Genetic analysis and identification of SSR marker linked topowdery mildew resistance in bitter gourd (Momordica charantia L.)
DOI:
https://doi.org/10.24154/jhs.v19i1.2264Keywords:
Bitter gourd, inheritance, powdery mildew, resistance, SSR markerAbstract
The inheritance of powdery mildew resistance in bitter gourd resistant line IIHR-144-1 was studied in cross with two susceptible lines Arka Harit and IIHR Sel-5-8. The segregation ratios in F2 and backcross progenies indicated that resistance is governed by dominant-recessive epistasis or dominant inhibitory epistasis, indicating the involvement of more than one pairs of genes, one dominant and one recessive for resistance along with other background minor genes in the resistant line IIHR-144-1. Bulk segregant analysis of F2 population of cross between IIHR-144-1 and Arka Harit using SSR markers identified McSSR 57 produced a polymorphic DNA fragment that co-segregated with disease reaction. Considering the cross compatibility between Momordica charantia L. and M. charantia var. muricata (IIHR-144-1) the resistance trait can be transferred to cultivated species through hybridization and selection.
References
Behera, T. K., Gaikwad, A. B., Singh, A. K., & Staub, J. E. (2008a). Comparative analysis of genetic diversity in Indian bitter gourd (Momordica charantia L.) using RAPD and ISSR markers for developing crop improvement strategies.
Scientia Horticulturae, 115, 209-217. https://doi.org/10.1016/j.scienta.2007.08.013
Behera, T. K., Gaikwad, A. B., Singh, A. K., & Staub. J. E. (2008b). Relative efficiency of DNA markers (RAPD, ISSR and AFLP) in detecting genetic diversity of bitter gourd (Momordica charantia L.). Journal of the Science of Food
and Agriculture, 88, 733-737. https://api.semanticscholar.org/CorpusID:27588497
Campbell, C. L., & Madden, L. V. (1990). Introduction to plant epidemiology. Wiley. p531.
Dey, S. S., Singh, A. K., Chandel, D., & Behera. T. K. (2006). Genetic diversity of bitter gourd (Momordica charantia L.) genotypes revealed by RAPD markers and agronomic traits. Scientia Horticulturae, 109, 21-28. https://doi.org/10.1016/j.scienta.2006.03.006
Dhillon, N. P. S., Sanguansil, S., Srimat, S., Cheng, H., Lin, C., Srinivasan, R., Kenyon, L., Schafleitner, R., Yang, R., & Hanson, P. (2016). Status of cucurbit breeding at AVRDC -The world vegetable center. In: Kozik E. U., Paris H. S. (Eds.). The XIth Eucarpia meeting on cucurbit genetics and breeding 2016, Warsaw, Poland, 21-25.
Dhillon, N. P. S., Sanguansil, S., Srimat, S., & Laenoi, S. (2019). Inheritance of resistance to cucurbit powdery mildew in bitter gourd. Hortscience, 54(6), 1013–1016. https://doi.org/10.21273/HORTSCI13906-19
Doyle, J. J., & Doyle, J. J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19, 11-15.
Fanourakis, N. E. (1990). Screening procedure for powdery mildew resistance in cucumber. Acta Horticulture, 287, 147-154. https://doi.org/10.17660/ActaHortic.1991.287.14
Giovanni, C. D., Dell’Orco, P., Bruno, A., Ciccarese, F., Lotti, C., & Ricciardi, L. (2004). Identification of PCR-based marker (RAPD, AFLP) linked to a novel powdery mildew resistance gene (ol-2) in tomato. Plant Science, 166, 41-48. https://doi.org/10.1016/j.plantsci.2003.07.005
Guo, D. L., Zhang, J. P., Xue, Y. M., & Hou, X. G. (2012). Isolation and characterization of 10 SSR markers of Momordica charantia (Cucurbitaceae). American Journal of Botany, e182-e183. doi: 10.3732/ajb.1100277
Huang, X., & Roder, M. (2004). Molecular mapping of powdery mildew resistance genes in wheat: A review. Euphytica, 137, 203-223. https://d o i . o r g / 1 0 . 1 0 2 3 B : E U P H .0000041576.74566.d7
Jenkins, S. F., & Wehner, T. C. (1983). A system for the measurement of foliar diseases of cucumber. Cucurbit Genetic Cooperative Report, 6, 10-12.
