Metabolite profiling in Mango (Mangifera indica L.) pollen grains in relation to viability
DOI:
https://doi.org/10.24154/jhs.v14i1.710Keywords:
Pollen,, viability,, LC-MS,, amino acids,, sugars,, mango,, hormonesAbstract
Mango productivity is affected mainly by irregular flowering, proportion of bisexual flowers, poor pollination and fertilization and fruit drop. Poor fruit set in some of the varieties may be associated with the lower pollen viability. The present experiment was initiated to assess the viability of pollen grains and their metabolites in three mango cultivars Amrapali, Alphonso and Totapuri which are differing in their fruit set intensity. The profiling of sugars, amino acids and some of the phytohormones were analysed using Liquid Chromatography-Mass Spectrometry (LC-MS/MS). Assessment of pollen grains in three mango cultivars indicated that free sugars such as fructose and glucose, and available amino acids including serine, proline, lysine, phenylalanine, alanine and glutamic acid were predominantly higher in all the cultivars. Phytohormones like IAA, IBA, ABA, GA,
zeatin, jasmonic acid and salicylic acid were significantly different in low fruit setting cultivars Alphonso and Totapuri compared to high fruit setting cultivar Amrapali. In cv. Alphonso all the metabolites were higher at anthesis but later decreased drastically compared to cvs. Totapuri and Amrapali. Pollen viability percentage was significantly higher in cv. Amrapali than in cvs. Totapuri, Alphonso. Among all the cultivars, Amrapali maintained better chemical composition at anthesis and also at two hours after anthesis compared to cvs. Totapuri and Alphonso.
References
Abourayya, M.S., Kassim, N.E., El-Sheikh, M.H. and Rakha, A.M. 2011. Comparative study between inflorescence characteristics, pollen viability, germination and dimensions of Tommy Atkins, Kent and Keitt mango cultivars. Life
Sci. J. 8 (1):100–105.
Abd El-Hadi, S.M.K. 2006. Evaluation studies on some mango varieties. Fac. Agric., Al-Azhar Univ., Egypt, pp. 166 (M.Sc. Thesis).
Aloni, B., Peet, M.M., Pharr, M. and Karni, L. 2001. The effect of high temperature and high atmospheric CO2 on carbohydrate changes in bell pepper (Capsicum annuum) pollen in relation to its germination. Physiol. Plant. 112:505–512.
Aloni, R., Aloni, E., Langhans, M. and Ullrich, C.I. 2006. Role of auxin in regulating Arabidopsis flower development. Planta 223:315–328.
Basuny, A.M., Arafat, S.M. and Soliman, H.M. 2013. Chemical analysis of olive and palm pollen: antioxidant and antimicrobial activation properties. Wudpecker J. Food Tech. 1:14–21.
Cecchetti, V., Altamura, M.M., Brunetti, P., Petrocelli, V., Falasca, G., Ljung, K., Costantino, P. and Cardarelli, M. 2013. Auxin controls Arabidopsis anther dehiscence by regulating endothecium lignification and jasmonic acid biosynthesis. Plant J. 74:411–422.
Cheng, Y., Dai, X. and Zhao, Y. 2006. Auxin biosynthesis by the yucca flavin monooxygenases controls the formation of
floral organs and vascular tissues in Arabidopsis. Genes Dev. 20:1790–1799.
Chhun, T., Aya, K., Asano, K., Yamamoto, E., Morinaka, Y., Watanabe, M., Kitano, H., Ashikari, M., Matsuoka, M. and Ueguchi- Tanaka, M. 2007. Gibberellin regulates pollen viability and pollen tube growth in rice. Plant Cell 19:3876–3888.
Dahshan, D.I.M. 1971. Studies concerning growth and fruiting in some mango varieties. Fac. Agric. Ain Shams Univ., Egypt, pp.171 (M.Sc. Thesis).
Desai, A.G., Limaye, V.P. and Gunjate, R.T. 1985. Floral biology of Alphonso, Goamankur and Kesar varieties of mango. J Maharashtra Agric. Univ, 10(2):193-195.
da Silva, G.R., da Natividade, T.B., Camara, C.A., da Silva, E.M.S., de Assis Ribeiro dos Santos, F. and Silva, T.M.S. 2014. Identification of sugar, amino acids and minerals from the pollen of Jandaíra stingless bees (Melipona subnitida). Food Nutri. Sci. 5(11):1015-1021.
Davenport, T.L. 2009. Reproductive physiology. In: Litz, R.E. (Ed.), The Mango: Botany Production and Uses, 2nd edition. CAB International, Wallingford, UK, pp. 97–169.
El-Kady, M.I.E. 1973. Physiological studies on flowering and fruit set in some mango varieties especially in Taimour. Fac. Agric., Cairo Univ., Egypt, pp. 195 (M.Sc.Thesis).
El-Masry, S.M. 2001. Selection of some mango seedlings grown in Assiut. Fac. Agric., Assiut Univ., Egypt, pp. 195 (Ph.D. Thesis).
Geetha, G.A., Shivashankara, K.S. and Roy, T.K. 2016. Floral biology and stigma biochemistry of mango cultivars with contrasting fruit set. Indian J. Plant Physiol. 21(4):537-544.
Gillaspy, G., Ben-David, H. and Gruissem, W. 1993. Fruits: a developmental perspective. The Plant Cell 5:1439–1451. Missing in text?
Goto, N. and Pharis, R.P. 1999. Role of gibberellins in the development of floral organs of the gibberellin-deficient mutant, ga1-1, of Arabidopsis thaliana. Can. J. Bot. 77:944–954.
