Optimization of bacoside A3 content in hydroponically cultivated Bacopa monnieri
DOI:
https://doi.org/10.24154/jhs.v20i1.3062Keywords:
GL-L, higro-super, HPLC, Medicinal Plant, nutrient film techniqueAbstract
Bacopa monnieri L. is a popular ayurvedic plant known for its cognitive properties. The active principle in B. monnieri responsible for its brain-stimulating character is a group of saponins, specifically, bacoside A (bacoside A3, bacopaside II, and bacopasaponin C). In this study, the impact of different nutrient formulations-Hoagland & Arnon (1938) (control), GL-L, Higro-Super, and Hoagland & Arnon (1950), and different light conditions viz., white LED lights (WL) & multi-spectral LED lights (ML) on the growth and bacoside A3 content of B. monnieri in a nutrient film technique (NFT) hydroponic system were investigated. The data was analyzed using the ANOVA (p<0.05) and DMRT test. It was found that GLL+ML as a treatment showed the highest number of shoots/plant (51.2±3.6), number of leaves/plant (518±21.2), leaf area (1.28±0.04), fresh weight (28.82±5.7 g/plant), and dry weight (2.2±0.26 g/plant). Conversely, the Higro-Super+ML treatment illustrated the highest bacoside A3 content (3528 mg/kg) along with suitable growth, followed by Hoagland & Arnon (1938) +ML (3204 mg/kg). These research findings shed light on the importance of considering both light spectrum and nutrient availability in optimizing growth conditions for B. monnieri cultivation
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Aggarwal, A. & Mathur A. (2020). Nexus between light and culture media on morphogenesis in Bacopa monnieri and saponin yield thereof. Heliyon, 6(10), e05245. ISSN 2405-8440, https://doi.org/10.1016/j.heliyon.2020.e05245
Babu N., S.K. Srivastawa, M. Prusty & Sahoo, T. (2016). Medicinal and Aromatic Plant Production Technologies a step towards farmwomen prosperity, Medicinal and Aromatic Plant Production Technologies report, ICAR- Central Institute for Women in Agriculture, Krishi, India.
Bansal, M., Reddy, M. S., & Kumar, A. (2016). Seasonal variations in harvest index and bacoside A contents amongst accessions of Bacopa monnieri (L.) Wettst. collected from wild populations. Physiology And Molecular Biology Of Plants : An International Journal Of Functional Plant Biology, 22(3), 407–413. https://doi.org/10.1007/s12298-016-0366-y
David Alexandre Micael Pereira, P. (2014) Antioxidant properties, traditional uses and conservation strategies. Botanical Research and Practices, Nova Science Publisher, New york.
Długosz, M. & Markowski, M. & Pączkowski, C. (2018). Source of nitrogen as a factor limiting saponin production by hairy root and suspension cultures of Calendula officinalis L. Acta Physiologiae Plantarum. 40. https://doi.org/10.1007/s11738-018-2610-2
Giurgiu, R.M., Morar, G., Dumitraș, A., Boancă, P., Duda, B.M., & Moldovan, C. (2014). Study regarding the suitability of cultivating medicinal plants in hydroponic systems in controlled environment. Research Journal of Agricultural Science, 46, 84-92.
Gowthami, R., Sharma, N., Pandey, R. et al. 2021. Status and consolidated list of threatened medicinal plants of India. Genet Resour Crop Evol 68, 2235–2263. https://doi.org/10.1007/s10722-021-01199-0
Hogewoning S. W., Trouwborst G., Maljaars H., Poorter H., Ieperen V. M., Harbinson J. (2010). Blue light dose–responses of leaf photosynthesis, morphology, and chemical composition of Cucumis sativus grown under different combinations of red and blue light, Journal of Experimental Botany, Volume 61, Issue 11, Pages 3107–3117. https://doi.org/10.1093/jxb/erq132
Joshi, T., Gupta, A., Kumar, P., Singh, A., & Kumar, A. (2021). Bacopa monnieri (Brahmi). Naturally Occurring Chemicals Against Alzheimer's Disease , Belwa, T., Seyed M. N. Academic Press, Elsevire, Iran, Chapter 3.2.4.
