In vitro multiplication of clonal apple rootstock MM111

M Lal; M Jamwal; Y Sood; P Bakshi; N Sharma; B K Sinha; R Sharma

doi:10.24154/jhs.v20i2.2902

Authors

M Lal Chandigarh University , Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author
M Jamwal skuast j , Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author
Y Sood Chandigarh University , University of Agricultural Sciences, Bangalore Author
P Bakshi Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author
N Sharma Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author
B K Sinha Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author
R Sharma Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu Author

DOI:

https://doi.org/10.24154/jhs.v20i2.2902

Keywords:

Apple, clonal rootstock, in vitro, MM111

Abstract

The present investigation was carried out in five stages with an aim to establish the rapid and most economically viable methodology to facilitate in vitro propagation of clonal rootstock MM111 of apple using shoot tip and nodal segment as explants (0.5 to 0.75 cm). For culture establishment, MS medium was used either alone or in combination with 1.0 to 3.0 mg/L BAP and 1.0 to 3.0 mg/L GA3. Rooting medium involved half-strength MS medium with 0.5 to 2.0 mg/L NAA and 0.5 to 2.0 mg/L IBA along with 0.2% activated charcoal. The highest culture establishment (61.11 and 77.66%) in minimum days (32.20 and 25.40) was recorded with MS + 1.0 mg/L BAP + 1.0 mg/L GA3 for shoot tip and nodal segment explants, respectively. Maximum number of shoots (2.43 and 5.43) and shoot length (3.21 and 3.47 cm) were recorded using MS + 1.0 mg/L BAP + 1.0 mg/L GA3 with an addition of 0.2% NAA. The media composition involving half-strength MS + 1.0 mg/L NAA produced the highest rooted cultures (66.67%) in fewest days (13.00) amongst all the media compositions. Furthermore, the maximum survival rate (76.67%) of in vitro raised plantlets was observed with soil + sand + vermiculite + FYM (1:1:1:1, v/v/v/v).

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Author Biographies

M Lal, Chandigarh University, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Chandigarh University
M Jamwal, skuast j, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Directorate of Research
Y Sood, Chandigarh University, University of Agricultural Sciences, Bangalore

University Institute of Agricultural Sciences
P Bakshi, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Division of Fruit Science
N Sharma, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Division of Fruit Science
B K Sinha, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Division of Plant Physiology
R Sharma, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu

Division of Fruit Science

References

Ahmed, S., Sharma, A., Singh, A. K., Wali, V. K., & Preeti, K. (2014). In vitro multiplication of banana (Musa sp.) cv. Grand Naine. African Journal of Biotechnology, 13(27), 2696–2703. https://doi.org/10.5897/AJB2014.13750

Anonymous. (2024). Area and production of apple. Ministry of Agriculture and Farmers Welfare, Government of India. https://pib.gov.in/PressReleasePage.aspx?PRID=2012191

Boudabous, M., Marzougui, N., & Ferchichi, A. (2010). Micropropagation of apple (Malus domestica L.) cultivar Douce de Djerba through in vitro culture of axillary buds. Acta Botanica Gallica, 157(3), 513–524. https://doi.org/10.1080/12538078.2010.10516227

Castillo, A., Cabrera, D., Rodríguez, P., Zoppolo, R., & Robinson, T. (2015). In vitro micropropagation of CG41 apple rootstock. Acta Horticulturae, 1083, 569–576. https://doi.org/10.17660/ActaHortic.2015.1083.76

Dalal, A. M., Das, B., Sharma, K. A., Mir, A. M., & Sounduri, S. A. (2006). In vitro cloning of apple (Malus domestica Borkh.) employing forced shoot tip cultures on M9 rootstock. Indian Journal of Biotechnology, 5, 543–550.

Ghanbari, A. (2014). Impacts of plant growth regulators and culture media on in vitro propagation of three apple (Malus domestica Borkh.) rootstocks. Iranian Journal of Genetics and Plant Breeding, 3(1), 11–20.

Guadie, D., Bekele, T., Disasa, T., & Feyissa, T. (2016). Micropropagation of two varieties of apple (Malus domestica Borkh.) using shoot explants. Ethiopian Journal of Science, 39(2), 76–85.

Karwa, A. (2003). In vitro propagation of Citrus reticulata Blanco (Nagpur mandarin). Indian Journal of Genetics and Plant Breeding, 63, 187–188.

