Validation of molecular markers associated with genic male sterile genes inbell pepper (Capsicum annuum L.)
DOI:
https://doi.org/10.24154/jhs.v21i1.4255Keywords:
Bell pepper, capsicum, genic male sterility, molecular markers, pepperAbstract
F1 hybrids in chillies and bell peppers are valued for their uniformity, high yields and stress tolerance. Genetic male sterility, a stable monogenic recessive trait, is essential for cost-effective hybrid seed production. This study validated markers for five GMS genes—ms10, ms8, ms1, ms3, and msw. While markers for ms10, ms1, and ms8 were detected in some hybrids, they require confirmation in segregating populations. Conversely, the limited use of other genes stems from a lack of reliable sterile sources and tightly linked markers. Developing more robust markers will be critical for improving breeding efficiency and genetic diversity in pepper varieties.
Downloads
References
Aulakh, P. S., Dhaliwal, M. S., Jindal, S. K., Schafleitner, R., & Singh, K. (2016). Mapping of male sterility gene ms10 in chilli pepper (Capsicum annuum L.). Plant Breeding, 135(4), 531–535. https://doi.org/10.1111/pbr.12389
Bartoszewski, G., Waszczak, C., Gawronski, P., Stepien, I., Bolibok-Bragoszewska, H., Palloix, A., Lefebvre, V., Korzeniewska, A., & Niemirowicz-Szczytt, K. (2012). Mapping of the ms8 male sterility gene in sweet pepper (Capsicum annuum L.) on chromosome P4 using PCR-based markers useful for breeding programmes. Euphytica, 186, 453–461. https://doi.org/10.1007/s10681-012-0637-9
Cheng, Q., Li, T., Ai, Y., Lu, Q., Wang, Y., Wu, L., Liu, J., Sun, L., & Shen, H. (2019). Phenotypic, genetic, and molecular function of msc-2, a genic male sterile mutant in pepper (Capsicum annuum L.). Theoretical and Applied Genetics, 133, 843–855. https://doi.org/10.1007/s00122-019-03510-1
Dhaliwal, M. S., & Jindal, S. K. (2014). Induction and exploitation of nuclear and cytoplasmic male sterility in pepper (Capsicum spp.): A review. The Journal of Horticultural Science and Biotechnology, 89(5), 471–479. https://doi.org/10.1080/14620316.2014.11513108
Doyle, J. J., & Doyle, J. L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12(13), 39–40.
Fu, D., Xiao, M., Hayward, A., Fu, Y., Liu, G., Jiang, G., & Zhang, H. (2014). Utilization of crop heterosis: A review. Euphytica, 197, 161–173. https://doi.org/10.1007/s10681-014-1103-7
Jeong, K., Choi, D., & Lee, J. (2018). Fine mapping of the genic male-sterile ms1 gene in Capsicum annuum L. Theoretical and Applied Genetics, 131, 183–191. https://doi.org/10.1007/s00122-017-2995-0
Jindal, S. K., Dhaliwal, M. S., & Meena, O. P. (2020). Molecular advancements in male sterility systems of Capsicum: A review. Plant Breeding, 139(1), 42–64. https://doi.org/10.1111/pbr.12757
Lee, J., Yoon, J. B., Han, J. H., Lee, W. P., Do, J. W., Ryu, H., Kim, S., & Park, H. G. (2010). A codominant SCAR marker linked to the genic male sterility gene (ms1) in chili pepper (Capsicum annuum). Plant Breeding, 129(1), 35–38. https://doi.org/10.1111/j.1439-0523.2009.01643.x
Lin, S. W., Shieh, H. C., Wang, Y. W., Tan, C. W., Schafleitner, R., Yang, W. J., & Kumar, S. (2015). Restorer breeding in sweet pepper: Introgressing Rf allele from hot pepper through marker-assisted backcrossing. Scientia Horticulturae, 197, 170–175. https://doi.org/10.1016/j.scienta.2015.09.036
Meena, O. P., Dhaliwal, M. S., & Jindal, S. K. (2020). Heterosis breeding in chilli pepper by using cytoplasmic male sterile lines for high-yield production with special reference to seed and bioactive compound content under temperature stress regimes. Scientia Horticulturae, 262, Article 109036. https://doi.org/10.1016/j.scienta.2019.109036
Meng, Y., & Fan, Y. (2021). Identification of nucleus sterility candidate genes using a resequencing technique in sweet pepper sterile line AB91 [Preprint, Version 5]. Research Square. https://doi.org/10.21203/rs.2.18377/v5
Naresh, P., Lin, S. W., Lin, C. Y., Wang, Y. W., Schafleitner, R., Kilian, A., & Kumar, S. (2018). Molecular markers associated with two non-allelic genic male sterility genes in peppers (Capsicum annuum L.). Frontiers in Plant Science, 9, Article 134. https://doi.org/10.3389/fpls.2018.01343
Ortega, F. A., Barchenger, D. W., Wei, B., & Bosland, P. W. (2020). Development of a genotype-specific molecular marker associated with restoration-of-fertility (Rf) in chile pepper (Capsicum annuum). Euphytica, 216, 1–10. https://doi.org/10.1007/s10681-020-2579-y
Reddy, M. K., Srivastava, A., Lin, S. W., Kumar, R., Shieh, H. C., Ebert, A. W., Chawda, N., & Kumar, S. (2015). Exploitation of AVRDC’s chili pepper (Capsicum spp.) germplasm in India. Journal of the Taiwan Society for Horticultural Science, 61, 1–9.
Schreinemachers, P., Rao, K. P. C., Easdown, W., Hanson, P., & Kumar, S. (2017). The contribution of international vegetable breeding to private seed companies in India. Genetic Resources and Crop Evolution, 64, 1037–1049. https://doi.org/10.1007/s10722-016-0423-y
Downloads
Published
Data Availability Statement
None
Issue
Section
License
Copyright (c) 2026 V Varsha, K S Timmarao, S G Hegde, P Singathiya, P Naresh, D C Lakshmana Reddy (Author); M Sood; R Rajeshwari, R Uma Maheshwari, S Mishra (Author)
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Authors retain copyright. Articles published are made available as open access articles, distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. 
This journal permits and encourages authors to share their submitted versions (preprints), accepted versions (postprints) and/or published versions (publisher versions) freely under the CC BY-NC-SA 4.0 license while providing bibliographic details that credit, if applicable.
. 
