gibberellic acid, 1-methyl cyclo propene, neutral electrolysed water and sodium hypochlorite were used. The results have proven that a five minutes dip in kinetin solution (50 mg L-1) was the best among all tested pretreatments; and it resulted in achieving eight days shelf life compared to four days in control samples. Besides this, better maintenance of total antioxidants (1735 mg ascorbic acid equivalent antioxidant capacity kg-1) and proteins (44 g kg-1), at levels close to that of fresh leaves, could be achieved. Calcium propionate (1.5 g L-1) was identified as the second best treatment. It was also shown that minimally processed fenugreek leaves (MPFL) is a rich source of vitamins B1, B2, E and K. The B vitamins were susceptible to deterioration during storage, while their loss could be minimized with kinetin treatment. Sanitizers like chlorine (100 mg L-1 available chlorine) and neutral electrolysed water (100 and 200 mg L-1) have reduced the shelf life of MPFL indicating their phytotoxicity to FL. The results of the study open up the possibility of using kinetin as an effective pretreatment tool for MPFL in minimal processing industry.
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Ahmad, A., Alghamdi, S. S., Mahmood, K., & Afzal, M. (2016). Fenugreek: A multipurpose crop—Potentialities and improvements. Saudi Journal of Biological Sciences, 23(2), 300–310. https://doi.org/10.1016/j.sjbs.2015.09.015
Al Ubeed, H. M. S., Wills, R. B. H., Bowyer, M. C., & Golding, J. B. (2018). Comparison of hydrogen sulphide with 1-methylcyclopropene (1-MCP) to inhibit senescence of the leafy vegetable, pak choy. Postharvest Biology and Technology, 137, 129–133. https://doi.org/10.1016/j.postharvbio.2017.11.020
AOAC. (1995a). Carotene in fresh plant materials and silages. In Official methods of analysis of the Association of Official Analytical Chemists (Method No. 941.15; K. Helrich, Ed.). AOAC Inc.
AOAC. (1995b). Chlorophyll in plants. In Official methods of analysis of the Association of Official Analytical Chemists (Method No. 942.04; K. Helrich, Ed.). AOAC Inc.
Artés, F., & Allende, A. (2005). Minimal fresh processing of vegetables, fruits and juices. In D.-W. Sun (Ed.), Emerging technologies for food processing (pp. 677–716). Academic Press. https://doi.org/10.1016/B978-012676757-5/50028-1
Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292
Cefola, M., Amodio, M. L., Rinaldi, R., Vanadia, S., & Colelli, G. (2010). Exposure to 1-methylcyclopropene (1-MCP) delays the effects of ethylene on fresh-cut broccoli raab (Brassica rapa L.). Postharvest Biology and Technology, 58(1), 29–35. https://doi.org/10.1016/j.postharvbio.2010.05.001
Food Data Central. (2022, May 6). United States Department of Agriculture. https://fdc.nal.usda.gov/
Ghimire, U., Abeli, P., Brecht, J. K., Pliakoni, E., & Liu, T. (2024). Unique molecular mechanisms revealed for the effects of temperature, CA, ethylene exposure, and 1-MCP on postharvest senescence of broccoli. Postharvest Biology and Technology, 213, 112919. https://doi.org/10.1016/j.postharvbio.2024.112919
Hassan, F. A. S., & Mahfouz, S. A. (2012). Effect of 1-methylcyclopropene (1-MCP) on the postharvest senescence of coriander leaves during storage and its relation to antioxidant enzyme activity. Scientia Horticulturae, 141, 69–75. https://doi.org/10.1016/j.scienta.2012.04.021
Himanen, M., Prochazka, P., Hanninen, K., & Oikari, A. (2012). Phytotoxicity of low-weight carboxylic acids. Chemosphere, 88(4), 426–431. https://doi.org/10.1016/j.chemosphere.2012.02.058
Jan, B., Brian, M., & Clark, F. C. (2007). Kinetin—A multi-active molecule. International Journal of Biological Macromolecules, 40(3), 182–192. https://doi.org/10.1016/j.ijbiomac.2006.06.024
Li, R., Rosado-Souza, L., Sampathkumar, A., & Fernie, A. R. (2024). The relationship between cell wall and postharvest physiological deterioration of fresh produce. Plant Physiology and Biochemistry, 108568. https://doi.org/10.1016/j.plaphy.2024.108568
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.
