Resilience of French Marigold (Tagetes patula L.) Genotypes to Cold Stress: Morphological and Biochemical Insights
DOI:
https://doi.org/10.24154/jhs.v19i2.3551Keywords:
Cold stress, flowering traits, French marigold, phenolic compounds, principal component analysisAbstract
In this investigation, the impact of cold stress on four French marigold genotypes (Hisar Beauty, Hisar Jafri, Valencia Yellow, and Orange Winner) was studied under open field and polyhouse conditions. Significant reductions in vegetative and floral traits were observed, with genotype and environment interactions significantly affecting the plant performance. Hisar Beauty exhibited the highest cold resilience, with moderate reductions in plant height (12.5%), plant spread (8.6%), and leaf area (12.2%), indicating enhanced stress tolerance. In contrast, Valencia Yellow and Orange Winner showed severe declines. Cold stress also delayed bud initiation and reduced flower yield, with Hisar Beauty maintaining superior flowering traits, including higher number of flowers (61.00), flower weight (3.89 g), and stable flowering duration across environments. Hisar Beauty retained the highest total chlorophyll content (11.17 mg/g FW), with a 15.9% reduction, compared to 32.5 and 35.9% decline in Valencia Yellow and Orange Winner, respectively. Total phenolic content was also highest in Hisar Beauty (687.13 µg/g FW), significantly exceeding that of Valencia Yellow (231.02 µg/g FW) and Orange Winner (226.10 µg/g FW). The PCA indicated that cold tolerance was linked to stable vegetative growth, higher chlorophyll retention and superior flower yield, highlighting Hisar Beauty and Hisar Jafri as promising genotypes for growing in cold-prone regions.
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Amini, S., Maali-Amiri, R., Kazemi-Shahandashti, S. S., Lopez-Gomez, M., Sadeghzadeh, B., Sobhani-Najafabadi, A., & Kariman, K. (2021). Effect of cold stress on polyamine metabolism and antioxidant responses in chickpea. *Journal of Plant Physiology, 258*, 153387. https://doi.org/10.1016/j.jplph.2021.153387
https://doi.org/10.1016/j.jplph.2021.153387
Amulya, S., Singh, S. K., Verma, M. K., Patel, V. B., Thakre, M., Kumar, C., & Shankar, K. (2024). Response of selected scion and rootstock grape (Vitis spp.) genotypes to induced drought stress. *Journal of Horticultural Sciences, 19*(1), 1-9. https://doi.org/10.24154/jhs.v19i1.2370
https://doi.org/10.24154/jhs.v19i1.2370
Anonymous. (2024). Area and production of horticulture crops for 2023-24 (First Advance Estimates). Ministry of Agriculture and Farmers Welfare, Government of India, New Delhi. https://agriwelfare.gov.in/en/AgricultureEstimates
Babaei, K., Moghaddam, M., Farhadi, N., & Pirbalouti, A. G. (2021). Morphological, physiological and phytochemical responses of Mexican marigold (Tagetes minuta L.) to drought stress. *Scientia Horticulturae, 284*, 110116. https://doi.org/10.1016/j.scienta.2021.110116
https://doi.org/10.1016/j.scienta.2021.110116
Chaeikar, S. S., Marzvan, S., Khiavi, S. J., & Rahimi, M. (2020). Changes in growth, biochemical, and chemical characteristics and alteration of the antioxidant defense system in the leaves of tea clones (Camellia sinensis L.) under drought stress. *Scientia Horticulturae, 265*, 109257. https://doi.org/10.1016/j.scienta.2020.109257
https://doi.org/10.1016/j.scienta.2020.109257
Choudhary, M., Beniwal, B. S., & Kumari, A. (2014). Characterization of marigold genotypes using morphological characters. *Research on Crops, 15*(4), 839-845. https://doi.org/10.5958/2348-7542.2014.01420.X
https://doi.org/10.5958/2348-7542.2014.01420.X
Chowdhury, M. S. H., Koike, M., Muhammed, N., Halim, M. A., Saha, N., & Kobayashi, H. (2009). Use of plants in healthcare: A traditional ethno-medicinal practice in rural areas of southeastern Bangladesh. *International Journal of Biodiversity Science & Management, 5*(1), 41-51. https://doi.org/10.1080/17451590902771342
https://doi.org/10.1080/17451590902771342
Chrysargyris, A., Tzionis, A., Xylia, P., & Tzortzakis, N. (2018). Effects of salinity on Tagetes growth, physiology, and shelf life of edible flowers stored in passive modified atmosphere packaging or treated with ethanol. *Frontiers in Plant Science, 9*, 1765. https://doi.org/10.3389/fpls.2018.01765
https://doi.org/10.3389/fpls.2018.01765
De Mendiburu, F. (2014). Agricolae: Statistical procedures for agricultural research. *PeerJ, 7*(1), 1-20.
El-Attar, A. B. (2017). Is the performance of snapdragon plants (Antirrhinum majus L.) influenced by some bio-stimulators under salinity stress. *Journal of Horticultural Science & Ornamental Plants, 9*(2), 52-64. https://doi.org/10.21608/jpp.2020.122660
https://doi.org/10.21608/jpp.2020.122660
Garcia-Caparros, P., & Lao, M. T. (2018). The effects of salt stress on ornamental plants and integrative cultivation practices. *Scientia Horticulturae, 240*, 430-439. https://doi.org/10.1016/j.scienta.2018.06.022
https://doi.org/10.1016/j.scienta.2018.06.022
Gregorio, D. C. G. H., & Ham, M. D. L. M. (2007). Marigold biotechnology: Tissue culture and genetic transformation. *Transgenic Plant Journal, 1*(1), 169-174.
