Carotenoid Content in Cherry Tomatoes Correlated to the Color Space Values L*, a*, b*: A Non-destructive Method of Estimation

Shilpa  Pandurangaiah; Sadashiva A T; Shivashankar K S; SudhakarRao  D V; Ravishankar K V

doi:10.24154/jhs.v15i1.779

Authors

Shilpa Pandurangaiah Author
Sadashiva A T Author
Shivashankar K S Author
SudhakarRao D V Author
Ravishankar K V Author

DOI:

https://doi.org/10.24154/jhs.v15i1.779

Keywords:

β carotene, Carotenoid, Lycopene, Tomato

Abstract

Cherry tomatoes are rich sources of carotenoids. The carotenoids are known to be precursors of vitamin A and also act as an antioxidant. It is important to visually judge the tomato surface color for higher β carotene content since this is the major provitamin AA carotenoid. Estimation of carotenoids by HPLC (High Performance Liquid Chromatography) and spectrophotometric methods in tomatoes are very expensive and time consuming. Therefore, colorimeters can be used to describe the color and determine the carotenoid content in a relatively easy and inexpensive manner. The objective of this study was to determine, if the carotenoid content within cherry tomatoes measured by conventional method could correlate with colorimetric CIE (Commission International del’Eclairage) L*, a*, b* color space values. Strong correlations were found between color surface value a* and total carotenoids (0.82) and lycopene content (0.87). We also observed positive correlation for the b* color value with β carotene (0.86). The L* value was negatively correlated (-0.78) with an increase in carotenoids. These close associations between color space values L*, a*, b* and carotenoids will help the breeders to quickly screen large germplasm/ breeding lines in their breeding program for improvement in carotenoid content through this time saving, inexpensive and nondestructive method at fully ripe stage.

References

Radzevicius A.,Viskelis P., Viskelis J ., Karkleliene, R.,Juskeviciene, D. 2014 Tomato fruit color changes during ripening on vine. International Journal of Biological, Food, Veterinary and Agricultural Engineering, Vol : 8 (2).

Radzevicius A., Viskelis P., Bobinas C. Quality and physiological parameters of tomato (Lycopersicon esculentum Mill.) fruits of Lithuanian selection, 2008, Biologija. 54 (2):108–111.

Giuliano G., Bartley G.E., Scolnik P.A. 1993 Regulation of carotenoid biosynthesis during tomato development. The Plant Cell, 5: 379-387.

Horton B.D. and Stark F.C. 1969 Developmental rates and biosynthesis of carotenoids in tomatoes (Lycopersicone sculentum Mill.) as influenced by two solar radiation levels. Maryland Agricultural Experimental Station Bulletin. P(19).

CIE L*a*b* colour scale 1996. Hunter Lab Applications Note, 8(7): 1-4.

Itle R.A., and Kabelka E.A. 2009. Correlation Between L*a*b* Color Space Values and Carotenoid content in pumpkins and squash (Cucurbita spp.), Hort Science, 44 (3): 633–637.

Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic bio membranes. Method Enzymol, 148: 350–382.

Lopez Camelo A.F. Gómez P.A. 2004. Comparison of color indexes for tomato ripening. Horticultura Brasileira, 22(3): 534-537.

Pathare P.B., Opara U.L. and Al-Said F.AJ. 2004. Measurement and Analysis in Fresh and Processed Foods : A Review. Food Bioprocess Technol, 6: 36-60.

Serino S., Gomez L., Costagliola G., Gautier H. 2009. HPLC assay of tomato carotenoids : validation of a rapid micro extraction technique. J Agric Food Chem, 57: 8753–8760.

Thai C.N., Shewfelt R.L., Garner J.C. 1990. Tomato color changes under constant and variable storage temperatures : empirical models, Transactions of the ASAE, 33(2): 607-614.