Characterization of Alternaria polianthi causing leaf blight disease intuberose and standardization of its growth parameters
Keywords:
Alternaria polianthi, characterization, media, leaf blight, tuberoseAbstract
Tuberose is an ornamental crop cultivated for its long-lasting spikes and alluring scent. The incidence of leaf blight disease caused by Alternaria polianthi is the most widespread and severe, resulting in reduced growth and significant yield losses. In the present study, a pure culture was isolated from the infected leaves of tuberose and based on typical symptoms on the foliage, microscopic observation and cultural traits of the fungus, it was identified as Alternaria polianthi, which was further confirmed by molecular characterization showing 100% similarity with Alternaria sp. infecting only tuberose host. Ten different media were tested to analyze the growth parameters, among them the mean colony diameter was found highest on corn meal agar (9 cm), potato dextrose agar (8.80 cm), potato carrot agar (8.27 cm) and other colony parameters were greatly influenced by the type of the growth media. Excellent sporulation was observed on corn meal agar, potato dextrose agar and water agar. This is the first kind of study on molecular characterization of Alternaria polianthi on tuberose in India.
Downloads
References
Ambesh, B. S., Ngomle, S., Marak, T., & Das, S. (2014). Morphological and biochemical variation of some Alternaria species infected on different floricultural plants. The Ecoscan (Special Issue), 361–368.
Anonymous. (2024–2025). Horticulture statistics at a glance 2024–25. Ministry of Agriculture and Farmers’ Welfare, Government of India.
Apet, K. T., Jagdale, J. S., Baig, M. F., Wagh, S. S., & Chavan, P. G. (2014). Effect of various culture media on cultural and morphological characteristics of Alternaria alternata. Trends in Biosciences, 7(21), 3383–3385.
Arunakumara, K. T. (2006). Studies on Alternaria solani (Ellis and Martin) Jones and Grout causing early blight of tomato (Master’s thesis). University of Agricultural Sciences, Dharwad, India.
Bharathi, T. U., Kumar, R., Nair, S. A., Umamaheswari, R., Sonavane, P., Kalaivanan, D., & Rao, V. K. (2023). Evaluation of tuberose genotype IIHR 17-23SP-08 (IC0642158) for flower yield, quality, and response to biotic stress. Journal of Horticultural Science, 18(1), 60–66. https://doi.org/10.24154/jhs.v18i1.2148
Chohan, S., Perveen, R., Abid, M., Naz, M. S., & Akram, N. (2015). Morpho-physiological studies, management, and screening of tomato germplasm against Alternaria solani, the causal agent of tomato early blight. International Journal of Agriculture and Biology, 17(1), 111–118.
Doyle, J. J. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
Ginoya, C. M., & Gohel, N. M. (2015). Cultural and morphological variability among isolates of Alternaria alternata (Fr.) Keissler, incitant of fruit rot of chilli. International Journal of Plant Protection, 8(1), 118–125. https://doi.org/10.15740/HAS/IJPP/8.1/118-125
Gupta, R. P., Pathak, V. N., & Verma, O. P. (1987). Morphological, cultural and pathogenic variation in Alternaria porri (Ellis) Cif., incitant of purple blotch of onion. Zentralblatt für Mikrobiologie, 142, 155–162.
Koley, S., & Mahapatra, S. (2015). Evaluation of culture media for growth characteristics of Alternaria solani causing early blight of tomato. Journal of Plant Pathology and Microbiology, 2(1), 1–5. https://doi.org/10.4172/2157-7471.S1-005
Krishna, V. P., Suryawanshi, A. P., Prajapati, S., & Surekha, S. (2018). Effect of different culture media on growth and sporulation of Alternaria dauci causing carrot leaf blight. Journal of Pharmacognosy and Phytochemistry, 7(6), 1789–1792.
Kumar, V., Kumar, K. P., & Kumar, K. M. (2015). Study of variability and sporulation by isolates of Alternaria solani of Lycopersicon esculentum. Asian Journal of Science and Technology, 6(2), 1264–1270.
Mariappan, V., Kochu Babu, M., & Kandasamy, T. K. (1977). A leaf spot disease of tuberose (Polianthes tuberosa L.) caused by a new species of Alternaria polyanthi. Current Science, 46, 131.
Pawar, P. B., Kakade, D. S., & Vavare, K. B. (2021). Morphological and cultural characteristics of Alternaria polianthi. The Pharma Innovation, 10(10), 1053–1056.
Pipaliya, B. H., & Jadeja, K. B. (2008). Cultural variability and mancozeb sensitivity of different isolates of Alternaria burnsii. Journal of Indian Mycology and Plant Pathology, 38(1), 121–122.
Rajderkar, N. R. (1966). Certain chemical requirements for growth and sporulation of Alternaria species. Mycopathologia et Mycologia Applicata, 30(2), 172–176. https://doi.org/10.1007/BF02130367
Schmey, T., Tominello-Ramirez, C. S., Brune, C., & Stam, R. (2024). Alternaria diseases on potato and tomato. Molecular Plant Pathology, 25(3), e13435.
Singh, P., Nema, S., & Tantwai, K. (2021). Morphological and molecular characterization of Alternaria species isolated from different plants. Journal of Experimental Agriculture International, 43(6), 61–71. https://doi.org/10.9734/JEAI/2021/v43i630703
Sonavane, P., Venkataravanappa, V., Krishna Reddy, M., & R. S. P. (2023). Occurrence of Phytophthora nicotianae causing collar and root rot disease of chrysanthemum in India. Environmental Conservation Journal, 24(1), 217–224. https://doi.org/10.36953/ecj.11922310
Su, Y. Y., Qi, Y. L., & Cai, L. (2012). Induction of sporulation in plant pathogenic fungi. Mycology, 3(3), 195–200.
Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution, 38, 3022–3027.
White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, & T. J. White (Eds.), PCR protocols: A guide to methods and applications (pp. 315–322). Academic Press.
Zhu, Z. Y., Huang, X. M., & Li, Y. H. (1985). An efficient technique for inducing profuse sporulation of Alternaria solani in pure culture. Journal of Phytopathology, 4(3), 180–184.
Downloads
Published
Data Availability Statement
None
Issue
Section
License
Copyright (c) 2026 P Sonavane, S R Poojitha, T U Bharathi, P Nandeesha, S Azeez, R Kumar, R Venugopalan, M V Dhananjaya, P Tejaswini (Author)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.



. 








