Production of Doubled Haploids in Onion: A Review
DOI:
https://doi.org/10.24154/jhs.v9i2.178Keywords:
Onion, Allium cepa, Gynogenesis, Haploid, Chromosome DoublingAbstract
Onion suffers from high inbreeding depression and, as a result, inbreds that are developed lack genotypic and phenotypic uniformity. Gynogenesis has emerged as a potential strategy to address this drawback. Efforts have been made since the 1980s for identifying highly-responsive genotypes and for overall improvement of the protocol for bettering gynogenic frequency in onion. Besides improving media composition, identification of responsive explants and increasing the chromosome efficiency has remained a major area of focus over the years. This article purports to review progress made thus far in the induction of gynogenic haploids in onion, and challenges/opportunities associated with it.
Downloads
References
Alan, A.R., Brants, A., Cobb, E.D., Goldschmied, P.A., Mutschler, M.A. and Earle, E.D. 2004. Fecund gynogenic lines from onion (Allium cepa L.) breeding materials. Pl. Sci.,167:1055-66 DOI: https://doi.org/10.1016/j.plantsci.2004.06.007
Alan, A.R., Lim, W., Mutschler, M.A. and Earle, E.D. 2007. Complementary strategies for ploidy manipulations in gynogenic onion (Allium cepa L.). Pl. Sci., 173: 25-31 DOI: https://doi.org/10.1016/j.plantsci.2007.03.010
Anonymous. 2007. NRCOG News, Vol 11, No. 2 Blakeslee, A.F., Belling, J., Farnham, M.E. and Bergner, A.D. 1922. A haploid mutant in the Jimson weed, Datura stramonium. Sci., 55:646-647 DOI: https://doi.org/10.1126/science.55.1433.646
Bohancec, B. 2009. Doubled Haploids via Gynogenesis. In: Advances in Haploid Production in Higher Plants. Touraev, A., Forster, B.P. and Jain, S.M. (eds.). Springer-Science and Business Media B.V., The Netherlands, pp 35-46 DOI: https://doi.org/10.1007/978-1-4020-8854-4_2
Bohanec, B. 2002. Doubled haploid onions. p145-148. In: Allium crop science: Recent advances. Rabinowich, H. and Currah, L. (eds.), CABI Publishing House, Wallingford, UK DOI: https://doi.org/10.1079/9780851995106.0145
Bohanec, B. and Jakse, M. 1997.Characteristics of onion haploid induction procedure. In: Procs. 1 st Congr. Genet. Soc. Slovenia, Ljubljana, Slovenia, pp. 47-49
Bohanec, B. and Jakse, M. 1999. Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Pl. Cell Rep., 18:737-742 DOI: https://doi.org/10.1007/s002990050652
Bohanec, B., Jakse, M. and Havey, M.J. 2003. Genetic analyses of gynogenetic haploid production in onion. J. Amer. Soc. Hortl. Sci., 128:571-74 DOI: https://doi.org/10.21273/JASHS.128.4.0571
Bohanec, B., Jakse, M. and Javornik, B. 2001. Present status of haploid induction in onion: analysis of procedure and its limitations. In: Procs. 2nd Int’l. Symp. Edible Alliaceae. Armstrong, J. (ed.). Acta Hort., 555:91-98 DOI: https://doi.org/10.17660/ActaHortic.2001.555.10
Bohanec, B., Jakse, M., Ihan, A. and Javornik, B. 1995. Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Pl. Sci., 104:215-224 DOI: https://doi.org/10.1016/0168-9452(94)04030-K
Campion, B. and Alloni, C. 1990. Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Pl. Cell Tiss. Org. Cult., 20:1-6 DOI: https://doi.org/10.1007/BF00034750
Campion, B., Azzimonti, M.T., Vicini, E., Schiayi, M. and Falavigna, A. 1992. Advances in haploid induction in onion (Allium cepa L.) through in vitro gynogenesis. Pl. Sci., 86:97-104 DOI: https://doi.org/10.1016/0168-9452(92)90183-M
Campion, B., Perri, E., Azzimonti, M.T., Vicini, E. and Schiavi, M. 1995. Spontaneous and induced chromosome doubling in gynogenic lines of onion (Allium cepa L.). Pl. Breed., 114:243-46 DOI: https://doi.org/10.1111/j.1439-0523.1995.tb00802.x
Chen, J.F., Cui, L., Malik, A.A. and Mbira, K.G. 2010. In vitro haploid and dihaploid production via unfertilized ovule culture. Pl.t Cell Tiss. Org. Cult., 68:51-59 DOI: https://doi.org/10.1007/s11240-010-9874-6
Cohat, J. 1994. Obtention chez l’echalote (Allium cepa L. var. aggregatum) de plantes haploides gynogenetiques par culture in vitro de boutons floraux. Agronomie,14:229-304 DOI: https://doi.org/10.1051/agro:19940503
