Influence of Growth Regulators and Explant on Plant Regeneration in Tomato

Authors

  • Atul Batra Author
  • B K Banerji Author

DOI:

https://doi.org/10.24154/jhs.v6i2.419

Keywords:

Cotyledon, Explants, Genotype, Hypocotyl, Shoot Primordia

Abstract

Influence of different growth regulators was studied on in vitro growth and regeneration of tomato (Solanum esculentum) explants derived from hypocotyls and cotyledons of aseptically grown seedlings. On the basis of regeneration frequency, number of shoot primordia and shoots produced per explants, it is concluded that the best regeneration is achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg L"1 of indole-3-acetic acid and 1.0 mg L"1 zeatin. In all the genotypes studied, a good percentage of regeneration frequency was observed in hypocotyl explants used.

References

Bhatia, P., Ashwath, N., Senaratna, T. and David, M. 2004. Tissue culture studies of tomato (Lycopersicon esculentum). Pl. Cell Tiss. Org. Cult., 78:1–21

Cairney, J., Xu, N., Mackay, J. and Pullman, J. 2000. Special symposium: In vitro plant recalcitrance transcript profilg: a tool to assess the development of conifer embryos. In Vitro Cell. Dev. Biol. Pl., 36: 152-162

Faria, R.T. and Illg, R.D. 1996. Inheritance of in vitro plant regeneration ability in the tomato. Rev. Brasil. Genética, 19:113-116

Hamza, S. and Chupeau,Y. 1993. Re-evaluation of conditions for plant regeneration and Agrobacterium-mediated

transformation from tomato (Lycopersicon esculentum Mill.). J. Exptl. Bot., 44:1837-1845

Koornneeff, M., Bade, J., Hanhart, C., Horsman, K., Schel, J., Soppe, W., Verkerk, R. and Zabel, P. 1993. Characterization and mapping of a gene controlling shoot regeneration in tomato. Pl. J., 3:131-141

McCormick, S., Niedermeyer, J., Fry, J., Branason, A., Horsch, R. and Fraley,R. 1986. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Pl. Cell Rep., 5:81-84

Meredith, C.P. 1979. Shoot development in established callus cultures of cultivated tomato (Lycopersicon esculentum Mill.). Z. Pûanzenphysiol. Bd., 95:405-411

Murashige, T. and S koog, F. 1962. A revised medium for napid growth and bioassays with tabacco cultures. Physiol. Plant., 15:473-497

Nogueira, F.T.S., Costa, M.G., Figueira, M.L., Otoni, W.C. and Finger, F.L. 2001. In vitro regeneration of Santa Clara tomato plantlets and its natural mutant Firme. Sci. Agrotec. Lavras., 25:36-71

Snedecor, G.W. and Cochran, W.G. 1967. Statistical Methods. 6 ed., Iowa Univ., Iowa, USA

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Published

31-12-2011

Issue

Section

Research Papers

How to Cite

Batra, A., & Banerji, B. K. (2011). Influence of Growth Regulators and Explant on Plant Regeneration in Tomato. Journal of Horticultural Sciences, 6(2), 130-132. https://doi.org/10.24154/jhs.v6i2.419

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