Evidence for Molecular Evolutionary Conservedness of Small Heat-Shock Protein Sequence in Solanaceaeous Crops Using in silico Methods
DOI:
https://doi.org/10.24154/jhs.v8i1.342Keywords:
Small Heat-Shock Protein (sHSP), Evolutionary, Conserved, Solanaceae, MarkersAbstract
Drought and heat contribute to much of the yield decline in agricultural lands all over the world. The basic physiological responses developed against drought and heat stress overlie each other, as; both these stresses eventually lead to dehydration of the cell and to osmotic imbalance. To cope with abiotic stresses, it is necessary to understand plant responses to stresses that disturb homeostatic equilibrium at the cellular and molecular level. Although there has been remarkable progress in this with development of microarray-based expression profiling methods (together with genomic sequence data), understanding on ways to employ these data to engineer plants with improved stresstolerance is still at a nascent stage. However, these data can be used for discovering genes, functional microsatellites and regulatory elements using in silico methods. In this context, single nucleotide repeat marker sequences have been identified which is associated with small heat-shock protein sequence (sHSP) for heat tolerance in Capsicum annuum. These sHSP sequences have some structural features in common; its characteristic is that it is homologous and highly conserved. These sequences have been analyzed for molecular evolutionary conservedness in solanaceaeous crops and have been found to have a single nucleotide repeat sequence and a highly conserved sHSP sequence.
References
Ellis, R.J. and van der Vies, S.M. 1991. Molecular chaperones. Annu. Rev. Biochem., 60:321-347
Georgopoulos, C. and Welch, W.J. 1993. Role of the major heat shock proteins as molecular chaperones. Annu. Rev. Cell Biol., 9:601–634
Jakob, U., Gaestel, M., Engel, K. and Buchner, J. 1993. Small heat shock proteins are molecular chaperones. J. Biol. Chem., 268:1517–1520
Scharf, K.D., Siddique, M., Vierling, E. 2001. The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing á- Crystallin domains (Acd proteins). Cell Stress Chaperones, 6:225–237
Parsell, D.A. and Lindquist, S. 1993. The function of heat- shock proteins in stress tolerance: degradation and reactivation of proteins. Annu. Rev. Genet., 27:437–496
Vierling, E. 1991. The roles of heat-shock proteins in plants. Annu. Rev. Pl. Physiol. Pl. Mol. Biol., 42:579–620
Waters, E.R., Lee, G.J. and Vierling, E. 1996. Evolution, structure and function of the smallh e a t - s h o c k proteins in plants. J. Exptl. Bot., 47:325–338
Welch, W.J. 1993. Heat shock proteins functioning as molecular chaperones: their roles in normal and stressed cells. Philos. Trans. R. Soc. Lond. B. Biol. Sci., 339:327–333
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Copyright (c) 2013 M K Chandra Prakash, Reena Rosy Thomas, M Krishna Reddy, Sukhada Mohandas (Author)
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