Dunstan, D.I. and Short, K.C. 1977. Improved growth of tissue cultures of onion (Allium cepa). Physiol. Plant., 41:70-72 DOI: https://doi.org/10.1111/j.1399-3054.1977.tb01525.x
Ebrahimi, R. and Zamani, Z. 2009. Effect of polyamines on in vitro gynogenesis of onion (Allium cepa L.). Am- Eurasian J. Sustain. Agri., 3:71-74
Forster, B.P., Heberle-Bors, E., Kasha, K.J. and Touraev, A. 2007. The resurgence of haploids in higher plants. Trends Pl. Sci., 12:368-375 DOI: https://doi.org/10.1016/j.tplants.2007.06.007
Gamborg, O.C., Miller, R.A. and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exptl. Cell Res., 50:151-58 DOI: https://doi.org/10.1016/0014-4827(68)90403-5
Geoffriau, E., Kahane, R. and Rancillac, M. 1997. Variation of gynogenesis ability in onion (Allium cepa L.). Euphytica, 94:37-44 DOI: https://doi.org/10.1023/A:1002949606450
Grzebelus, E. and Adamus, A. 2004. Effect of anti-mitotic agents on development and genome doubling of gynogenic onion (Allium cepa L.) embryos. Pl. Sci.,167:569-574 DOI: https://doi.org/10.1016/j.plantsci.2004.05.001
Jakse, M., Bohanec, B. and Ihan, A. 1996. Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants. Pl. Cell Rep., 15:934-938 DOI: https://doi.org/10.1007/BF00231591
Jakse, M., Bohanec, B. and Havey, M.J. 2003. Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos. Pl. Cell Rep., 21:905-910 DOI: https://doi.org/10.1007/s00299-003-0595-8
Jakse, M., Hirschegger, P., Bohanec, B. and Havey, M.J. 2010. Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. J. Amer. Soc. Hortl. Sci., 135:67-73 DOI: https://doi.org/10.21273/JASHS.135.1.67
Javornik, B., Bohanec, B. and Campion, B. 1998. Studies in the induction of a second cycle gynogenesis in onion (Allium cepa L.) and genetic analysis of the plant. Pl. Breed., 117:275-78 DOI: https://doi.org/10.1111/j.1439-0523.1998.tb01939.x
Keller, E.R.J. and Korzum, L. 1996. Haploidy in onion (Allium cepa L.) and other Allium species. In: In vitro haploid production in higher plants.Vol 3. Jain, S.M., Sopory, S.K. and Veilleux, R.E. (eds.). Springer Science and Business Media, B.V. Netherlands, pp. 51-75 DOI: https://doi.org/10.1007/978-94-017-1858-5_4
Keller, J. 1990. Culture of unpollinated ovules, ovaries and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.). Euphytica, 47:241-247 DOI: https://doi.org/10.1007/BF00024247
Luthar, Z. and Bohanec, B. 1999. Induction of direct somatic organogenesis in onion (Allium cepa L.) using a two- step flower or ovary culture. Pl. Cell Rep., 18:797-802 DOI: https://doi.org/10.1007/s002990050664
Martinez, L.E., Augero, C.B., Lopez, M.E. and Galmarini, C.R. 2000. Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Pl. Sci.,156:221-226 DOI: https://doi.org/10.1016/S0168-9452(00)00263-6
Michalik, B., Adamus, A. and Nowak, E. 2000. Gynogenesis in Polish onion cultivars. J. Pl. Physiol., 156:211-216 DOI: https://doi.org/10.1016/S0176-1617(00)80308-9
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15:473-97 DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Muren, R.C. 1989. Haploid plant induction from unpollinated ovaries in onion. Hort. Sci., 24:833-34 DOI: https://doi.org/10.21273/HORTSCI.24.5.833
Murovec, J. and Bohanec, B. 2012. Haploids and Doubled Haploids in Plant Breeding. In: Plant Breeding. Abdurakhmonov, I. (ed.). InTech, Europe, p87-106 DOI: https://doi.org/10.5772/29982
Musial, K., Bohanec, B. and Przywara, L. 2001. Embryological study on gynogenesis in onion (Allium cepa L.). Sex. Pl. Repr., 13:335-41 DOI: https://doi.org/10.1007/s004970100096
Ponce, M., Martinez, L. and Galmarini, C. 2006. Influence of CCC, putrescine and gellan gum concentration on gynogenic embryo induction in Allium cepa. Biol. Plant., 50:425-428 DOI: https://doi.org/10.1007/s10535-006-0061-x
Puddephat, I.J., Robinson, H.T., Smith, B.M. and Lynn, J. 1999. Influence of stock plant pre-treatment on gynogenic embryo induction from flower buds of onion. Pl. Cell Tiss. Org. Cult., 57:145-148 DOI: https://doi.org/10.1023/A:1006312614874
Downloads
Published
Issue
Section
License
Copyright (c) 2014 A S Dhatt, Prerna Thakur (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
. 
