First identification of Curvularia xishuangbannaensis Tibpromma &K.D. Hyde, sp. nov as a causal agent of leaf spot in oil palm nurseries andevaluation of fungicidal efficacy

Authors

  • M Amrutha Lakshmi ICAR INDIAN INSTITUTE OF OIL PALM RESEARCH , Indian Institute of Oil Palm Research image/svg+xml Author
  • R Arutselvan 2Regional Centre, ICAR-Central Tuber Crops Research Institute, Bhubaneswar, Odisha, 751019. India , Central Tuber Crops Research Institute image/svg+xml Author
  • M Indraja ICAR IIOPR Pedavegi , Indian Institute of Oil Palm Research image/svg+xml Author
  • G K Challa ICAR IIOPR Pedavegi , Indian Institute of Oil Palm Research image/svg+xml Author
  • K Ramachandrudu ICAR IIOPR Pedavegi , Indian Institute of Oil Palm Research image/svg+xml Author

DOI:

https://doi.org/10.24154/jhs.v20i2.3877

Keywords:

Curvularia, fungicides, leaf spot, management, oil palm

Abstract

Leaf spot caused by Curvularia spp. represents a major constraint in oil palm nurseries, adversely affecting plant health and productivity. This study reports the first identification of Curvularia xishuangbannaensis as the causal agent of oil palm leaf spot, confirmed through colony and spore morphology, ITS-based molecular phylogenetic analysis, and pathogenicity testing using the disc placement method. The in vitro evaluation of fourteen fungicides using the poisoned food technique revealed complete inhibition of mycelial growth by eight fungicides across all tested concentrations, including azoxystrobin 8.3% + mancozeb 66.6% WDG, propiconazole 25% EC, hexaconazole 5% SC, difenconazole 25% EC, triflumizole, tebuconazole 25.9% EC, propiconazole 13.9% + difenconazole 13.9%, and flupyram 17.7% + tebuconazole 11.4% SC. To date, no reports exist on Curvularia species causing leaf spot in oil palm in India or systematic fungicide evaluations against this pathogen. These findings enhance understanding of the pathogenicity of C. xishuangbannaensis and support effective disease management strategies in oil palm nurseries.

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Author Biographies

  • M Amrutha Lakshmi, ICAR INDIAN INSTITUTE OF OIL PALM RESEARCH, Indian Institute of Oil Palm Research

    Scientist, Plant Pathology

  • R Arutselvan, 2Regional Centre, ICAR-Central Tuber Crops Research Institute, Bhubaneswar, Odisha, 751019. India, Central Tuber Crops Research Institute

    Scientist, Plant Pathology, 

    Regional Centre, ICAR-Central Tuber Crops Research Institute, Bhubaneswar, Odisha, 751019. India

  • M Indraja, ICAR IIOPR Pedavegi, Indian Institute of Oil Palm Research

    Young Professional 1, Plant Pathology

  • G K Challa, ICAR IIOPR Pedavegi, Indian Institute of Oil Palm Research

    Senior Research Fellow, Crop Protection

  • K Ramachandrudu, ICAR IIOPR Pedavegi, Indian Institute of Oil Palm Research

    Principal Scientist, Crop Production

References

Agustina, D., Prihatna, C., & Suwanto, A. (2019). Rapid inoculation technique and biological control of leaf spot disease in oil palm. International Journal of Oil Palm, 2(1), 1–11. https://doi.org/10.35876/ijop.v2i1.24

Anuar, M. A. S. S., & Ali, N. S. (2022). Significant oil palm diseases impeding global industry. Sains Malaysiana, 51, 707–721. https://doi.org/10.17576/jsm-2022-5103-06

Azlan, A. M. N., Ahmad, Z. A. M., Idris, A. S., & Vadamalai, G. (2018). Assessment of leaf spot and anthracnose diseases in nurseries and its relationship with oil palm seedling ages. International Journal of Advanced Multidisciplinary Research, 5(1), 19–26. https://doi.org/10.22192/ijamr.2020.07.04.004

Bisht, S., Balodi, R., Ghatak, A., & Kumar, P. (2016). Determination of susceptible growth stage and efficacy of fungicidal management of Curvularia leaf spot of maize caused by Curvularia lunata. Maydica, 61, M27.

