Optimization of GA3 concentration for improved bunch and berry quality in grape cv. Crimson Seedless (Vitis vinifera L)
GA3 concentration for improving quality of Crimson Seedless grapes
DOI:
https://doi.org/10.24154/jhs.v16i2.1072Keywords:
Grapes, Crimson seedless, Cluser compactness, Rachis elongation, Fruit quality, GA3Abstract
Crimson Seedless is a coloured seedless grape, gaining popularity in India for its attractive colour, bunch and berry quality with better shelf life. In cultivation of any seedless grape variety, application of GA3 at different stages is very much essential to produce good quality berries and bunches. However, this variety is highly sensitive to excess application of GA3 and practicing GA3 schedule similar to that of Thompson Seedless grapes adversely affects bunch quality. Hence, there was a need to standardize mild dose of GA3 just for rachis elongation which will help to reduce bunch compactness to a greater extent. Hence, an experiment was initiated to standardize concentration of GA3 for rachis elongation of Crimson Seedless grapes. Three different concentrations of GA3 {viz., 5 ppm (T1), 7.5 ppm (T2) and 10 ppm (T3)} were sprayed during pre bloom stage and compared with unsprayed control (T4). Among different treatments, pre-bloom spray of GA3@5 ppm could produce less compact bunches with highest average bunch weight, berry weight, berry length and TSS. However, bunches sprayed with 7.5 ppm and 10 ppm GA3 could also produce good quality bunches average berry weight and TSS was less. Because of severe coiling of rachis at 7.5 ppm and 10 ppm GA3 spraying, bunches were too straggly compared to spraying of 5 ppm GA3. The control bunches without GA3 spray produced very compact clusters with less average bunch weight, berry weight, berry diameter and berry length.
References
Acheampong, A.K., Hu, J., Rotman, A., Zheng, C., Halaly, T. AndTakebayashi, Y. 2015. Functional characterization and developmental expression profiling of gibberellin signalling components in Vitis vinifera. J. Expt. Bot., 66: 1463–1476.
Aguero, C.,Vigliocco, A., Abdala, G. and Tizio, R. 2000. Effect of gibberellic acid and uniconazol on embryo abortion in the stenospermocarpic grape cultivars Emperatriz and Perlon. Plant Growth Regulation, 30:9–16.
Avenant, J. H., 2017. Effect of Gibberellic acid (GA3) and N (2-Chloro-4 pyridyl), - N-phenylurea (CPPU) treatments to reduce or eliminate browning of white table grape cultivars. Acta Horticulture, 4: 189-193.
Brown, D., Wrightman, R., Zhang, Z., Gomez, L.D., Atanassov, I., Bukowski, J.P., Tryfona, T., McQueen-Mason, S.J., Dupree, P. and Turner, S. 2011. Arabidopsis genes IRREGULAR XYLEM (IRX15) and IRX15L encode DUF579-containing proteins that are essential for normal xylan deposition in the secondary cell wall. Plant Journal, 3: 401-413.
Chadha, K.L. and Shikhamany, S. D.1999. The Grape-Improvement, Production and Post Harvest Management (ISBN: 81-85048-40-1), Malhotra Publishing House, New Delhi. Pp: 689
Cheng, C., Jiao, C., Singer, S.D., Gao, M., Xu, X., Zhi, Y.Z., Fei, Z., Wang, Y. andWang, X. 2015.Gibberellin-induced changes in the transcriptome of grapevine (Vitis labrusca × V. vinifera) cv. Kyoho flowers. BMC Genomics, 16: 128.
Cheng, C., Xu,X,, Singer, S.D., Li,J., Zhang, H. and Gao, M.2013. Effect of GA3 treatment on seed development and seed-related gene expression in grape. PLoS ONE, 8:e80044.
Dokoozlian, N.K. and Peacock, W.L. 2001. Gibberellic acid applied at bloom reduces fruit set and improves size of ‘Crimson Seedless’ table grapes. Hort Sci. 36: 706-709.
Domingos, S.,Fino, J., Cardoso, V., Sanchez, C., Ramalho, J.C.,Larcher, R., Paulo, O.S., Oliveira C.M. and Goulao, L.F. 2016. Shared and divergent pathways for flower abscission are triggered by gibberellic acid and carbon starvation in seedless Vitis vinifera L. BMC Plant Biol.16: 38. https ://doi.org/10.1186/s1287 0-016-0722-7
Espinoza, C., Medina, C., Somerville. S. and Arce-Johnson. P.2007. Senescence-associated genes induced during compatible viral interactions with grapevine and Arabidopsis. J. Expt.Bot. 58:3197–3212.
Fraser, W.J. 2007. Manipulation of the taste of Regal Seedless (Vitis Vinifera L.) table grapes. Master of Agriculture (Viticulture and Oenology) thesis, submitted to University of Stellenbosch. Pp: 173.
Fuleki, T. 1969. The anthocyanins of strawberry, rhubarb, radish and onion. Food Sci. 34: 365-369.
Ghule, S.M., Upadhyay, A. Jogaiah, S.2019. Proteomic analysis of GA3 induced berry elongation in grape (Vitis vinifera L) cv. Thompson Seedless. Biosci. Biotech. Res. Asia. 16: 85-92.
