Impact of carbohydrate metabolism pathways on bearing habit of mango (Mangifera indica L.) genotypes
Keywords:Carbohydrate metabolism, Irregular bearing, Mango, Molecular markers
Heterozygosity is the major constraint in perennial fruit crop like mango for regular bearing breeding. Majority of the popular mango varieties have irregular bearing habit. Many external and internal factors affect the bearing habit of perennial fruit crops. Among internal factors, the level of carbohydrate reserves and phytohormones plays a major role on bearing habit of fruit crops like apple, citrus, mango, litchi etc., Therefore, present research work aimed to study the carbohydrate metabolism pathways in regular and irregular mango genotypes of varying origin. A total of 30 primers were designed using in silico mining of four key genes coding for citrate synthase, alcohol dehydrogenase, sucrose phosphate synthase and trehalose phosphate synthase. These genes play important role in sugar and starch metabolism in mango. Of these specific primers, 14 showed polymorphism among the genotypes studied. Gene diversity (GD), average number of alleles per locus (An), polymorphism information content (PIC) and major allele frequency (Maf) observed were 0.45, 2.14, 0.35, 0.59, respectively. Simple sequence repeats markers grouped 63.15% studied mango genotypes of regular bearers together. Further, these markers could be utilized in a greater number of genotypes for regularity.
Bajpai, Y., Trivedi, M., Muthukumar, M. and Bajpai, A. 2021. Novel insights into biochemical and hormonal factors regulating floral transition in mango (Mangifera indica L.). Ind. J. Biotech., 20(1): 54-64.
Benny, J., Giovino, A., Marra, F.P., Balan, B., Martinelli, F., Caruso, T. and Marchese, A. 2022. Transcriptomic analysis of the Pistacia vera (L.) fruits enable the identification of genes and hormone-related gene linked to inflores cence bud abscission. Genes., 13(1): 60. DOI: https://doi.org/10.3390/genes13010060
Brownleader, M.D., Harborne, J.B.and Dey, P.M. 1997. Carbohydrate Metabolism: Primary metabolism of monosaccharides. Plant Biochem.,111. DOI: https://doi.org/10.1016/B978-012214674-9/50004-7
Coleman, H.D., Beamish, L., Reid, A., Park, J.Y. and Mansfield, S.D. 2010. Altered sucrose metabolism impacts plant biomass production and flower development. Transgenic res., 19(2): 269-283. https://doi.org/10.1007/s11248-009-9309-5 DOI: https://doi.org/10.1007/s11248-009-9309-5
Doyle, J.J., and Doyle, J.L. 1987. A rapid total DNA preparation procedure from fresh plant tissue. Focus.,12:13-15.
Eldik, G.V., Ruiter, R.K., Herpen, M. van, Schrauwen, J.A.M. and Wullems, G.J. 1998. An alcohol dehydrogenase-like gene is specifically expressed in potato pistils. J. Expert. Bot..,49(325): 1453–1454. DOI: https://doi.org/10.1093/jxb/49.325.1453
FAOSTAT 2019. Food and agriculture data. Retrieved from Food and Agriculture Organisation of the United Nations. http://www.fao.org/faostat/en/data.
Fischer, G., Almanza-Merchán, P.J. and Ramírez, F. 2012. Source-sink relationships in fruit species: A review. Rev. Colombiana de Ciencias Hortícolas., 6(2): 238-253. DOI: https://doi.org/10.17584/rcch.2012v6i2.1980
Han, D., Wang, Y., Zhang, Z., Pu, Q., Ding, H., Han, J., Fan, T., Bai, X. and Yang, G. 2017. Isolation and functional analysis of MxCS3: a gene encoding a citrate synthase in Malus xiaojinensis, with functions in tolerance to iron stress and abnormal flower in transgenic Arabidopsis thaliana. Pl. Growth Reg., 82(3): 479-489. DOI: https://doi.org/10.1007/s10725-017-0274-3
Kumar, M., Ponnuswami, V., Nagarajan, P. and Jeyakumar, P. 2013. Molecular characterization of ten mango cultivars using simple sequences repeat (SSR) markers. Af. J. Biotech., 12: 6568-6573. DOI: https://doi.org/10.5897/AJB2013.12797
Liu, K. and Spencer, V.M. 2005. Power marker: an integrated analysis environment for genetic marker analysis. Bioinformatics., 21: 2128-2129. DOI: https://doi.org/10.1093/bioinformatics/bti282
NHB 2020-21. Indian Horticulture Database. Gurugram, India. http://www.nhb.com
Sharma, N., Singh, A.K., Singh, S.K., Mahato, A.K., Srivastav, M. and Singh, N.K. 2020. Comparative RNA sequencing-based transcriptome profiling of regular bearing and alternate bearing mango (Mangifera indica L.) varieties reveals novel insights into the regulatory mechanisms underlying alternate bearing. Biotech. Lett., 42: 1035-1050. DOI: https://doi.org/10.1007/s10529-020-02863-8
Sharma, N., Singh,S.K., Mahato,A.K., Ravishankar, H., Dubey, A.K. and Singh,N.K.2019. Physiological and molecular basis of alternate bearing in perennial fruit crops. Sci. Horti., 243:214-225. DOI: https://doi.org/10.1016/j.scienta.2018.08.021
Spandana, B., Reddy, V. P., Prasanna, G. J., Anuradha, G. 2012. Development and characterization of microsatellite markers (SSR) in sesamum. (Sesamum indicum L.) species. Appl. Bioche. Biotech., 168: 1594-1607. DOI: https://doi.org/10.1007/s12010-012-9881-7
Wahl, V., Ponnu, J., Schlereth, A., Arrivault, S., Langenecker, T., Franke, A. and Schmid, M. 2013. Regulation of flowering by trehalose-6- phosphate signaling in Arabidopsis thaliana. Science., 339(6120): 704-707. DOI: https://doi.org/10.1126/science.1230406
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Copyright (c) 2023 Hatkari Vittal , Nimisha Sharma , Mukesh Shivran, Neha Sharma , Anil Kumar Dubey Dubey , Sanjay Kumar Singh , Radha Mohan Sharma , Bikram Pratap Singh, Haritha Bollinedi, Mahesh Chand Meena , Rakesh Pandey, Sridhar Gutam
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