Defense-related responses in mango (Mangifera indica L.) cultivars ‘Prior’ and‘Kensington’ differing in resistance to Colletotrichum asianum
DOI:
https://doi.org/10.24154/jhs.v21i1.4709Keywords:
Anthracnose, Colletotrichum asianum, disease resistance, hydrolytic enzymes, mango, phenylpropanoid pathwayAbstract
Mango is highly susceptible to anthracnose, a major postharvest disease caused by Colletotrichum spp. This study investigated the biochemical mechanisms underlying resistance in two contrasting cultivars, ‘Prior’ (moderately resistant) and ‘Kensington’ (susceptible), following inoculation with Colletotrichum asianum. ‘Prior’ exhibited significantly smaller lesion sizes compared to ‘Kensington’, indicating reduced disease severity. Higher activities of chitinase and -1, 3 glucanase were observed in ‘Prior’, suggesting their critical role in degrading fungal cell walls and conferring resistance. In contrast, elevated polyphenol oxidase and peroxidase were observed in ‘Kensington’, potentially reflecting a weaker or delayed defense response. No significant differences were noted in phenylalanine ammonia lyase and superoxide dismutase activities between the cultivars. Two-way ANOVA revealed significant temporal effects for most enzymes, with genotype×time interactions for chitinase and -1,3-glucanase. Correlation analysis showed co-regulation of these enzymes (r=0.70) and a negative association with PPO (r = -0.85). Principal component analysis (72.90% variance) clearly separated the two cultivars and reflected distinct temporal defense patterns. These findings demonstrate genotype-specific, timedependent regulation of hydrolytic and oxidative defenses, providing a basis for breeding anthracnose-resistant mango cultivars.
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Apel, K., & Hirt, H. (2004). Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–399. https://doi.org/10.1146/annurev.arplant.55.031903.141701
Chakraborty, N., Mukherjee, K., Sarkar, A., & Acharya, K. (2019). Interaction between bean and Colletotrichum gloeosporioides: Understanding through a biochemical approach. Plants, 8(9), Article 345. https://doi.org/10.3390/plants8090345
Dickerson, D. P., Pascholati, S. F., Hagerman, A. E., Butler, L. G., & Nicholson, R. L. (1984). Phenylalanine ammonia-lyase and hydroxycinnamate: CoA ligase in maize mesocotyls inoculated with Helminthosporium maydis or Helminthosporium carbonum. Physiological Plant Pathology, 25(2), 111–123. https://doi.org/10.1016/0048-4059(84)90050-X
Du, Z., & Bramlage, W. J. (1994). Superoxide dismutase activities in senescing apple fruit (Malus domestica Borkh.). Journal of Food Science, 59(3), 581–584. https://doi.org/10.1111/j.1365-2621.1994.tb05567.x
Ge, Y., Bi, Y., & Guest, D. I. (2013). Defence responses in leaves of resistant and susceptible melon (Cucumis melo L.) cultivars infected with Colletotrichum lagenarium. Physiological and Molecular Plant Pathology, 81, 13–21. https://doi.org/10.1016/j.pmpp.2012.09.002
Jiménez-Maldonado, M. I., Islas-Osuna, M. A., León-Félix, J., Tovar-Pedraza, J. M., & Muy-Rangel, M. D. (2024). Glucanases and chitinases in Mangifera indica: Identification, classification, phylogeny, and expression analysis of defense genes against Colletotrichum spp. Molecules, 29(15), Article 3556. https://doi.org/10.3390/molecules29153556
Karunanayake, L. C., Adikaram, N., Kumarihamy, B. M., Bandara, B. R., & Abayasekara, C. (2011). Role of antifungal gallotannins, resorcinols and chitinases in the constitutive defence of immature mango (Mangifera indica L.) against Colletotrichum gloeosporioides. Journal of Phytopathology, 159(10), 657–664. https://doi.org/10.1111/j.1439-0434.2011.01818.x
Lurie, S., Fallik, E., Handros, A., & Shapira, R. (1997). The possible involvement of peroxidase in resistance to Botrytis cinerea in heat-treated tomato fruit. Physiological and Molecular Plant Pathology, 50(3), 141–149. https://doi.org/10.1006/pmpp.1996.0074
Mahatma, M. K., Bhatnagar, R., Mittal, G. K., & Mahatma, L. (2011). Phenol metabolism in downy mildew resistant and susceptible genotypes of pearl millet. Archives of Phytopathology and Plant Protection, 44(7), 623–636. https://doi.org/10.1080/03235408.2010.485535
