Limonene extraction from the zest of Citrus sinensis, Citrus limon, Vitis vinifera and evaluation of its antimicrobial activity

Atif Khurshid Wani; Rattandeep  Singh; Tahir ul Gani Mir; Nahid Akhtar

doi:10.24154/jhs.v16i2.840

Authors

Atif Khurshid Wani lovely professional university, Phagwara, Punjab-144401, India
Rattandeep Singh lovely professional university, Phagwara, Punjab-144401, India
Tahir ul Gani Mir lovely professional university, Phagwara, Punjab-144401, India
Nahid Akhtar lovely professional university, Phagwara, Punjab-144401, India

DOI:

https://doi.org/10.24154/jhs.v16i2.840

Keywords:

Anti-microbial citrus, hydro-distillation, limonene

Abstract

Citrus rinds contain essential oils. One of the major constituents of the essential oils in the zest of different fruits like Citrus sinensis, C. limon, and Vitis vinifera is limonene. In this research, limonene was extracted by hydro-distillation method using Clevenger set up and its antimicrobial activity against certain bacterial and fungal strains was determined by using Kirby Bauer’s disc diffusion method. The primary antimicrobial screening of limonene without dilution exhibited a zone of inhibition (mm) comparable to Ampicillin (20mg/ml) and Amphotericin B (20mg/ml). The effect of pure limonene against all strains used was high as compared to the isolated samples. The MIC values also showed an expected decrease in the zone of inhibition from 1:2 to 1:8 dilutions. Based on this study, the cost-effective isolation of limonene and other essential oils is quite possible.