Ji, Y., Luo, Y., Hou, B., Wang, W., Zhao, J., Yang, L., Xue, Q., & Ding, X. (2012). Development of polymorphic microsatellite loci in Momordica charantia (Cucurbitaceae) and their transferability to other cucurbit species. Scientia
Horticulturae, 140, 115-118. https://doi.org/10.1016/j.scienta.2012.03.024
Lisbona, F. J. Y., Sese, A. I. L., & Guillamon, M. L. G. (2010). Inheritance of resistance to races 1, 2 and 5 of powdery mildew in the melon TGR 1551. Plant Breeding, 129, 72-75. https://doi.org/10.1111/j.1439-0523.2009.01655.x
Michelmore, R. W., Paran, I., & Kesseli, R. V. (1991). Identification of markers linked to disease resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating
populations. Proceedings of National Academy of Science, USA, 88, 9828-9832.
Panse, V. G., & Sukhatme, P. V. (1985). Statistical methods for agricultural workers (4th ed.). ICAR, New Delhi.
Prasanth, K., Varalakshmi, B., Venugopalan, R., & Sriram, S. (2019). Screening of bitter gourd germplasm and advanced breeding lines against powdery mildew. Indian Phytopathology, 72(1), 15-22. https://doi.org/10.1007/s42360-018-0070-7
Saxena, M., Rathore, R. P. S., Gupta,R. P., Bhargav, H., & Thakur, B. (2017). Horticultural Statistics at a Glance 2017. Horticulture Statistics Division, Department of Agriculture, Cooperation & Farmers Welfare, Ministry of Agriculture & Farmers Welfare, Government of India.
Saxena, S., Singh, A., Archak, S., Behera, T. K., John J. K., Meshram, S. U., & Gaikwad, A. B. (2015). Development of novel simple sequence repeat markers in bitter gourd (Momordica charantia L.) through enriched genomic libraries and their utilization in analysis of genetic diversity and cross-species transferability. Applied Biochemistry and Biotechnology, 175, 93-118. https://doi.org/10.1007/s12010-014-1249-8
Shukla, A., Singh, V. K., Bharadwaj, D. R., Kumar, R., Rai, A. K., Mugasimangalam, R., Parameswaran, S., Singh, M., & Naik, P. S. (2015). De novo assembly of bitter gourd transcriptomes: Gene expression and sequence variations in gynoecious and monoecious lines. Plos One, 10, e0128331. https://doi.org/10.1371/journal.pone.0128331
Tan, S. P., Kha, T. C., Parks, S. E., & Roach, P. D. (2016). Bitter melon (Momordica charantia L.) bioactive composition and health benefits: A review. Food Reviews International, 32, 181–202. https://doi.org/10.1080/87559129.2015.1057843
Tetteh, A. Y., Wehner, T. C., & Davis, A. R. (2013). Inheritance of resistance to the new race of powdery mildew in watermelon. Crop Science, 53, 880-887. https://doi.org/10.2135/cropsci2012.07.0453
Vanderplank, J. E. (1963). Plant diseases: Epidemics and control (p. 349). NY: Academic Press.
Wang, Z., & Xiang, C. (2013). Genetic mapping of QTLs for horticulture traits in an F2-3 population of bitter gourd (Momordica charantia L.). Euphytica, 193, 235-250. doi:10.1007/s10681-013-0932-0
Yang, B., Li, X., Zhang, C., Yan, S., Wei, W., Wang, X., Deng, X., Qian, H., Lin, H., & Huang, W. (2015). Design, synthesis and biological evaluation of novel peptide MC2 analogues from Momordica charantia as potential anti- diabetic agents. Organic and Biomolecular Chemistry, 13, 4551-4561. doi:10.1039/C5OB00333D
Downloads
Published
Issue
Section
License
Copyright (c) 2024 K Prasanth , D C Lakshmana Reddy, S Sriram , R Venugopalan , M Pitchaimuthu , K Hima Bindu, B Varalakshmi (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
