Production of Doubled Haploids in Onion: A Review
DOI:
https://doi.org/10.24154/jhs.v9i2.178Keywords:
Onion, Allium cepa, Gynogenesis, Haploid, Chromosome DoublingAbstract
Onion suffers from high inbreeding depression and, as a result, inbreds that are developed lack genotypic and phenotypic uniformity. Gynogenesis has emerged as a potential strategy to address this drawback. Efforts have been made since the 1980s for identifying highly-responsive genotypes and for overall improvement of the protocol for bettering gynogenic frequency in onion. Besides improving media composition, identification of responsive explants and increasing the chromosome efficiency has remained a major area of focus over the years. This article purports to review progress made thus far in the induction of gynogenic haploids in onion, and challenges/opportunities associated with it.
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Alan, A.R., Brants, A., Cobb, E.D., Goldschmied, P.A., Mutschler, M.A. and Earle, E.D. 2004. Fecund gynogenic lines from onion (Allium cepa L.) breeding materials. Pl. Sci.,167:1055-66
Alan, A.R., Lim, W., Mutschler, M.A. and Earle, E.D. 2007. Complementary strategies for ploidy manipulations in gynogenic onion (Allium cepa L.). Pl. Sci., 173: 25-31
Anonymous. 2007. NRCOG News, Vol 11, No. 2 Blakeslee, A.F., Belling, J., Farnham, M.E. and Bergner, A.D. 1922. A haploid mutant in the Jimson weed, Datura stramonium. Sci., 55:646-647
Bohancec, B. 2009. Doubled Haploids via Gynogenesis. In: Advances in Haploid Production in Higher Plants. Touraev, A., Forster, B.P. and Jain, S.M. (eds.). Springer-Science and Business Media B.V., The Netherlands, pp 35-46
Bohanec, B. 2002. Doubled haploid onions. p145-148. In: Allium crop science: Recent advances. Rabinowich, H. and Currah, L. (eds.), CABI Publishing House, Wallingford, UK
Bohanec, B. and Jakse, M. 1997.Characteristics of onion haploid induction procedure. In: Procs. 1 st Congr. Genet. Soc. Slovenia, Ljubljana, Slovenia, pp. 47-49
Bohanec, B. and Jakse, M. 1999. Variations in gynogenic response among long-day onion (Allium cepa L.) accessions. Pl. Cell Rep., 18:737-742
Bohanec, B., Jakse, M. and Havey, M.J. 2003. Genetic analyses of gynogenetic haploid production in onion. J. Amer. Soc. Hortl. Sci., 128:571-74
Bohanec, B., Jakse, M. and Javornik, B. 2001. Present status of haploid induction in onion: analysis of procedure and its limitations. In: Procs. 2nd Int’l. Symp. Edible Alliaceae. Armstrong, J. (ed.). Acta Hort., 555:91-98
Bohanec, B., Jakse, M., Ihan, A. and Javornik, B. 1995. Studies of gynogenesis in onion (Allium cepa L.): induction procedures and genetic analysis of regenerants. Pl. Sci., 104:215-224
Campion, B. and Alloni, C. 1990. Induction of haploid plants in onion (Allium cepa L.) by in vitro culture of unpollinated ovules. Pl. Cell Tiss. Org. Cult., 20:1-6
Campion, B., Azzimonti, M.T., Vicini, E., Schiayi, M. and Falavigna, A. 1992. Advances in haploid induction in onion (Allium cepa L.) through in vitro gynogenesis. Pl. Sci., 86:97-104
Campion, B., Perri, E., Azzimonti, M.T., Vicini, E. and Schiavi, M. 1995. Spontaneous and induced chromosome doubling in gynogenic lines of onion (Allium cepa L.). Pl. Breed., 114:243-46
Chen, J.F., Cui, L., Malik, A.A. and Mbira, K.G. 2010. In vitro haploid and dihaploid production via unfertilized ovule culture. Pl.t Cell Tiss. Org. Cult., 68:51-59