Ghule, S.M., Upadhyay, A., Jogaiah, S., Patil,S.S., Kadoo, N.Y. and Gupta, V.S. 2019.Whole proteome analysis of GA3 response at panicle stage in grape (Vitis vinifera L) cv. Thompson Seedless. J.Plant Growth Regulation.https://doi.org/10.1007/s00344-019-10041-y
Guerios, I.T., Chiarotti, F., Cuquel, F.L. and Biasi, L.A. 2016. Growth regulator improves bunch and berry character in ‘Niagara Rosada’ grape. Acta Hort., The Hauge, 1115: 243-248.
Harrell,D.C. and Williams, L.E. 1987. Influence of girdling and gibberellic acid application at fruit set on Ruby Seedless and Thompson Seedless grapes. Am. J. Enol.Vitic. 38: 83-88.
Iqbal, N., Nazar, R., Iqbal, M., Khan, R., Masood, A. and Khan, A.N. 2011. Role of gibberellins in regulation of source sink relations under optimal and limiting environmental conditions. Current Sci. 100: 998-1007.
Liszkay, A., van der Zalm, E. and Schopfer, P. 2004. Production of reactive oxygen intermediates (O2, H2O2, and OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol. 136: 3114-3123.
Looney, N.E. and Wood, D.F. 1977. Some cluster thinning and gibberellic effect on fruit set, berry size, vine growth and yield of De Chaunac grapes. Canadian J. Plant Sci. 57: 653-659.
Molitor,D., Behr M.,Hoffmann,L. And Evers, D. 2012.Impact of grape cluster division on cluster morphology and bunch rot epidemic. Am. J.Enol.Vitic. 63: 508-514.
Murcia, G., Pontin, M., Reinoso, H., Baraldi, R., Bertazza, G., Gomez- Talquenca, S., Bottini, R. and Piccoli, P.N. 2016. ABA and GA3 Increase carbon allocation in different organs of grapevine plants by inducing accumulation of non-structural carbohydrates in leaves enhancement of phloem area and expression of sugar tr ansporters. Physiologia Plantarum,156: 323–337. https ://doi.org/10.1111/ppl.12390
Nunan, K.J., Davies, C., Robinson, S.P. and Fincher, G.B. 2001. Expression patterns of cell wallmodifying enzymes during grape berry development. Planta, 214: 257–264. https ://doi.org/10.1007/s0042 50100609
Ozer, C., Yasasin, A.S., Ergonul, O. and Aydin, S. 2012. The effects of berry thinning and gibberellin on ‘RecelUzumu’ table grapes. Pakistan J. Agri. Sci. 49: 105-112.
Peppi, M.C., Fidelibus, M.W. and Dokoozlian, N. 2006. Abscisic acid application timing and concentration affect firmness, pigmentation and colour of ‘Flame Seedless’ grapes. Hort Sci.41: 1440–1445.
Pérez, F.J. and Gómez, M. 2000. Possible role of soluble invertase in the gibberellic acid berrysizing effect in Sultana grape. Plant Growth Reg. 30:111–116.
Rusjan, D.2010. Impact of gibberellin (GA3) on sensorial quality and storability of table grape (Vitis vinifera L) Acta Agricultura Slovenica, 95:163-173.
Schopfer,P. 2001. Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth. Plant J.28: 679–688. https ://doi.org/10.1046/j.1365-313x.2001.01187.x
Singh, N.S. and Khanduja, S.D. 1977. Physical and biochemical changes during maturation of grapes (Vitis vinifera), Indian J. Hort. 34: 354
Singh, S., Singh, I.S. and Singh, D.N. 1993.Physicochemical changes during development of seedless grapes (Vitis vinifera L) Orissa J. Hort. 21: 43-46
Singleton, V.L. and Rossi, J.A. 1965. A colorimetry method of total phenolics with phosphomolybdic- phosphotungstanic acid reagents. Am. J. Enol.Vitic.16: 144-158.
Somyog, M. 1952. Notes on sugar determination. J. Biol. Chem. 195: 19.
Upadhyay, A., Maske S., Jogaiah, S., Kadoo, N.Y. and Gupta, V.S. 2018. GA3 application in grapes (Vitis vinifera L.) modulates different sets of genes at cluster emergence, full bloom, and berry stage as revealed by RNA sequence-based transcriptome analysis. Func. Int. Genomics. 18: 439-455.
Wang, X., Zhao, M., Wu,W., Korir., N.K., Qian,Y. and Wang, Z. 2017. Comparative transcriptome analysis of berry-sizing effects of gibberellin (GA3) on seedless Vitis vinifera L. Genes & Genomics, 39: 493-507.
Wang, Z., Zhao, F., Zhao, X., Ge, H., Chai, L., Chen, S., Perl, A. and Ma, H. 2012. Proteomic analysis of berry sizing effect of GA3 on seedless Vitis vinifera L. Proteomics, 12: 86-94.
Weaver, R.J. 1975. Effect of time of application of potassium gibberlate on cluster development of Zinfandel grapes. Vitis, 14: 97-102.
Weaver, R.J.1958. Effect of gibberellic acid on fruit set and berry enlargement in seedless grapes of Vitis vinifera. Nature, 181:851-852.
Weaver, R.J. and McCune, S.B. 1960. Further studies with gibberellin on Vitis vinifera grapes. Bot. Gazette, 121: 155-162.
Weaver, R.J. and Pool, R.M.1965. Bloom spraying with gibberellin loosens clusters of Thompson Seedless grapes. California Agri. 19: 14-15.
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