Karataş, M., Aasim, M., & Dazkirli, M. (2016). Influence of light-emitting diodes and benzylaminopurin on adventitious shoot regeneration of water hyssop (Bacopa monnieri (l.) Pennell) in vitro. Archives of Biological Sciences, 68(3), 501–508.
Kaur, A. C. (2022). Nutrient management of Brahmi (Bacopa monnieri L.) cultivars in soil and comparison with hydroponically grown plants for better herbage yield. Ecology, Environment and Conservation, 28(04), 1720–1725.
Kumar, P., & Saini, S. (2020). Nutrients for Hydroponic Systems in Fruit Crops. Urban Horticulture - Necessity of the Future, IntechOpen.
Maikami, M., Kanto, U., Sonjaroon, W. & Promdang, S. (2022). Comparative effects of organic and inorganic fertilizers on growth, antioxidant activity and bacoside content in Bacopa monnieri (L.) Wettst. International Journal of Agriculture Technology, 8(4):1683-1700.
Maneeply, C., Sujipuli, K., & Kunpratum, N. (2018). Growth of Brahmi (Bacopa monnieri L.) Wettst. by NFT and DFT hydroponic systems and their accumulation of saponin bacosides. NU. International Journal of Science, 15, 114-124.
Patel, S. & Birwal, P. & Basu, S. & Pawar, D. & Datir, R. & Deshmukh, G. (2018). Brahmi (Bacopa monnieri) as functional food ingredient in food processing industry. Journal of Pharmacognosy and Phytochemistry, 7. 189-194.
Pomoni, D. I., Koukou, M. K., Vrachopoulos, M. Gr., & Vasiliadis, L. (2023). A Review of Hydroponics and Conventional Agriculture Based on Energy and Water Consumption, Environmental Impact, and Land Use. Energies, 16(4), 1690. https://doi.org/10.3390/en16041690
Ryo, M., & Keiko, O. & Kazuhiro, F. & Kenji, K. (2007). Analysis of the relationship between blue‐light photon flux density and the photosynthetic properties of spinach (Spinacia oleracea L.) leaves with regard to the acclimation of photosynthesis to growth irradiance. Soil Science & Plant Nutrition, 53. 459 - 465. https://doi.org/10.1111/j.1747-0765.2007.00150.x
Szakiel, A. & C, Pączkowski & Henry, M. (2011). Influence of Environmental Abiotic Factors on the Content of Saponin in Plants. Phytochemistry Reviews, 10. 493-502. 10.1007/s11101-010-9177-x
Verma, N., & Shukla, S. (2015). Impact of various factors responsible for fluctuation in plant secondary metabolites. Journal of Applied Research on Medicinal and Aromatic Plants, 2(4):105-113. https://doi.org/10.1016/j.jarmap.2015.09.002
Watcharatanon, K. & Ingkaninan, K. & Putalun, W. (2019). Improved triterpenoid saponin glycosides accumulation in in vitro culture of Bacopa monnieri (L.) Wettst with precursor feeding and LED light exposure. Industrial Crops and Products. 134. 303-308. https://doi.org/10.1016/j.indcrop.2019.04.011
Zhang, Y.-H., Zhong, J.-J. & Yu, J.-T. (1996). Effect of Nitrogen Source on Cell Growth and Production of Ginseng Saponin and Polysaccharide in Suspension Cultures of Panax notoginseng. Biotechnol Progress, 12: 567-571. https://doi.org/10.1016/S0032-9592(98)00030-2
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Copyright (c) 2025 Mrunal Saste, Vikas Randhe, Sunita Singh, Vishal Warke, Girish Mahajan, Manish Raut, Uday Annapure, Gayatri Karri, Debjani Dasgupta (Author)
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