McKinney, W. (2010). Data structures for statistical computing in Python. In S. van der Walt & J. Millman (Eds.), Proceedings of the 9th Python in Science Conference (pp. 51–56). https://doi.org/10.25080/Majora-92bf1922-00a
Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426–428. https://doi.org/10.1021/ac60147a030
Mohammadi, M., & Kazemi, H. (2002). Changes in peroxidase and polyphenol oxidase activities in susceptible and resistant wheat heads inoculated with Fusarium graminearum and induced resistance. Plant Science, 162(4), 491–498. https://doi.org/10.1016/S0168-9452(01)00538-6
Niu, J., Cao, Y., Lin, X., Leng, Q., Chen, Y., & Yin, J. (2018). Field and laboratory screening of anthurium cultivars for resistance to foliar bacterial blight and the induced activities of defence-related enzymes. Folia Horticulturae, 30(1), 129–137. https://doi.org/10.2478/fhort-2018-0013
Pan, S. Q., Ye, X. S., & Kuć, J. (1991). Association of β-1,3-glucanase activity and isoform pattern with systemic resistance to blue mould in tobacco induced by stem injection with Peronospora tabacina or leaf inoculation with tobacco mosaic virus. Physiological and Molecular Plant Pathology, 39(1), 25–39. https://doi.org/10.1016/0885-5765(91)90029-H
Pellegrini, L., Rohfritsch, O., Fritig, B., & Legrand, M. (1994). Phenylalanine ammonia-lyase in tobacco: Molecular cloning and gene expression during the hypersensitive reaction to tobacco mosaic virus and the response to a fungal elicitor. Plant Physiology, 106(3), 877–886. https://doi.org/10.1104/pp.106.3.877
Prakash, O., Misra, A. K., & Kishun, R. (1996). Some threatening diseases of mango and their management. In V. P. Agnihotri, A. K. Sarbhoy, & D. V. Singh (Eds.), Management of threatening plant diseases of national importance (pp. 179–202). Malhotra Publishing House.
Prusky, D. (1996). Pathogen quiescence in postharvest diseases. Annual Review of Phytopathology, 34(1), 413–434. https://doi.org/10.1146/annurev.phyto.34.1.413
Qin, L. P., Zhang, Y., Su, Q., Chen, Y. L., Nong, Q., Xie, L., & Huang, S. L. (2019). First report of anthracnose of Mangifera indica caused by Colletotrichum scovillei in China. Plant Disease, 103(5), 1043. https://doi.org/10.1094/PDIS-11-18-1980-PDN
Salles, I. I., Blount, J. W., Dixon, R. A., & Schubert, K. (2002). Phytoalexin induction and β-1,3-glucanase activities in Colletotrichum trifolii-infected leaves of alfalfa (Medicago sativa L.). Physiological and Molecular Plant Pathology, 61(2), 89–101. https://doi.org/10.1006/pmpp.2002.0422
Selvaraj, Y., & Kumar, R. (1995). Enzymatic regulation in ripening mango fruit. Indian Journal of Horticulture, 51(4), 316–323.
Sinniah, G., Adikaram, N., & Abayasekara, C. (2012). Differential defense responses expressed in mango (Mangifera indica L.) cultivars resistant and susceptible to Colletotrichum gloeosporioides. Indian Phytopathology, 65(4), 2–6.
Subhas, S. C. (1990). Enzymatic properties associated with resistance to rust and powdery mildew in peas. Indian Journal of Horticulture, 47, 341–345.
Torres, M. A. (2010). ROS in biotic interactions. Physiologia Plantarum, 138(4), 414–429. https://doi.org/10.1111/j.1399-3054.2009.01326.x
Tovar-Pedraza, J. M., Mora-Aguilera, J. A., Nava-Díaz, C., Lima, N. B., Michereff, S. J., Sandoval-Islas, J. S., & Leyva-Mir, S. G. (2020). Distribution and pathogenicity of Colletotrichum species associated with mango anthracnose in Mexico. Plant Disease, 104(1), 137–146. https://doi.org/10.1094/PDIS-01-19-0178-RE
Ulhoa, C. J., & Peberdy, J. F. (1992). Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme and Microbial Technology, 14(3), 236–240. https://doi.org/10.1016/0141-0229(92)90072-V
Wang, L., Wang, W. X., Zeng, L., Suo, H. C., Li, C. C., Shan, J. W., & Xiong, X. Y. (2020). Characteristics and differences of polyphenol oxidase, peroxidase activities and polyphenol content in different potato (Solanum tuberosum) tubers. Applied Ecology and Environmental Research, 18(6). https://doi.org/10.15666/aeer/1806_81718187
Yang, H., Cao, G., Jiang, S., Han, S., Yang, C., Wan, X., Zhang, F., Chen, L., Xiao, J., Zhu, P., Zhang, D., He, F., & Xing, W. (2021). Identification of the anthracnose fungus of walnut (Juglans spp.) and resistance evaluation through physiological responses of resistant vs. susceptible hosts. Plant Pathology, 70(5), 1219–1229. https://doi.org/10.1111/ppa.13354
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