Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis

Authors

  • C Rajamanickam Author
  • K Rajmohan Author

DOI:

https://doi.org/10.24154/jhs.v7i1.385

Keywords:

Banana, Ecotypes, RAPD, Molecular Markers, D2 Analysis, Genetic Divergence

Abstract

The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2 analysis too, a similar trend was observed.

References

Balasubramanyan, S., Manivannan, M.I., Vani, V., Saraswathy, S. and Rajamanickam, C. 2009. Genetic divergence in mango. Paper presented in National Seminar on Production, postharvest technology and marketing of mango, Horticultural College and Research Institute, TNAU, Periyakulam, Tamil Nadu, India, p. 28-29

Bhat, K.V. and Jarret, R.L. 1995. Random amplified polymorphic DNA and genetic diversity in Indian Musa germplasm. Genet. Resource Crop Eval., 42:107-118

Brown, P.T.H., Lang, F.D., Kranz, E. and Lorz, H. 1993. Analysis of single protoplasts and regenerated plants by PCR and RAPD technology. Mol. Gen. Genet., 237:311-317

Howell, E.C., Newbury, H.J., Swennen, R.L., Withers, L.A. and Ford-Lloyd, B.V. 1994. The use of RAPD for identifying and classifying Musa germplasm. Genome, 37:328-332

Jaccard, P. 1908. Nouvelles rescherchers sur la distribution, Florale Bull. Soc. Vaudoise de Sciences Naturelles, 44:223-270

Kahangi, E.M., Lawton, M.A. and Kumar, C.A.C.Y. 2002. RAPD profiling of some banana varieties selected by small scale farmers in Kenya. J. Hortl. Sci. Biotech., 77:393-398

Mahalanobis, P.C. 1936. On the generalized distance in statistics. Proc. Nat’l. Acad. Sci. India 2:49-55

Panse, V.G. and Sukhatme, P.V. 1967. Statistical Methods for Agricultural Workers. 2nd Edition. Indian Council of Agricultural Research, New Delhi, 356pp.

Rao, C.R. 1952. Advanced Statistical Methods in Biometrical Research. John Wiley and Sons, New York, 390pp.

Rekha, A., Ravishankar, K.V., Anand, L. and Hiremath, S.C. 2001. Genetic and genomic diversity in banana (Musa spp. and cultivars) based on D 2 analysis and RAPD markers. INFOMUSA, 10:29-34

Rai, D. and Mishra, K.K. 2005. Studies on genetic divergene in bael (Aegle marmelos Correa.). Ind. J. Hort., 62:152-154

Shanmugavelu, K.G., Aravindakshan, K. and Sathiyamurthy, S. 1992. Banana taxonomy, breeding and production technology. Metropolitan Bank Co. Pvt. Ltd., New Delhi, 459pp.

Simi, S. 2001. Molecular characterization of banana (Musa AAB plantain subgroup) clones. M.Sc. (Hort.) thesis, Kerala Agricultural University, Thrissur, 72pp.

Simmonds, N.W. 1962. The Evolution of the Bananas. Longmans, Green and Company Limited, London, 170pp.

Singh, H.P. 2007. Research and development of banana and plantain in India. Souvenir - National Conference on Banana, 25-28 October, 2007. NRCB, Trichy, Tamil Nadu, India, pp 1-19.

Singh, R., Meena, K.K. and Singh, S.K. 2003. Genetic divergence for yield and its component traits in pomegranate (Punica granatum L.). Ind. J. Pl.Genet. Resources, 16:133-134

Soni, K.B., Manju, R.V., Priya, A.O., Shimi, S. and Suseela, K.G. Molecular characterization of banana clones using RAPD markers. 2010 Global Conference on Banana, NRCB, Trichy, Tamil Nadu, India, 10-13 December, 2010, pp.1-19

Stover, R.H. and Simmonds, N.W. 1987. Bananas. Third Edition. Longman Scientific and Technical, Harlow, Esse, England, 445pp.

Valsalakumari, P.K., Nair, P.C.S. and Prabhakaran, P.V. 1985. Genetic divergence in banana. Agril. Res. J. Kerala, 22:146-149

Walbot, V. 1988. Preparation of DNA from single rice seedlings. Rice Genet. Newslett., 5:149-151

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Published

30-06-2012

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Section

Original Research Papers

How to Cite

Rajamanickam, C., & Rajmohan, K. (2012). Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis. Journal of Horticultural Sciences, 7(1), 34-40. https://doi.org/10.24154/jhs.v7i1.385

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