Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification

  • K V Ravishankar ICAR-Indian Institute of Horticultural Research
  • R Vasudeva ICAR-Indian Institute of Horticultural Research
  • B Hemanth ICAR-Indian Institute of Horticultural Research
  • P Nischita ICAR-Indian Institute of Horticultural Research
  • B R Sthapit UNEP-GEF, Bioversity International, Nepal
  • V A Parthasarathy UNEP-GEF, ICAR-Indian Institute of Horticultural Research
  • V R Rao Bioversity International, Rome
Keywords: Cross-species amplification, Garcinia indica, Microsatellite markers, Next-generation sequencing (NGS)

Abstract

Garcinia indica popularly known as ‘Kokum’ or Murugalu”, is a medium-sized evergreen tree found in the western-ghats of India. This tree species is highly exploited to produce anti-obesity drugs and culinary purposes. Its population is threatened by overexploitation and loss of habitat. The development of microsatellite markers would help in understanding the genetic structure and further to develop appropriate conservation strategies. In this study, using next generation sequencing platform Illumina Hiseq 2000, we have sequenced the partial genome of G. indica and identified 3725 microsatellites. Forty-eight microsatellite markers were analyzed using 30 accessions. Polymorphism information content (PIC) values ranged from 0.718 to 0.968 with a mean value of 0.922. Allele per locus ranged from 3 to 33 per locus. The probability of identity values ranged from 0.00329 to 0.30489. Cross-species amplification SSR primers in the related species showed a moderate transferability from 12.5 % (for G. morella) to 18.7%(for G. gummigutta)

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Published
2021-06-30
How to Cite
Ravishankar, K. V., Vasudeva, R., Hemanth, B., Nischita, P., Sthapit, B. R., Parthasarathy, V. A., & Rao, V. R. (2021). Isolation and characterization of microsatellite markers from Garcinia indica and cross species amplification. Journal of Horticultural Sciences, 16(1), 125-129. Retrieved from https://jhs.iihr.res.in/index.php/jhs/article/view/1128
Section
Short Communications