Effect of osmotic stress on in vitro plant growth hormone production by osmotolerant bacteria isolated from chilli phyto microbiome

J Prasanth; G Selvakumar; A Vijaya Gopal; D Kalaivanan

doi:10.24154/jhs.v18i2.1922

Authors

J Prasanth Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101, Andhra Pradesh, India Author
G Selvakumar ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560089, Karnataka, India Author
A Vijaya Gopal Agricultural College, Acharya N.G. Ranga Agricultural University, Bapatla - 522 101, Andhra Pradesh, India Author
D Kalaivanan ICAR-Indian Institute of Horticultural Research, Hesaraghatta Lake Post, Bengaluru - 560089, Karnataka, India Author

DOI:

https://doi.org/10.24154/jhs.v18i2.1922

Keywords:

Chilli, cytokinin, gibberellic acid, indole acetic acid, osmotolerant bacteria, PEG 8000

Abstract

The present study was conducted to determine the effect of osmotic stress on the plant growth hormone production by six osmotolerant plant growth promoting bacterial strains. These strains originated from the phytomicrobiome of chilli cultivated in the drought prone areas of Andhra Pradesh. They possessed multiple plant growth promotion traits including the ability to produce a variety of plant growth hormones. The effect of osmotic stress on the plant growth hormone production was determined by High Performance Liquid Chromatography (HPLC) under normal and in vitro osmotic stress conditions using 25% Poly Ethylene Glycol (PEG) 8000. In general, it was observed that osmotic stress impacted the plant growth hormone production of the isolates, but nevertheless plant hormones were detected in all the bacterial strains. An exception to this was the cytokinin molecule zeatin riboside, which was produced at higher levels by five of the six bacterial isolates under osmotic stressed conditions.

References

Arkhipova, T.N., Veselov, S.Y., Melentev, A.I., Martynenko, E.V., & Kudoyarova, G.R. (2006). Comparison of effects of bacterial strains differing in their ability to synthesize cytokinins on growth and cytokinin content in wheat plants. Russian Journal of Plant Physiology, 53, 507-513.

Arun K.D., Sabarinathan, K.G., Gomathy, M., Kannan, R., & Balachandar, D. (2020). Mitigation of drought stress in rice crop with plant growth-promoting abiotic stress-tolerant rice phyllosphere bacteria. Journal of Basic Microbiology, 60(9), 768–786. https://doi.org/10.1002/jobm.202000011

Ashry, N.M., Alaidaroos, B.A., Mohamed, S.A., Badr, O.A.M., El-Saadony, M.T., & Esmael, A. (2022). Utilization of drought-tolerant bacterial strains isolated from harsh soils as a plant growth-promoting rhizobacteria (PGPR). Saudi Journal of Biological Sciences, 29(3), 1760– 1769. https://doi.org/10.1016/j.sjbs.2021.10.054

Audipudi, A.V. Tulasi Bai, Sanneboyina N., Naga Raju K., Lakshmi L.B., Niloufer S. & Reddy, M.S. (2021). Impact of Pseudomonas plecoglossicida AVP1, plant growth promoting rhizobacteria on growth and physiological attributes in chilli. International Journal of Modern Agriculture, 10(3), 247-257.

Bhatt, R.M., Selvakumar, G., Upreti, K.K., & Boregowda, P.C. (2015). Effect of biopriming with Enterobacter strains on seed germination and seedling growth of tomato (Solanum lycopersicum L.) under osmotic stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 85, 63-69.

Bouremani, N., Silini, A., Bouket, A.C., Luptakova, L., Alenezi, F.N., Baranov, O., & Belbahri, L. (2022). Plant growth-promoting rhizobacteria (PGPR): A rampart against the adverse effects of drought stress. Water, 15(3), 418. https://doi.org/10.3390/w15030418

Chen, W., Gai, Y., Liu, S., Wang, R., & Jiang, X. (2010). Quantitative analysis of cytokinins in plants by high performance liquid chromatography: electron spray ionization ion trap mass spectrometry. Journal of Integrative Plant Biology, 52(10), 925–932. https://doi.org/10.1111/j.1744-7909.2010.00989.x

Cohen, A. C., Travaglia, C. N., Bottini, R., & Piccoli, P. N. (2009). Participation of abscisic acid and gibberellins produced by endophytic Azospirillum in the alleviation of drought effects in maize. Botany, 87(5), 455-462.

Creus, C. M., Sueldo, R. J., & Barassi, C. A. (2004). Water relations and yield in Azospirillum- inoculated wheat exposed to drought in the field. Canadian Journal of Botany, 82(2), 273-281.

Danish, S., Zafar-ul-Hye, M., Hussain, M., Shaaban, M., Nunez-Delgado, A., Hussain, S., & Qayyum, M. F. (2019). Rhizobacteria with ACC-deaminase activity improve nutrient uptake, chlorophyll contents and early seedling growth of wheat under PEG-induced osmotic stress. International Journal of Agriculture and Biology, 21(6), 1212-1220.