Hiscox, J. D., & Israelstam, G. F. (1979). A method for the extraction of chlorophyll from leaf tissue without maceration. *Canadian Journal of Botany, 57*(12), 1332-1334. https://doi.org/10.1139/b79-163
https://doi.org/10.1139/b79-163
Jan, N., Majeed, U., Andrabi, K. I., & John, R. (2018). Cold stress modulates osmolytes and antioxidant system in Calendula officinalis. *Acta Physiologiae Plantarum, 40*, 2649. https://doi.org/10.1007/s11738-018-2649-0
https://doi.org/10.1007/s11738-018-2649-0
Kumar, H., Chugh, V., Kumar, M., Gupta, V., Prasad, S., Kumar, S., & Kumar, M. (2023). Investigating the impact of terminal heat stress on contrasting wheat cultivars: A comprehensive analysis of phenological, physiological, and biochemical traits. *Frontiers in Plant Science, 14*, 1189005. https://doi.org/10.3389/fpls.2023.1189005
https://doi.org/10.3389/fpls.2023.1189005
Liu, X., Zhou, Y., Xiao, J., & Bao, F. (2018). Effects of chilling on the structure, function and development of chloroplasts. *Frontiers in Plant Science, 9*, 1715. https://doi.org/10.3389/fpls.2018.01715
https://doi.org/10.3389/fpls.2018.01715
Mazhar, A. A., Sheheed, S. I., Abdel-Aziz, N. G., & Mahgoub, M. H. (2012). Growth, flowering and chemical constituents of Chrysanthemum indicum L. plant in response to different levels of humic acid and salinity. *Journal of Applied Sciences Research, 8*(7), 3697-3706.
Mohamed, M. H., Harris, P. J. C., Henderson, J., & Senatore, F. (2002). Effect of drought stress on the yield and composition of volatile oils of drought-tolerant and non-drought-tolerant clones of Tagetes minuta. *Planta Medica, 68*(5), 472-474. https://doi.org/10.1055/s-2002-32072
https://doi.org/10.1055/s-2002-32072
Nair, S. A., Smitha, G. R., & Kalaivanan, D. (2023). Influence of container, potting media and nutrients on production and post-production consumer acceptance of potted marigold (Tagetes patula L.). *Journal of Horticultural Sciences, 18*(1), 113-121. https://doi.org/10.24154/jhs.v18i1.2153
https://doi.org/10.24154/jhs.v18i1.2153
Oh, D. H., Hong, H., Lee, S. Y., Yun, D. J., Bohnert, H. J., & Dassanayake, M. (2014). Genome structures and transcriptomes signify niche adaptation for the multiple-ion-tolerant extremophyte Schrenkiella parvula. *Plant Physiology, 164*(4), 2123-2138. https://doi.org/10.1104/pp.113.233551
https://doi.org/10.1104/pp.113.233551
Park, H. J., Kim, W. Y., & Yun, D. J. (2016). A new insight of salt stress signalling in plant. *Molecules and Cells, 39*(6), 447-459.
https://doi.org/10.14348/molcells.2016.0083
Selmar, D., & Kleinwächter, M. (2013). Influencing the product quality by deliberately applying drought stress during the cultivation of medicinal plants. *Industrial Crops and Products, 42*, 558-566. https://doi.org/10.1016/j.indcrop.2012.06.020
https://doi.org/10.1016/j.indcrop.2012.06.020
Sharma, S., Das, A., Kumari, A., & Gupta, M. M. (2024). Technological insights into lutein isolation from marigold flower and their diverse applications: A compendious review. *Phytochemistry Reviews, 24*, 321-342. https://doi.org/10.1007/s11101-024-09959-3
https://doi.org/10.1007/s11101-024-09959-3
Singleton, V. L., & Rossi, J. A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. *American Journal of Enology and Viticulture, 16*(3), 144-158.
https://doi.org/10.5344/ajev.1965.16.3.144
Sumalatha, A., Chandana, B. R., Dedhia, L., Reddy, D. L., Arivalagan, M., Bhaskar, V. V., & Tejaswini, P. (2023). Comparative analysis of BLUP and GCA for parental selection in marigold (Tagetes erecta L.) for hybrid development. *Journal of Horticultural Sciences, 18*(2), 307-314. https://doi.org/10.24154/jhs.v18i2.2106
https://doi.org/10.24154/jhs.v18i2.2106
Wu, Q., He, T., Liu, H., Luo, X., Yin, W., Chen, E., & Li, F. (2019). Cell ultrastructure and physiological changes of potato during cold acclimation. *Canadian Journal of Plant Science, 99*(6), 873-884. https://doi.org/10.1139/cjps-2019-0092
https://doi.org/10.1139/cjps-2019-0092
Yadav, S. K. (2010). Cold stress tolerance mechanisms in plants: A review. *Agronomy for Sustainable Development, 30*(3), 515-527. https://doi.org/10.1051/agro/2009050
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Copyright (c) 2024 Amala Ediga, Kanwar Pal Singh, Sapna Panwar, Namita, Sudhir Kumar, Sanjay Kumar Singh, Ramya Sree Mude, Pavnesh Kumar Verma (Author)
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