Martin-Diana, A. B., Rico, D., Frias, J. M., Barat, J. M., Henehan, G. T. M., & Barry-Ryan, C. (2007). Calcium for extending the shelf life of fresh whole and minimally processed fruits and vegetables: A review. Trends in Food Science and Technology, 18(4), 210–218. https://doi.org/10.1016/j.tifs.2006.11.027
Navarro-Rico, J., Artés-Hernández, F., Gómez, P. A., Núñez-Sánchez, M. Á., Artés, F., & Martínez-Hernández, G. B. (2014). Neutral and acidic electrolysed water kept microbial quality and health-promoting compounds of fresh-cut broccoli throughout shelf life. Innovative Food Science and Emerging Technologies, 21, 74–81. https://doi.org/10.1016/j.ifset.2013.11.004
Ogunniyi, A. D., Tenzin, S., Ferro, S., Venter, H., Pi, H., Amorico, T., & Trott, D. J. (2021). A pH-neutral electrolyzed oxidizing water significantly reduces microbial contamination of fresh spinach leaves. Food Microbiology, 93, 103614. https://doi.org/10.1016/j.fm.2020.103614
Ranjitha, K., Shivashankara, K. S., Rao, D. V. S., Oberoi, H. S., Roy, T. K., & Bharathamma, H. (2017). Improvement in shelf life of minimally processed cilantro leaves through integration of kinetin pretreatment and packaging interventions: Studies on microbial population dynamics, biochemical characteristics and flavour retention. Food Chemistry, 221, 844–854. https://doi.org/10.1016/j.foodchem.2016.11.088
Rattan, S. I. (2021). Nutritional hormetins in ageing and longevity. In Nutrition, food and diet in ageing and longevity (pp. 109–122). Springer. https://doi.org/10.1007/978-3-030-83017-5_6
Salam, S. G., Rashed, M. M., Ibrahim, N. A., Rahim, E. A., Aly, T. A., & Al-Farga, A. (2023). Phytochemical screening and in-vitro biological properties of unprocessed and household processed fenugreek (Trigonella foenum-graecum Linn.) seeds and leaves. Scientific Reports, 13(1), 7032. https://doi.org/10.1038/s41598-023-31888-y
Santos, J., Mendiola, J. A., Oliveira, M. B., Ibáñez, E., & Herrero, M. (2012). Sequential determination of fat- and water-soluble vitamins in green leafy vegetables during storage. Journal of Chromatography A, 1261, 179–188. https://doi.org/10.1016/j.chroma.2012.04.067
Silveira Alexandre, A. C., Ferreira Gomes, B. A., Duarte, G. N., Piva, S. F., Barros Zauza, S., & de Barros Vilas Boas, E. V. (2022). Recent advances in processing and preservation of minimally processed fruits and vegetables: A review—Part 1: Fundamentals and chemical methods. Journal of Food Processing and Preservation, 46(8), e16757. https://doi.org/10.1016/j.focha.2023.100304
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3), 144–158.
Sun, T. P., & Gubler, F. (2004). Molecular mechanism of gibberellin signaling in plants. Annual Review of Plant Biology, 55, 197–223. https://doi.org/10.1146/annurev.arplant.55.031903.141753
Tomás-Callejas, A., Martínez-Hernández, G. B., Artés, F., & Artés-Hernández, F. (2011). Neutral and acidic electrolyzed water as emergent sanitizers for fresh-cut mizuna baby leaves. Postharvest Biology and Technology, 59(3), 298–306. https://doi.org/10.1016/j.postharvbio.2010.09.013
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