A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees

Authors

  • Prakash Patil Author
  • Neeta Vastrad Author
  • M R Dinesh Author
  • A R Bantwal Author

DOI:

https://doi.org/10.24154/jhs.v2i2.613

Keywords:

Micropropagation, Mature Explants, Carica papaya

Abstract

A revised protocol has been developed for in vitro propogation of papaya using explants from field-grown trees. Successful establishment of papaya in vitro using lateral buds could be obtained by treating the buds with Carbendazim (0.2%) and Streptomycin (0.1%) for 24h, followed by surface sterilization with mercuric chloride (0.1%) for 3 minutes and culturing on MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Established buds were proliferated on modified MS medium supplemented with BAP (0.3 mg/l) and NAA (0.1 mg/l). Modified MS medium supplemented with BAP (0.3 mg/l), NAA (0.1 mg/l) and GA3 (1 mg/l) caused extensive elongation of shoots. Elongated shootlets were rooted on half-strength MS medium supplemented with BAP (0.1mg/l), NAA (0.1 mg/l) and IBA (2 mg/l). Rooted plantlets were initially hardened on a potting mixture consisting of soilrite and later on a mixture of sand, soil and FYM (1:1:1).

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Published

31-12-2007

Issue

Section

Original Research Papers

How to Cite

Patil, P., Vastrad, N., Dinesh, M. R., & Bantwal, A. R. (2007). A Revised Protocol for in Vitro Propagation of Carica papaya Using Lateral Buds from Field-Grown Trees. Journal of Horticultural Sciences, 2(2), 99-103. https://doi.org/10.24154/jhs.v2i2.613

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