Kour, K., & Singh, B. (2012). In vitro multiplication of rough lemon (Citrus jambhiri Lush.). Journal of Agriculture and Veterinary Sciences, 1, 5–9. https://doi.org/10.9790/2380-01040509

Kakimzhanova, A., Dyussembekova, D., Nurtaza, A., Yessimselitova, A., Shevtsov, A., Lutsay, V., Ramakulov, Y., & Kabieva, S. (2023). An efficient micropropagation system for the vulnerable wild apple species, Malus sieversii, and confirmation of its genetic homogeneity. Erwerbs-Obstbau, 65, 621–632. https://doi.org/10.1007/s10341-022-00720-8

Kermani, M. J., Hosseini, Z. S., & Habashi, A. A. (2009). A refined tissue culture medium for in vitro proliferation of apple rootstocks. Acta Horticulturae, 829, 313–318. https://doi.org/10.17660/ActaHortic.2009.829.48

Lal, M., Jamwal, M., Sood, Y., Bakshi, P., Sharma, N., Sharma, S., & Kumar, S. (2023). Micropropagation of fruit crops: A review. Plant Science Today, 10(1), 108–117. https://doi.org/10.14719/pst.1891

Lal, M., Jamwal, M., Bakshi, P., Jasrotia, A., Sharma, N., Sharma, M., Singh, P., Sharma, S., & Kumar, S. (2021). Influence of antioxidants on in vitro culture establishment of clonal apple rootstocks. Biological Forum – An International Journal, 13(2), 381–385.

Lal, M., Jamwal, M., Sharma, M., Bakshi, P., Jasrotia, A., Kumar, A., & Deep, J. B. (2021). Economics of in vitro grown plantlets of clonal apple MM-106 rootstock. Biological Forum – An International Journal, 13(2), 332–335.

Lizarraga, A., Fraga, M., Ascasibar, J., & Gonzalez, M. (2017). In vitro propagation and recovery of eight apple and two pear cultivars held in a germplasm bank. American Journal of Plant Sciences, 8, 2238–2254. https://doi.org/10.4236/ajps.2017.89150

Modgil, M., Gupta, R., & Thakur, M. (2010). In vitro rooting and hardening in apple rootstock EMLA111. Acta Horticulturae, 865, 339–344. https://doi.org/10.17660/ActaHortic.2010.865.47

Ma, J., Fan, J., Zhang, W., Zhou, R., Shen, Y., Peng, Q., Li, M., & Lei, C. (2024). Tissue culture response and in vitro plant regeneration of Malus ‘Baiyun’. Plants, 13, 1–11. https://doi.org/10.3390/plants13152080

Renu, S., Bhattacharya, A., & Singh, R. (2018). Optimization of micropropagation from nodal segments of apple (Malus × domestica Borkh.) cultivars Golden Delicious and Red Fuji. Current Journal of Applied Science and Technology, 31(3), 1–9. https://doi.org/10.9734/CJAST/2018/45902

Sharma, M., Modgil, M., & Sharma, D. R. (2000). Successful propagation in vitro of apple rootstock MM106 and influence of phloroglucinol. Indian Journal of Experimental Biology, 38, 1236–1240.

Sharma, T., Modgil, M., & Thakur, M. (2007). Factors affecting induction and development of in vitro rooting of apple rootstocks. Indian Journal of Experimental Biology, 45, 824–829.

Singh, A. K., & Pandey, S. N. (2004). Genotypic variation among strawberry cultivars for shoot organogenesis. Acta Horticulturae, 662, 277–280. https://doi.org/10.17660/ActaHortic.2004.662.40

Skoog, F., & Miller, C. O. (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symposium of the Society for Experimental Biology, 11, 118–130.

Shabani, Z., Moghadam, E. G., Abedi, B., & Tehranifar, A. (2015). Effect of media and plant growth regulators on micropropagation of Myrobalan 29C rootstock. Journal of Horticulture and Forestry, 7(3), 57–64. https://doi.org/10.5897/JHF2014.0379

Soni, M., Thakur, M., & Modgil, M. (2010). In vitro multiplication of Merton I 793: An apple rootstock suitable for replantation. Indian Journal of Biotechnology, 10, 362–368.