Estimation of Anti-hepatic Viral Compounds in Phyllanthus amarus in vitro Cultures
DOI:
https://doi.org/10.24154/jhs.v3i1.598Keywords:
Phyllanthus amarus, HPLC, Phyllanthin, HypophyllanthinAbstract
Phyllanthus amarus Schum. and Thonn (Euphorbiaceae) is recognized commonly as 'Bhumyamlaki' in the Indian system of medicine and has been traditionally used for treating a variety of ailments, including hepatic disorders. Anti-hepatic viral compounds such as phyllanthin and hypophyllanthin were evaluated in different types of in vitro cultures of Phyllanthus amarus by High Performance Liquid Chromatography (HPLC). Among the cultures, in vitro plantlets regenerating from the nodal segment recorded higher amounts of phyllanthin and hypophyllanthin.
References
Anupum Sharma, R., Singh, S., Sukhdev, S. Handa.,1993. Estimation of phyllanthin and hypophyllanthin by HPLC in Phyllanthus amarus. Phytochem. Anal., 4: 226-229
Berlin, J.1984. Plant cell cultures – a future source of natural products. Endeavour 8: 5-8
Dhar, K, Pal A. 1988. Pyrethrin content in unorganized and organized callus cultures of Chrysanthemum cineraraefolium Vis. Fitoterapia LXIV., 336-340
Foo, L. Y. 1995. Amariinic acid and related ellagi tannins from Phyllanthus amarus. Phytochem., 39: 217-224
Foo, L. Y., Wong, H. 1992. Phyllanthusiin D, unusual hydrolysable tannin from Phyllanthus amarus. Phytochem., 31: 711-713
Hiraoka, N., Tabata, M. 1974. Studies on relationship between productivity of tropane alkaloids and differentiations in Datura meteloides. Phytochem., 13: 1671
Kamboj, V. P. 2000. Herbal medicine. Curr. Sci., 78: 35-39
Kirakosayan. A 2003. A Comparative Study of Hypericum perforatum Plants of Hypericins and Hyperforins. J. Herbs, spices & medicinal plants., 10: 73-88
Kirakosyan, A., Vardapetyan, R. R., Charchoglyan, A. G., 2000. The content of hypericin and pseudohypericin in cell cultures of Hypericum perforatum. Russ. J. Pl. Physiol., 47: 270-273
Mahalakshmi, R and Rajamani, K. 2006. Studies on genetic diversity in Phyllanthus amarus. M.Sc (Hort) thesis submitted in Tamil Nadu Agricultural University, Coimbatore
Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.
Physiol. Plant., 15: 437-497
Nadkarni, K. M. 1976. In Indian Materia Medica. Popular Prakashan, Bombay : 947-949
Rajasubramanian, S., Paradha Saradhi, P. 1997. Rapid multiplication of Phyllanthus fraternus: a plant with antihepatis viral activity. Industrial crops and Products., 6: 35
Raphael, K. R. 2002. Antimutagenic activity of Phyllanthus amarus in vitro as well as in vivo. Terarog. Carcinog. Mutagen., 22: 285-291
Row, L. C., Srinivasulu, C., Samantaray, S., Das, P., 1966. Crstalline constituents of Euphorbiaceae.V. New lignans from Phyllanthus niruri Linn: The constitution of phyllanthin. Tetrahedron., 22: 2899-2908
Sharma Tripti, Dixit. 2006. Studies on Ti-mediated transformed cultures of Artemisia annua L. Ind. J. Pharm. Sci., 68: 448-455
Shiio, I., Ohta, S., 1973. Nicotine production by tobacco callus tissues and effect of plant growth regulators. Agr. Bio. Chem., 37: 1857-1864
Syamasunder, K. V., Singh, B., Thakur, R. S., Husain, A., Kiso, Y., Hikino, H. 1985 Antihepatotoxic principles of Phyllanthus niruri herbs. J. Ethnopharmacol., 14: 41-44
Takahashi, M. and Yamada, Y. 1973. Regulation of nicotine production by auxins in tobacco cultured cells in vitro. Agr. Bio. Chem., 37: 1755-1757
Zenk, M. H. 1977. In “Plant Tissue Culture and its Biotechnological Application”, Eds. Barz, W., Reinhard, E., Zenk, M.H., p. 27, Springer-Verlag, Berlin, Heidelberg, New York
Downloads
Published
Issue
Section
License
Copyright (c) 2008 R Chitra, E Vadivel, K Rajamani (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
How to Cite
. 