Cohat, J. 1994. Obtention chez l’echalote (Allium cepa L. var. aggregatum) de plantes haploides gynogenetiques par culture in vitro de boutons floraux. Agronomie,14:229-304
Dunstan, D.I. and Short, K.C. 1977. Improved growth of tissue cultures of onion (Allium cepa). Physiol. Plant., 41:70-72
Ebrahimi, R. and Zamani, Z. 2009. Effect of polyamines on in vitro gynogenesis of onion (Allium cepa L.). Am- Eurasian J. Sustain. Agri., 3:71-74
Forster, B.P., Heberle-Bors, E., Kasha, K.J. and Touraev, A. 2007. The resurgence of haploids in higher plants. Trends Pl. Sci., 12:368-375
Gamborg, O.C., Miller, R.A. and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exptl. Cell Res., 50:151-58
Geoffriau, E., Kahane, R. and Rancillac, M. 1997. Variation of gynogenesis ability in onion (Allium cepa L.). Euphytica, 94:37-44
Grzebelus, E. and Adamus, A. 2004. Effect of anti-mitotic agents on development and genome doubling of gynogenic onion (Allium cepa L.) embryos. Pl. Sci.,167:569-574
Jakse, M., Bohanec, B. and Ihan, A. 1996. Effect of media components on the gynogenic regeneration of onion (Allium cepa L.) cultivars and analysis of regenerants. Pl. Cell Rep., 15:934-938
Jakse, M., Bohanec, B. and Havey, M.J. 2003. Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos. Pl. Cell Rep., 21:905-910
Jakse, M., Hirschegger, P., Bohanec, B. and Havey, M.J. 2010. Evaluation of gynogenic responsiveness and pollen viability of selfed doubled haploid onion lines and chromosome doubling via somatic regeneration. J. Amer. Soc. Hortl. Sci., 135:67-73
Javornik, B., Bohanec, B. and Campion, B. 1998. Studies in the induction of a second cycle gynogenesis in onion (Allium cepa L.) and genetic analysis of the plant. Pl. Breed., 117:275-78
Keller, E.R.J. and Korzum, L. 1996. Haploidy in onion (Allium cepa L.) and other Allium species. In: In vitro haploid production in higher plants.Vol 3. Jain, S.M., Sopory, S.K. and Veilleux, R.E. (eds.). Springer Science and Business Media, B.V. Netherlands, pp. 51-75
Keller, J. 1990. Culture of unpollinated ovules, ovaries and flower buds in some species of the genus Allium and haploid induction via gynogenesis in onion (Allium cepa L.). Euphytica, 47:241-247
Luthar, Z. and Bohanec, B. 1999. Induction of direct somatic organogenesis in onion (Allium cepa L.) using a two- step flower or ovary culture. Pl. Cell Rep., 18:797-802
Martinez, L.E., Augero, C.B., Lopez, M.E. and Galmarini, C.R. 2000. Improvement of in vitro gynogenesis induction in onion (Allium cepa L.) using polyamines. Pl. Sci.,156:221-226
Michalik, B., Adamus, A. and Nowak, E. 2000. Gynogenesis in Polish onion cultivars. J. Pl. Physiol., 156:211-216
Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15:473-97
Muren, R.C. 1989. Haploid plant induction from unpollinated ovaries in onion. Hort. Sci., 24:833-34
Murovec, J. and Bohanec, B. 2012. Haploids and Doubled Haploids in Plant Breeding. In: Plant Breeding. Abdurakhmonov, I. (ed.). InTech, Europe, p87-106
Musial, K., Bohanec, B. and Przywara, L. 2001. Embryological study on gynogenesis in onion (Allium cepa L.). Sex. Pl. Repr., 13:335-41
Ponce, M., Martinez, L. and Galmarini, C. 2006. Influence of CCC, putrescine and gellan gum concentration on gynogenic embryo induction in Allium cepa. Biol. Plant., 50:425-428
Puddephat, I.J., Robinson, H.T., Smith, B.M. and Lynn, J. 1999. Influence of stock plant pre-treatment on gynogenic embryo induction from flower buds of onion. Pl. Cell Tiss. Org. Cult., 57:145-148
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