Low Cost Strategy for Micropropagation of Lilium Asiatic Hybrid Cv. Toscana

R Kaur; Neetu Thakur; D R Sharma

doi:10.24154/jhs.v1i1.668

Authors

R Kaur Author
Neetu Thakur Author
D R Sharma Author

DOI:

https://doi.org/10.24154/jhs.v1i1.668

Keywords:

Lilium, Micropropagation, Low-Cost Strategy.

Abstract

A low cost protocol for in vitro propagation of Lilium cv. Toscana has been developed through incorporation of cost-effective media components. MS medium supplemented with 0.75 mg^-1 BAP (6-benzylaminopurine) and 0.5 mg^-1 NAA (α-naphthalene-acetic acid) was prepared with tapioca granules, table sugar and tap water in different combinations in place of agar-agar, sucrose and distilled water, respectively. Culture medium containing all the cost effective components was found to be the best for in vitro establishment of cultures yielding 6.00 bulblets per explant and medium supplemented with tapioca granules as cost effective component was found to be the best for in vitro multiplication of bulblets giving 3.70 bulblets per in vitro formed bulblet five weeks from third subculture. Tapioca supplemented MS medium containing 1 mg^-1 NAA was significantly better than all the other modified media giving 86.62% in vitro rooting, 2.86 average root number and 4.60 cm average root length. For hardening of in vitro rooted bulblets, coco peat, peat moss and coco peat in combination with peat moss were found to be at par giving 100% survival.

References

Chandra, R., Upadhyay, M. D. and Chaudhary D. R. 1990. A simplified low cost medium for potato micropropagation. In: Proc. Second Int'l. Conf. Biotech. lARI, New Delhi.

Chu, C. C, Hill, R. D. and Brule-Babel, A. L. 1990. High frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide containing media. PI. ScL, 66:255-62.

Dabrowski, J., Dabski, M., Kozak, D., Saniewski, M., Beijersbergen, J. C. M. and Bogatko, W. 1992. Influence of some growth regulators on regenration of lily bulbs in vitro. Acta Hort., 325:537-41.

Ganapathi, T. R., Mohan, J. S. S., Suprasanna, P., Bapat, V. A. and Rao, P. S. 1995. A low cost strategy for in vitro propagation of banana. Curr. ScL, 68:646-49.

Gomez, K. A and Gomez, A. A. 1984. Statistical procedures for agricultural research, p 680. S^'Edn., John Wiley & Sons, Singapore. Jeong, J. H., Lee, J. K. and Roh, M. S. 1996. In vitro propagation of bulbscale section of several Korean native lilies. Acta Hort., 414:269-76.

Kaul, S. 1998. Studies on micropropagation and hardening of kiwifruit (Actinidia deliciosa). MSc. Thesis

submitted to Dr. Y.S. Parmar University of Horticulture and Forestry, Nauni, Solan, India.

Last, D. I. and Brettell, R.I. S. 1990. Embryo yield in wheat anther culture is influenced by the choice of sugar in the culture medium. PI. Cell Rep., 9:14-16.

Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. PI, 15:473-97.

Nene, Y. L., Sheila, V. K. and Moss, J. P. 1996. Tapioca -a potential substimte For agar in tissue culture media. Curr. Set, 70:493-94.

Novak, F. J. and Petru, E. 1981. Tissue culture propagation of Lilium hybrids. Sci. Hortic, 14:191-99.

Okuno, K., Matsumoto, K. and Hisatima, S. 1996. A trial of improvement of micropropagation of Brassica compestris cultivar Kukitachina by cost stimulations. J. Soc. High Tech. Agri., 8:253-63.

Orshinky, B. R., McGregor, L. J., Johnson, G. I. E., Huel, P. and Kartha, K. K. 1990. Improved embryoid induction and green shoot regeneration from wheat anthers cultured in medium with maltose. PI. Cell. Rep., 9:365-69.

Priyadarshi, S. and Sen, S. 1992. A revised scheme for mass propagation of Easter lily. PL Cell Tissue & Organ Culture., 30:193-97.

Rybczynski, J. J. and Gomolinska, H. 1989.6- benzy-ladenine control of the initial bulblet formation of wild Hly (Lilium martagon L.). Acta Hort., 251:183-89.

Sharma, D. R., Chauhan, P. S., Kaur, R. and Srivastava, D. K. 1992. Micropropagation of colt a semi-dwarf rootstock of cherry. Indian J. Hort., 49:209-12.

Sharma, T. R. and Singh, B. M. 1995. A simple and cost effective medium for propagation of ginger {Zingiber officinale). Indian J. Agric. Sci., 67:506-08.

Sorvari, S., Toldi, O., Ahanen, K., Viinamaki, T, Hakonen, T. and Tahvonen, R. 1997. Using polysaccharide and galactomannans as gelling agents in capsule formation of artificial seeds. J. Amer. Soc. Hort. Sci., 122:878-83.

Van-Aartrijk, J. and Blom-Barnhoorn, G. J. 1983. Adventitious bud formation from bulbscale explants of Lilium speciosum Thunb: In vitro effects of wounding, TIBA and temperature. Pflanzenphysiol, 110:353-63.