Hassan, M.M. 2011. Chemical Composition and Nutritional Value of Palm Pollen Grains. Global J. Biotechnol. Biochem. 6(1):1-7.
Herrero, M. 2003. Male and female synchrony and the regulation of mating in flowering plants. Philosop. Trans. Royal Soc. London Ser. B Bio. Sci. 358:1019-1024.
Issarakraisila, M. and Considine, J.A. 1994. Effect of temperature on pollen viability in Mango cv. Kensington. Ann. Bot. 73(3):231-240.
Issarakraisila, M., Considine, J.A. and Turner, D.W. 1992. Seasonal effects on floral biology and fruit set of mangoes in a warm temperature region of Western Australia. Acta Horti. 321:626-635.
Kedzia, B. and Holderna-Kedzia, E. 2012. New studies on biological properties of pollen. PostępyFitoterapii, 1:48–54.
Koul, A.K. and Paliwal, R.L. 1961. Inorganic acid test for pollen viability. Agra univer. J. Res. Sci. 10:85-90.
Kovaleva, L. and Zakharova, E. 2003. Hormonal status of the pollen–pistil system at the progamic phase of fertilization after compatible and incompatible pollination in petunia hybrida L. Sex Plant Reprod. 16:191–196.
Kovaleva, L.V. and Zakharova, E.V. 2004. Gametophyte– sporophyte interactions in the pollen–pistil system: 4. The hormonal status and the mechanism of self-incompatibility. Russ. J. Plant Physiol., Engl. Transl. pp.402–406.
Leah Karni and Beny Aloni. 2002. Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): possible role in pollen germination under conditions of high temperature and co2 enrichment. Ann Bot. 90(5):607–612.
Mallik, P.C. 1957. Morphology and biology of the mango flower. Indian J. Hort. 4:1-23.
Mattioli, R., Biancucci, M., Lonoce, C., Costantino, P. and Trovato, M. 2012. Proline is required for male gametophyte development in Arabidopsis. BMC Plant Biol. 12(1):236.
Mol, R., Filek, M., Machackova, I. and Matthys- Rochon, E. 2004. Ethylene synthesis and auxin augmentation in pistil tissues are important for egg cell differentiation after pollination in maize. Plant Cell Physiol. 45:1396–1405.
Mukherjee, S.K. 1949. The mango and its relatives. Sci. cult. 15:5-9.
Mutters, R.G., Ferreira, L.G.R. and Hall, A.E. 1989. Proline content of the anthers and pollen of heat tolerant and heat-sensitive cowpea subjected to different temperatures. Crop Sci. 29:1497– 1500.
Nishiyama, I. 1984. Climatic influence on pollen formation and fertilisation. In: Tsunoda, S., Takahashi, N., eds. Biology of rie. Tokyo; Japan scientific societies press, 153-171.
Owens, J.N., Simpson, S.J. and Molder, M. 1980. The pollination mechanism in yellow cypress (Chamaecyparis nootkatensis). Can. J. Forest Res. 10:564-572.
Parish, R.W., Phan, H.A., Iacuone, S. and Li, S.F. 2013. Tapetal development and abiotic stress: A centre of vulnerability. Funct. Plant Biol. 39:553–559.
Pimentel, R.B., Coronel, R.E. and Espino, R.F.C. 1984. Floral biology and fruit set in mango (Mangifera indica L.) cultivars: ‘Carabao’, ‘Pico’ and ‘KanchaMitha’. Philippine J. Crop Sci. 9:47–51.
Randhawa, G.S. and Damodaran, V.K. 1961. Studies on floral biology and sex ratio in mango (Mangifera indica L.) I. A review. Indian J. Horti. 18:9-35.
Roulston, T.H. and Cane, J.H. 2000. Pollen nutritional content and digestibility for animals. Plant Sys. Evol. 222(1-4):187–209.
Sen, P.K., Mallik, P.C. and Ganguly, B.D. 1946. Hybridization of the mango. Indian J. Hort, 4(4).
Singh, R.N. 1954. Studies in floral biology and subsequent development of fruits in the Mango (Mangifera indica L.) dasheri and langra. Indian J. Horti. 11(3):69-88.
Singh, S.N. 1963. Studies on the morphology, viability and preservation of pollen grains of mango (Mangifera indica L.), litchi (Litchi chinensis Sonn.) and loquat (Eriobothrya japonica Lind!). Agra Univer. J. Res. Sci. 12:317-322.
Singh, S.N. and Singh, A.P. 1961. Studies on the morphology and viability of the pollen grains of mango. Hort. Adv. 5:121-144.
Spencer, J.L. and Kennard, W.C. 1955. Studies on Mango (Mangifera indica L.) fruit set in Puerto Rico. Tropical Agri.Trin. 32:323–330.
Stanley, R.G. and Linskens, H.F. 1974. Pollen: biology, biochemistry, management. 1st edn. Springer, Heidelberg, Germany.
Stosser, R., Hartman, W. and Anvari, S.F. 1996. General aspects of pollination and fertilization of pome and stone fruits. Acta Horti. 423:15-21.
Szczesna, T. 2006. Long chain fatty acids composition of honeybee collected pollen. J. Apicult. Sci. 50:65-79.
Tsang, M.J. and Chang, W.N. 1983. Effects of temperature and growth regulators on flowering and fruit set of mango. J. Chin. Soc. Horti. Sci. 29:87-95.
Wu, J., Qin, Y. and Zhao, J. 2008. Pollen tube growth is affected by exogenous hormones and correlated with hormone changes in styles in Torenia fournieri L. Plant growth regul. 55(2):137-148.
Young, T.W. 1955. Influence of temperature on growth of mango pollen. Pro. Florida State Horti. Soc. 68:308–313.
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