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References

Akhtar, N., Choudhury, N., & Kumar, N. (2017a). Antioxidant and Antimicrobial Potentials of Artemisia Indica Collected from the Nepal Region. Journal of Pharmaceutical Sciences and Research, 9(10), 1822–1826.
Akhtar, N., Choudhury, N., & Kumar, N. (2017b). Evaluation of antioxidant and antimicrobial potentials of Eclipta prostrata collected from the Nepal region. The Pharma Innovation Journal, 6(11), 4–7.
Asbahani, A. El, Miladi, K., Badri, W., Sala, M., Addi, E. H. A., Casabianca, H., Mousadik, A. El, Hartmann, D., Jilale, A., Renaud, F. N. R., Elaissari, A., & Lyon, C. B. (2015). Essential oils : From extraction to encapsulation. International Journal of Pharmaceutics, 483(1–2), 220–243. https://doi.org/10.1016/j.ijpharm.2014.12.069
Atti-Santos, A., Rossato, M., Serafini, L., Cassel, E., & Moyna, P. (2005). Extraction of essential oils from lime (Citrus latifolia Tanaka) by hydrodistillation and supercritical carbon dioxide. Brazilian Archives of Biology and Technology - BRAZ ARCH BIOL TECHNOL, 48. https://doi.org/10.1590/S1516-89132005000100020
Bacanli, M., Başaran, A. A., & Başaran, N. (2015). The antioxidant and antigenotoxic properties of citrus phenolics limonene and naringin. Food and Chemical Toxicology. https://doi.org/10.1016/j.fct.2015.04.015
Bhattacharya, I., Chakraborty, R., & Chowdhury, R. (2014). Intensification of Freeze-Drying Rate of Bacillus subtilis MTCC 2396 Using Tungsten Halogen Radiation: Optimization of Moisture Content and α-Amylase Activity. Drying Technology, 32(7), 801–812. https://doi.org/10.1080/07373937.2013.860459
Bilal Ahmad, T., Asif, A. B., Atif Khurshid, wani, Masood, A. K., & Gulzar Ahmad, S. (2020). Preparation and Characterization of SnO2 Nanoparticles for Antibacterial Properties. Nanomaterial Chemistry and Technology, 1–5. https://doi.org/10.33805/2690-2575.109
Choudhury, N., Akhtar, N., & Kumar, N. (2017). Study on methanolic extract of Ageratum conyzoides for its ability to act as an antioxidant and to suppress the microbial growth. The Pharma Innovation Journal , 6(11), 170–173.
Davidowski, S. (n.d.). The Extraction and Quantification of Limonene from Citrus Rinds Using GC / MS. 1–4.
Erasto, P., & Viljoen, A. M. (2008). Limonene - a Review: Biosynthetic, Ecological and Pharmacological Relevance. Natural Product Communications, 3(7), 1934578X0800300728. https://doi.org/10.1177/1934578X0800300728
Espina, L., Gelaw, T. K., de Lamo-Castellví, S., Pagán, R., & García-Gonzalo, D. (2013). Mechanism of bacterial inactivation by (+)-limonene and its potential use in food preservation combined processes. PloS One, 8(2), e56769–e56769. https://doi.org/10.1371/journal.pone.0056769
Gelb, M. H., Tamanoi, F., Yokoyama, K., Ghomashchi, F., Esson, K., & Gould, M. N. (1995). The inhibition of protein prenyltransferases by oxygenated metabolites of limonene and perillyl alcohol. Cancer Letters, 91(2), 169–175. https://doi.org/10.1016/0304-3835(95)03747-k
Hamid, A., Aiyelaagbe, O., & Usman, L. (2011). Essential oils: Its medicinal and pharmacological uses. Int J Curr Res, 33.
Karr, L. L. (1989). Toxic properties of d-limonene in insects and the earthworm Eisenia fetida.
Lopez-sanchez, J. A., & Pagliaro, M. (2014). Limonene: a versatile chemical of the bioeconomy. Chemical Communications, 50(97), 15288–15296. https://doi.org/10.1039/C4CC06147K
Lucchesi, M. E., Chemat, F., & Smadja, J. (2004). Solvent-free microwave extraction of essential oil from aromatic herbs : comparison with conventional hydro-distillation. 1043, 323–327. https://doi.org/10.1016/j.chroma.2004.05.083
Mahato, N., Sharma, K., Sinha, M., & Cho, M. H. (2017). Citrus waste derived nutra-/pharmaceuticals for health benefits: Current trends and future perspectives. Journal of Functional Foods, 40. https://doi.org/10.1016/j.jff.2017.11.015
Malko, M., & Wróblewska, A. (2016). The importance of R-(+)-limonene as the raw material for organic syntheses and for organic industry. 70, 198–202.
Prates, H. T., Santos, J. P., Waquil, J. M., Fabris, J. D., Oliveira, A. B., Foster, J. E., Lagoas, S., & Horizonte, B. (1998). Insecticidal Activity of Monoterpenes Against Rhyzopertha dominica ( F .) and Tribolium castaneum ( Herbst ). 34(4), 5–8.
Ramgopal, K., Sreekanteshwara, S., A, V. B. S., Vinodh, S. M., & Sesha, N. (2016). Extraction of Essential Oil D-Limonene from Sweet Orange Peels by Simple Distillation. 9(9), 16–17. https://doi.org/10.9790/5736-0909021617
Sadgrove, N., & Jones, G. (2015). A Contemporary Introduction to Essential Oils: Chemistry, Bioactivity and Prospects for Australian Agriculture. 48–102. https://doi.org/10.3390/agriculture5010048
Shaw, P. E., Moshonas, M. G., Avenue, S., & Haven, W. (1997). Quantification of Volatile Constituents in Orange Juice Drinks and Its Use for Comparison with Pure Juices by Multivariate Analysis. 501, 497–501.
Son, M., Yang, J., Cho, S., Lee, J., & Oh, H. Bin. (2018). GC – MS Method for the Quantitative Analysis of Limonene from Genetically Engineered Saccharomyces cerevisiae. 39, 1368–1372. https://doi.org/10.1002/bkcs.11607
Wang, L., & Weller, C. (2006). Recent advances in extraction of nutraceuticals from plants. Trends in Food Science & Technology, 17, 300–312. https://doi.org/10.1016/j.tifs.2005.12.004
White, D. G., & Mcdermott, P. F. (2001). Biocides , drug resistance and microbial evolution. 313–317.
Yu, X., Lin, H., Wang, Y., Lv, W., & Feng, N. (2018). d -limonene exhibits antitumor activity by inducing autophagy and apoptosis in lung cancer. 1833–1847.