Di, Y.N., Kui, L., Singh, P., Liu, L.F., Xie, L.Y., He, L.L., & Li, F.S. (2023). Identification and characterization of Bacillus subtilis B9: A diazotrophic plant growth-promoting endophytic bacterium isolated from sugarcane root. Journal of Plant Growth Regulation, 42(3), 1720-1737.

Etminani, F., & Harighi, B. (2018). Isolation and identification of endophytic bacteria with plant growth promoting activity and biocontrol potential from wild pistachio trees. The Plant Pathology Journal, 34(3), 208–217.

Ghosh, D., Gupta, A., & Mohapatra, S. (2019). A comparative analysis of exopolysaccharide and phytohormone secretions by four drought- tolerant rhizobacterial strains and their impact on osmotic-stress mitigation in Arabidopsis thaliana. World Journal of Microbiology & Biotechnology, 35(6), 90. https://doi.org/10.1007/s11274-019-2659-0

Kumar, D. A., Sabarinathan, K. G., Kannan, R., Balachandar, D., & Gomathy, M. (2019). Isolation and characterization of drought tolerant bacteria from rice phyllosphere. International Journal of Current Microbiology and Applied Sciences, 8, 2655-2664.

Mantelin, S., & Touraine, B. (2004). Plant growth promoting bacteria and nitrate availability: impacts on root development and nitrate uptake. Journal of Experimental Botany, 55(394), 27-34.

Martins, A.O., Omena-Garcia, R.P., Oliveira, F.S., Silva, W.A., Hajirezaei, M.R., Vallarino, J.G., Ribeiro, D.M., Fernie, A.R., Nunes-Nesi, A. and Araújo, W.L. (2019). Differential root and shoot responses in the metabolism of tomato plants exhibiting reduced levels of gibberellin. Environmental and Experimental Botany, 157, 331-343.

Marulanda, A., Barea, J. M., & Azcon, R. (2009). Stimulation of plant growth and drought tolerance by native microorganisms (AM fungi and bacteria) from dry environments: mechanisms related to bacterial effectiveness. Journal of Plant Growth Regulation, 28, 115-124.

Masood, S., Zhao, X. Q., & Shen, R. F. (2020). Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization. Scientia Horticulturae, 272, 109581.

Pattnaik, S., Dash, D., Mohapatra, S., Pattnaik, M., Marandi, A. K., Das, S., & Samantaray, D. P. (2020). Improvement of rice plant productivity by native Cr (VI) reducing and plant growth promoting soil bacteria Enterobacter cloacae. Chemosphere, 240, 124895.

Raza, F. A., & Faisal, M. (2013). Growth promotion of maize by desiccation tolerant Micrococcus luteus-cp37-chp37 isolated from Cholistan desert, Pakistan. Australian Journal of Crop Science, 7(11), 1693-1698.

Selvakumar, G., Bhatt, R. M., Upreti, K. K., Bindu, G. H., & Shweta, K. (2015). Citricoccus zhacaiensis B-4 (MTCC 12119) a novel osmotolerant plant growth promoting actinobacterium enhances onion (Allium cepa L.) seed germination under osmotic stress conditions. World Journal of Microbiology and Biotechnology, 31, 833-839.

Selvakumar, G., Bindu, G.H., Bhatt, R.M., Upreti, K.K., Paul, A.M., Asha, A., Shweta, K. and Sharma, M. (2018). Osmotolerant cytokinin producing microbes enhance tomato growth in deficit irrigation conditions. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 88, 459-465.

Shahzad, R., Waqas, M., Khan, A.L., Asaf, S., Khan, M.A., Kang, S.M., Yun, B.W. and Lee, I.J. (2016). Seed-borne endophytic Bacillus amyloliquefaciens RWL-1 produces gibberellins and regulates endogenous phytohormones of Oryza sativa. Plant Physiology and Biochemistry, 106, 236-243.

Timmusk, S., & Wagner, E. G. H. (1999). The plant-growth-promoting rhizobacterium Paenibacillus polymyxa induces changes in Arabidopsis thaliana gene expression: a possible connection between biotic and abiotic stress responses. Molecular Plant-Microbe Interactions, 12(11), 951-959.

Ullah, A., Manghwar, H., Shaban. M., Khan, A.H., Akbar, A., Ali, U. and Fahad, S. (2018). Phytohormones enhanced drought tolerance in plants: a coping strategy. Environmental Science and Pollution Research, 25, 33103-33118.

Vaten, A., Soyars, C. L., Tarr, P. T., Nimchuk, Z. L., & Bergmann, D. C. (2018). Modulation of asymmetric division diversity through cytokinin and SPEECHLESS regulatory interactions in the Arabidopsis stomatal lineage. Developmental cell, 47(1), 53-66.

Waldie, T., & Leyser, O. (2018). Cytokinin targets auxin transport to promote shoot branching. Plant Physiology, 177(2), 803-818.

Zhang, M., Yang, L., Hao, R., Bai, X., Wang, Y., & Yu, X. (2020). Drought-tolerant plant growth- promoting rhizobacteria isolated from jujube (Ziziphus jujuba) and their potential to enhance drought tolerance. Plant and Soil, 452, 423-440.