Brahima, C., Klotioloma, C., Tchima, D., Seydou, T., & Daouda, K. (2023). Characterization of fungal diseases in palm oil nurseries and implementation of control methods. American Journal of Bioscience and Bioengineering, 11(6), 92–102. https://doi.org/10.11648/j.bio.20231106.11

Brown, W. (1924). A method of isolating single strains of fungi by cutting out a hyphal tip. Annals of Botany, 38(150), 402–404. https://doi.org/10.1007/BF02860105

Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791. https://doi.org/10.2307/2408678

Garcia-Aroca, T., Doyle, V., Singh, R., Price, T., & Collins, K. (2018). First report of Curvularia leaf spot of corn, caused by Curvularia lunata, in the United States. Plant Health Progress, 19(2), 140–142. https://doi.org/10.1094/PHP-02-18-0008-BR

Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes: Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

Hewitt, H. G. (1998). Fungicides in plant protection. CAB International.

Ismail, S. I., Zulperi, D., Norddin, S., & Ahmad-Hamdani, S. (2017). First report of Neopestalotiopsis saprophytica causing leaf spot of oil palm (Elaeis guineensis) in Malaysia. Plant Disease, 101(10), 1821. https://doi.org/10.1094/PDIS-02-17-0271-PDN

Koli, P., Bhardwaj, N. R., & Mahawer, S. K. (2019). Agrochemicals: Harmful and beneficial effects in climate changing scenarios. In Climate change and agricultural ecosystems (pp. 65–94). Woodhead Publishing. https://doi.org/10.1016/B978-0-12-816483-9.00004-9

Kovachich, W. G. (1954). Cercospora elaeidis leaf spot of the oil palm. Transactions of the British Mycological Society, 37(3), 209–211. https://doi.org/10.1016/S0007-1536(54)80002-7

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096

Lakshmi, M. A., Indraja, M., Challa, G. K., Arutselvan, R., & Suresh, K. (2024). Evaluation of new generation fungicides against Ganoderma-induced basal stem rot of oil palm. Journal of Mycology and Plant Pathology, 54(2), 101–110. https://doi.org/10.59467/JMPP.2024.54.101

Lekete, E., Osekre, E. A., & Andoh-Mensah, E. (2019). Outbreak and in vitro management of leaf spot disease caused by Pestalotiopsis sp. on oil palm seedlings in Ghana. Malaysian Journal of Microbiology, 15, 419–424. https://doi.org/10.21161/mjm.180170

Martínez, L., & Plata-Rueda, A. (2013). Lepidoptera vectors of Pestalotiopsis fungal disease in oil palm plantations from Colombia. International Journal of Tropical Insect Science, 33(4), 239–246. https://doi.org/10.1017/S1742758413000283

Nasehi, A., Sathyapriya, H., & Wong, M. Y. (2020). First report of leaf spot on oil palm caused by Phyllosticta capitalensis in Malaysia. Plant Disease, 104(1), 288. https://doi.org/10.1094/PDIS-07-19-1505-PDN

Poole, N. F., & Arnaudin, M. E. (2014). The role of fungicides for effective disease management in cereal crops. Canadian Journal of Plant Pathology, 36(1), 1–11. https://doi.org/10.1080/07060661.2014.905226

Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454

Tamura, K., Nei, M., & Kumar, S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences, 101, 11030–11035. https://doi.org/10.1073/pnas.0404206101

Vijay, V., Pimm, S. L., Jenkins, C. N., & Smith, S. J. (2016). The impacts of oil palm on recent deforestation and biodiversity loss. PLOS ONE, 11(7), e0159668. https://doi.org/10.1371/journal.pone.0159668

White, T. J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis et al. (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). Academic Press.

Zheng, L., Xi, P., SiTu, J. J., Chen, X. N., Li, J., Qin, X. D., Shen, H. F., & Xie, C. P. (2017). First report of Phoma herbarum causing leaf spot of oil palm (Elaeis guineensis) in China. Plant Disease, 101(4), 629–630. https://doi.org/10.1094/PDIS-09-16-1343-PDN

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Published

31-12-2025

Data Availability Statement

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Section

Research Papers

How to Cite

Amrutha Lakshmi, M., Arutselvan, R., Indraja, M., Challa, G. K., & Ramachandrudu, K. (2025). First identification of Curvularia xishuangbannaensis Tibpromma &K.D. Hyde, sp. nov as a causal agent of leaf spot in oil palm nurseries andevaluation of fungicidal efficacy. Journal of Horticultural Sciences, 20(2). https://doi.org/10.24154/jhs.v20i2.3877

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