First identification of Curvularia xishuangbannaensis Tibpromma &K.D. Hyde, sp. nov as a causal agent of leaf spot in oil palm nurseries andevaluation of fungicidal efficacy
DOI:
https://doi.org/10.24154/jhs.v20i2.3877Keywords:
Curvularia, fungicides, leaf spot, management, oil palmAbstract
Leaf spot caused by Curvularia spp. represents a major constraint in oil palm nurseries, adversely affecting plant health and productivity. This study reports the first identification of Curvularia xishuangbannaensis as the causal agent of oil palm leaf spot, confirmed through colony and spore morphology, ITS-based molecular phylogenetic analysis, and pathogenicity testing using the disc placement method. The in vitro evaluation of fourteen fungicides using the poisoned food technique revealed complete inhibition of mycelial growth by eight fungicides across all tested concentrations, including azoxystrobin 8.3% + mancozeb 66.6% WDG, propiconazole 25% EC, hexaconazole 5% SC, difenconazole 25% EC, triflumizole, tebuconazole 25.9% EC, propiconazole 13.9% + difenconazole 13.9%, and flupyram 17.7% + tebuconazole 11.4% SC. To date, no reports exist on Curvularia species causing leaf spot in oil palm in India or systematic fungicide evaluations against this pathogen. These findings enhance understanding of the pathogenicity of C. xishuangbannaensis and support effective disease management strategies in oil palm nurseries.
Downloads
References
Agustina, D., Prihatna, C., & Suwanto, A. (2019). Rapid inoculation technique and biological control of leaf spot disease in oil palm. International Journal of Oil Palm, 2(1), 1–11. https://doi.org/10.35876/ijop.v2i1.24
Anuar, M. A. S. S., & Ali, N. S. (2022). Significant oil palm diseases impeding global industry. Sains Malaysiana, 51, 707–721. https://doi.org/10.17576/jsm-2022-5103-06
Azlan, A. M. N., Ahmad, Z. A. M., Idris, A. S., & Vadamalai, G. (2018). Assessment of leaf spot and anthracnose diseases in nurseries and its relationship with oil palm seedling ages. International Journal of Advanced Multidisciplinary Research, 5(1), 19–26. https://doi.org/10.22192/ijamr.2020.07.04.004
Bisht, S., Balodi, R., Ghatak, A., & Kumar, P. (2016). Determination of susceptible growth stage and efficacy of fungicidal management of Curvularia leaf spot of maize caused by Curvularia lunata. Maydica, 61, M27.
Brahima, C., Klotioloma, C., Tchima, D., Seydou, T., & Daouda, K. (2023). Characterization of fungal diseases in palm oil nurseries and implementation of control methods. American Journal of Bioscience and Bioengineering, 11(6), 92–102. https://doi.org/10.11648/j.bio.20231106.11
Brown, W. (1924). A method of isolating single strains of fungi by cutting out a hyphal tip. Annals of Botany, 38(150), 402–404. https://doi.org/10.1007/BF02860105
Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791. https://doi.org/10.2307/2408678
Garcia-Aroca, T., Doyle, V., Singh, R., Price, T., & Collins, K. (2018). First report of Curvularia leaf spot of corn, caused by Curvularia lunata, in the United States. Plant Health Progress, 19(2), 140–142. https://doi.org/10.1094/PHP-02-18-0008-BR
Gardes, M., & Bruns, T. D. (1993). ITS primers with enhanced specificity for basidiomycetes: Application to the identification of mycorrhizae and rusts. Molecular Ecology, 2, 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
Hewitt, H. G. (1998). Fungicides in plant protection. CAB International.
Ismail, S. I., Zulperi, D., Norddin, S., & Ahmad-Hamdani, S. (2017). First report of Neopestalotiopsis saprophytica causing leaf spot of oil palm (Elaeis guineensis) in Malaysia. Plant Disease, 101(10), 1821. https://doi.org/10.1094/PDIS-02-17-0271-PDN
Koli, P., Bhardwaj, N. R., & Mahawer, S. K. (2019). Agrochemicals: Harmful and beneficial effects in climate changing scenarios. In Climate change and agricultural ecosystems (pp. 65–94). Woodhead Publishing. https://doi.org/10.1016/B978-0-12-816483-9.00004-9
Kovachich, W. G. (1954). Cercospora elaeidis leaf spot of the oil palm. Transactions of the British Mycological Society, 37(3), 209–211. https://doi.org/10.1016/S0007-1536(54)80002-7
Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547–1549. https://doi.org/10.1093/molbev/msy096
Lakshmi, M. A., Indraja, M., Challa, G. K., Arutselvan, R., & Suresh, K. (2024). Evaluation of new generation fungicides against Ganoderma-induced basal stem rot of oil palm. Journal of Mycology and Plant Pathology, 54(2), 101–110. https://doi.org/10.59467/JMPP.2024.54.101
Lekete, E., Osekre, E. A., & Andoh-Mensah, E. (2019). Outbreak and in vitro management of leaf spot disease caused by Pestalotiopsis sp. on oil palm seedlings in Ghana. Malaysian Journal of Microbiology, 15, 419–424. https://doi.org/10.21161/mjm.180170
Martínez, L., & Plata-Rueda, A. (2013). Lepidoptera vectors of Pestalotiopsis fungal disease in oil palm plantations from Colombia. International Journal of Tropical Insect Science, 33(4), 239–246. https://doi.org/10.1017/S1742758413000283
Nasehi, A., Sathyapriya, H., & Wong, M. Y. (2020). First report of leaf spot on oil palm caused by Phyllosticta capitalensis in Malaysia. Plant Disease, 104(1), 288. https://doi.org/10.1094/PDIS-07-19-1505-PDN
Poole, N. F., & Arnaudin, M. E. (2014). The role of fungicides for effective disease management in cereal crops. Canadian Journal of Plant Pathology, 36(1), 1–11. https://doi.org/10.1080/07060661.2014.905226
Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406–425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Tamura, K., Nei, M., & Kumar, S. (2004). Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences, 101, 11030–11035. https://doi.org/10.1073/pnas.0404206101
Vijay, V., Pimm, S. L., Jenkins, C. N., & Smith, S. J. (2016). The impacts of oil palm on recent deforestation and biodiversity loss. PLOS ONE, 11(7), e0159668. https://doi.org/10.1371/journal.pone.0159668
White, T. J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis et al. (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). Academic Press.
Zheng, L., Xi, P., SiTu, J. J., Chen, X. N., Li, J., Qin, X. D., Shen, H. F., & Xie, C. P. (2017). First report of Phoma herbarum causing leaf spot of oil palm (Elaeis guineensis) in China. Plant Disease, 101(4), 629–630. https://doi.org/10.1094/PDIS-09-16-1343-PDN
Downloads
Published
Data Availability Statement
None
Issue
Section
License
Copyright (c) 2025 M Amrutha Lakshmi, R Arutselvan, M Indraja, G K Challa, K Ramachandrudu (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.



. 








